Details
| Original language | English |
|---|---|
| Pages (from-to) | 103-111 |
| Number of pages | 9 |
| Journal | CRYOBIOLOGY |
| Volume | 71 |
| Issue number | 1 |
| Early online date | 14 May 2015 |
| Publication status | Published - 1 Aug 2015 |
Abstract
Multipotent stromal cells derived from the common marmoset monkey Callithrix jacchus (cjMSCs) possess high phylogenetic similarity to humans, with a great potential for preclinical studies in the field of regenerative medicine. Safe and effective long-term storage of cells is of great significance to clinical and research applications. Encapsulation of such cell types within alginate beads that can mimic an extra-cellular matrix and provide a supportive environment for cells during cryopreservation, has several advantages over freezing of cells in suspension. In this study we have analysed the effect of dimethyl sulfoxide (Me2SO, 2.5-10%, v/v) and pre-freeze loading time of alginate encapsulated cjMSCs in Me2SO (0-45. min) on the viability and metabolic activity of the cells after freezing using a slow cooling rate (-1. °C/min). It was found that these parameters affect the stability and homogeneity of alginate beads after thawing. Moreover, the cjMSCs can be frozen in alginate beads with lower Me2SO concentration of 7.5% after 30. min of loading, while retaining high cryopreservation outcome. We demonstrated the maximum viability, membrane integrity and metabolic activity of the cells under optimized, less cytotoxic conditions. The results of this study are another step forward towards the application of cryopreservation for the long-term storage and subsequent applications of transplants in cell-based therapies.
Keywords
- Alginate bead, Common marmoset, Cryopreservation, Dimethyl sulfoxide, Encapsulation, High voltage, Ice formation, Multipotent stromal cells, Stem cells, Viability
ASJC Scopus subject areas
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In: CRYOBIOLOGY, Vol. 71, No. 1, 01.08.2015, p. 103-111.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Multipotent stromal cells derived from common marmoset Callithrix jacchus within alginate 3D environment
T2 - Effect of cryopreservation procedures
AU - Gryshkov, Oleksandr
AU - Hofmann, Nicola
AU - Lauterboeck, Lothar
AU - Pogozhykh, Denys
AU - Mueller, Thomas
AU - Glasmacher, Birgit
N1 - Funding Information: Statement of funding: This study was in part supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, EXC 62/1). Funding Information: This research was in part supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation, EXC 62/1) through a scholarship by the Cluster of Excellence REBIRTH. The authors thank Julia Struss, Debapriya Saha and Anamika Chatterjee for technical assistance and Prof. S. Schlatt for the kind donation of placental material.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Multipotent stromal cells derived from the common marmoset monkey Callithrix jacchus (cjMSCs) possess high phylogenetic similarity to humans, with a great potential for preclinical studies in the field of regenerative medicine. Safe and effective long-term storage of cells is of great significance to clinical and research applications. Encapsulation of such cell types within alginate beads that can mimic an extra-cellular matrix and provide a supportive environment for cells during cryopreservation, has several advantages over freezing of cells in suspension. In this study we have analysed the effect of dimethyl sulfoxide (Me2SO, 2.5-10%, v/v) and pre-freeze loading time of alginate encapsulated cjMSCs in Me2SO (0-45. min) on the viability and metabolic activity of the cells after freezing using a slow cooling rate (-1. °C/min). It was found that these parameters affect the stability and homogeneity of alginate beads after thawing. Moreover, the cjMSCs can be frozen in alginate beads with lower Me2SO concentration of 7.5% after 30. min of loading, while retaining high cryopreservation outcome. We demonstrated the maximum viability, membrane integrity and metabolic activity of the cells under optimized, less cytotoxic conditions. The results of this study are another step forward towards the application of cryopreservation for the long-term storage and subsequent applications of transplants in cell-based therapies.
AB - Multipotent stromal cells derived from the common marmoset monkey Callithrix jacchus (cjMSCs) possess high phylogenetic similarity to humans, with a great potential for preclinical studies in the field of regenerative medicine. Safe and effective long-term storage of cells is of great significance to clinical and research applications. Encapsulation of such cell types within alginate beads that can mimic an extra-cellular matrix and provide a supportive environment for cells during cryopreservation, has several advantages over freezing of cells in suspension. In this study we have analysed the effect of dimethyl sulfoxide (Me2SO, 2.5-10%, v/v) and pre-freeze loading time of alginate encapsulated cjMSCs in Me2SO (0-45. min) on the viability and metabolic activity of the cells after freezing using a slow cooling rate (-1. °C/min). It was found that these parameters affect the stability and homogeneity of alginate beads after thawing. Moreover, the cjMSCs can be frozen in alginate beads with lower Me2SO concentration of 7.5% after 30. min of loading, while retaining high cryopreservation outcome. We demonstrated the maximum viability, membrane integrity and metabolic activity of the cells under optimized, less cytotoxic conditions. The results of this study are another step forward towards the application of cryopreservation for the long-term storage and subsequent applications of transplants in cell-based therapies.
KW - Alginate bead
KW - Common marmoset
KW - Cryopreservation
KW - Dimethyl sulfoxide
KW - Encapsulation
KW - High voltage
KW - Ice formation
KW - Multipotent stromal cells
KW - Stem cells
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=84937733236&partnerID=8YFLogxK
U2 - 10.1016/j.cryobiol.2015.05.001
DO - 10.1016/j.cryobiol.2015.05.001
M3 - Article
C2 - 25980899
AN - SCOPUS:84937733236
VL - 71
SP - 103
EP - 111
JO - CRYOBIOLOGY
JF - CRYOBIOLOGY
SN - 0011-2240
IS - 1
ER -