Details
| Original language | English |
|---|---|
| Title of host publication | Commercial and Biomedical Applications of Ultrafast Lasers VI |
| Subtitle of host publication | 22 - 25 January 2006, San Jose, California, USA |
| Place of Publication | Bellingham |
| Publisher | SPIE |
| ISBN (print) | 0-8194-6150-4 |
| Publication status | Published - 28 Feb 2006 |
| Event | Lasers and Applications in Science and Engineering - San Jose, California, United States Duration: 21 Jan 2006 → 26 Jan 2006 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Publisher | SPIE |
| Volume | 6108 |
| ISSN (Print) | 0277-786X |
Abstract
Multiphoton microscopy is a very promising method for 3D imaging of living cells. The fluorochromes are solely excited at the laser focus by multiphoton absorption using near-infrared femtosecond laser pulses. The arising fluorescence serves for a pixel-to-pixel imaging with a resolution in the submicron range. At higher laser powers, the multiphoton absorption creates a micro plasma which induces an outwardly propagating shock wave. The rapidly expanding cavitation bubble causes disruption of the material, with hardly any interaction with the surrounding tissue as the optical breakdown proceeds faster than the thermal conduction. This combination offers the possibility of simultaneous manipulation and analysis of living cells or cell organelles. Manipulation is achieved using laser pulses with an energy of a few nanojoules while imaging is done at less than 1 n J. The obtained resolution allows the precise cutting of single cell organelles without compromising the cells' viability. Thus, the implementation is excellently suited for cell surgery. We conducted ablation of different subcellular structures, like mitochondria, at different pulse energies within living cells while studying cell viability.
Keywords
- Cell surgery, Femtosecond laser, Fluorescence microscopy, Live cell, Multiphoton
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
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Commercial and Biomedical Applications of Ultrafast Lasers VI: 22 - 25 January 2006, San Jose, California, USA. Bellingham: SPIE, 2006. (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6108).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Multiphoton microscopy for cell surgery
AU - Baumgart, J.
AU - Heisterkamp, A.
AU - Ngezahayo, A.
AU - Ertmer, W.
AU - Lubatschowski, H.
PY - 2006/2/28
Y1 - 2006/2/28
N2 - Multiphoton microscopy is a very promising method for 3D imaging of living cells. The fluorochromes are solely excited at the laser focus by multiphoton absorption using near-infrared femtosecond laser pulses. The arising fluorescence serves for a pixel-to-pixel imaging with a resolution in the submicron range. At higher laser powers, the multiphoton absorption creates a micro plasma which induces an outwardly propagating shock wave. The rapidly expanding cavitation bubble causes disruption of the material, with hardly any interaction with the surrounding tissue as the optical breakdown proceeds faster than the thermal conduction. This combination offers the possibility of simultaneous manipulation and analysis of living cells or cell organelles. Manipulation is achieved using laser pulses with an energy of a few nanojoules while imaging is done at less than 1 n J. The obtained resolution allows the precise cutting of single cell organelles without compromising the cells' viability. Thus, the implementation is excellently suited for cell surgery. We conducted ablation of different subcellular structures, like mitochondria, at different pulse energies within living cells while studying cell viability.
AB - Multiphoton microscopy is a very promising method for 3D imaging of living cells. The fluorochromes are solely excited at the laser focus by multiphoton absorption using near-infrared femtosecond laser pulses. The arising fluorescence serves for a pixel-to-pixel imaging with a resolution in the submicron range. At higher laser powers, the multiphoton absorption creates a micro plasma which induces an outwardly propagating shock wave. The rapidly expanding cavitation bubble causes disruption of the material, with hardly any interaction with the surrounding tissue as the optical breakdown proceeds faster than the thermal conduction. This combination offers the possibility of simultaneous manipulation and analysis of living cells or cell organelles. Manipulation is achieved using laser pulses with an energy of a few nanojoules while imaging is done at less than 1 n J. The obtained resolution allows the precise cutting of single cell organelles without compromising the cells' viability. Thus, the implementation is excellently suited for cell surgery. We conducted ablation of different subcellular structures, like mitochondria, at different pulse energies within living cells while studying cell viability.
KW - Cell surgery
KW - Femtosecond laser
KW - Fluorescence microscopy
KW - Live cell
KW - Multiphoton
UR - http://www.scopus.com/inward/record.url?scp=33646015060&partnerID=8YFLogxK
U2 - 10.1117/12.645699
DO - 10.1117/12.645699
M3 - Conference contribution
AN - SCOPUS:33646015060
SN - 0-8194-6150-4
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Commercial and Biomedical Applications of Ultrafast Lasers VI
PB - SPIE
CY - Bellingham
T2 - Lasers and Applications in Science and Engineering
Y2 - 21 January 2006 through 26 January 2006
ER -