Multifunctional Nanobeads Based on Quantum Dots and Magnetic Nanoparticles: Synthesis and Cancer Cell Targeting and Sorting: Synthesis and cancer cell targeting and sorting

Research output: Contribution to journalArticleResearchpeer review

Authors

  • Riccardo Di Corato
  • Nadja C. Bigall
  • Andrea Ragusa
  • Dirk Dorfs
  • Alessandro Genovese
  • Roberto Marotta
  • Liberato Manna
  • Teresa Pellegrino

External Research Organisations

  • National Research Council Italy (CNR)
  • Center for Nanotechnology Innovation, Pisa
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Details

Original languageEnglish
Pages (from-to)1109-1121
Number of pages13
JournalACS NANO
Volume5
Issue number2
Publication statusPublished - 10 Jan 2011
Externally publishedYes

Abstract

Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic-fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).

Keywords

    cell sorting, magnetic clusters, magnetic nanoparticles, magnetic-fluorescent nanostructures, quantumdots, tumor labeling

ASJC Scopus subject areas

Sustainable Development Goals

Cite this

Multifunctional Nanobeads Based on Quantum Dots and Magnetic Nanoparticles: Synthesis and Cancer Cell Targeting and Sorting: Synthesis and cancer cell targeting and sorting. / Di Corato, Riccardo; Bigall, Nadja C.; Ragusa, Andrea et al.
In: ACS NANO, Vol. 5, No. 2, 10.01.2011, p. 1109-1121.

Research output: Contribution to journalArticleResearchpeer review

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abstract = "Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic-fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).",
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