Details
Original language | English |
---|---|
Pages (from-to) | 1109-1121 |
Number of pages | 13 |
Journal | ACS NANO |
Volume | 5 |
Issue number | 2 |
Publication status | Published - 10 Jan 2011 |
Externally published | Yes |
Abstract
Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic-fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).
Keywords
- cell sorting, magnetic clusters, magnetic nanoparticles, magnetic-fluorescent nanostructures, quantumdots, tumor labeling
ASJC Scopus subject areas
- Materials Science(all)
- General Materials Science
- Engineering(all)
- General Engineering
- Physics and Astronomy(all)
- General Physics and Astronomy
Sustainable Development Goals
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In: ACS NANO, Vol. 5, No. 2, 10.01.2011, p. 1109-1121.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Multifunctional Nanobeads Based on Quantum Dots and Magnetic Nanoparticles: Synthesis and Cancer Cell Targeting and Sorting
T2 - Synthesis and cancer cell targeting and sorting
AU - Di Corato, Riccardo
AU - Bigall, Nadja C.
AU - Ragusa, Andrea
AU - Dorfs, Dirk
AU - Genovese, Alessandro
AU - Marotta, Roberto
AU - Manna, Liberato
AU - Pellegrino, Teresa
PY - 2011/1/10
Y1 - 2011/1/10
N2 - Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic-fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).
AB - Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic-fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).
KW - cell sorting
KW - magnetic clusters
KW - magnetic nanoparticles
KW - magnetic-fluorescent nanostructures
KW - quantumdots
KW - tumor labeling
UR - http://www.scopus.com/inward/record.url?scp=79951886844&partnerID=8YFLogxK
U2 - 10.1021/nn102761t
DO - 10.1021/nn102761t
M3 - Article
C2 - 21218823
AN - SCOPUS:79951886844
VL - 5
SP - 1109
EP - 1121
JO - ACS NANO
JF - ACS NANO
SN - 1936-0851
IS - 2
ER -