Details
| Original language | English |
|---|---|
| Article number | e202200129 |
| Number of pages | 10 |
| Journal | Journal of Biophotonics |
| Volume | 15 |
| Issue number | 10 |
| Early online date | 8 Jul 2022 |
| Publication status | Published - 3 Oct 2022 |
Abstract
We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement. The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma. The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements. The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS. The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane. We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
Keywords
- multimodal setup, optical coherence tomography, Raman spectroscopy, skin cancer diagnostics, ultrasound
ASJC Scopus subject areas
- Chemistry(all)
- General Chemistry
- Materials Science(all)
- General Materials Science
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Engineering(all)
- General Engineering
- Physics and Astronomy(all)
- General Physics and Astronomy
Sustainable Development Goals
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In: Journal of Biophotonics, Vol. 15, No. 10, e202200129, 03.10.2022.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy
AU - Fedorov Kukk, Anatoly
AU - Wu, Di
AU - Gaffal, Evelyn
AU - Panzer, Rüdiger
AU - Emmert, Steffen
AU - Roth, Bernhard
N1 - Funding Information: Deutsche Forschungsgemeinschaft, Grant/Award Numbers: RO 3471/18‐1, EM 63/13‐1; German Research Foundation (DFG) under Germany's Excellence Strategy within the Cluster of Excellence PhoenixD, Grant/Award Number: EXC 2122, Project ID 390833453 Funding information Funding Information: The authors acknowledge financial support from the German Research Foundation DFG (German Research Foundation, Project ID RO 3471/18‐1 and EM 63/13‐1). Also, financial support from the German Research Foundation (DFG) under Germany's Excellence Strategy within the Cluster of Excellence PhoenixD (EXC 2122, Project ID 390833453) is acknowledged.
PY - 2022/10/3
Y1 - 2022/10/3
N2 - We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement. The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma. The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements. The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS. The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane. We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
AB - We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement. The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma. The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements. The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS. The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane. We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
KW - multimodal setup
KW - optical coherence tomography
KW - Raman spectroscopy
KW - skin cancer diagnostics
KW - ultrasound
UR - http://www.scopus.com/inward/record.url?scp=85135020920&partnerID=8YFLogxK
U2 - 10.1002/jbio.202200129
DO - 10.1002/jbio.202200129
M3 - Article
C2 - 35802400
AN - SCOPUS:85135020920
VL - 15
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 10
M1 - e202200129
ER -