Details
| Original language | English |
|---|---|
| Pages (from-to) | 9-12 |
| Number of pages | 4 |
| Journal | Propagation of Ornamental Plants |
| Volume | 8 |
| Issue number | 1 |
| Publication status | Published - Mar 2008 |
Abstract
A protoplast to plant regeneration system will open the way for somatic hybridization in cyclamen (Cyclamen persicum Mill.). A protocol for efficient plant regeneration from protoplasts isolated from embryogenic suspensions has been developed in cyclamen for the first time, and then successfully applied to different genotypes. Enzymatic digestion in 2.0% cellulose R10 and 0.5% macerozyme R10 resulted in yields between 1.0 and 5.0 × 105 protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. Cell division was recorded for all eleven tested genotypes in frequencies of up to 12% and 18% after seven and 14 days, respectively. Plant regeneration via somatic embryogenesis succeeded in nine out of the 11 genotypes under investigation. Protoplast-derived plants from eight genotypes have been successfully transferred to soil. Somaclonal variation was detected in terms of ploidy levels of regenerants, malformed flowers, and formation of multiple vegetative meristems with delayed flowering. However, its degree depended on the genotype and the time period between isolation of protoplasts and plant regeneration.
Keywords
- Genotypic differences, Ornamental plant, Protoplast culture, Somaclonal variation, Somatic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Forestry
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Propagation of Ornamental Plants, Vol. 8, No. 1, 03.2008, p. 9-12.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Morphological characterization of plants regenerated from PROTOPLASTS of Cyclamen persicum Mill
AU - Winkelmann, Traud
AU - Sabine Prange, Anika Nadja
AU - Specht, Janine
AU - Serek, Margrethe
PY - 2008/3
Y1 - 2008/3
N2 - A protoplast to plant regeneration system will open the way for somatic hybridization in cyclamen (Cyclamen persicum Mill.). A protocol for efficient plant regeneration from protoplasts isolated from embryogenic suspensions has been developed in cyclamen for the first time, and then successfully applied to different genotypes. Enzymatic digestion in 2.0% cellulose R10 and 0.5% macerozyme R10 resulted in yields between 1.0 and 5.0 × 105 protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. Cell division was recorded for all eleven tested genotypes in frequencies of up to 12% and 18% after seven and 14 days, respectively. Plant regeneration via somatic embryogenesis succeeded in nine out of the 11 genotypes under investigation. Protoplast-derived plants from eight genotypes have been successfully transferred to soil. Somaclonal variation was detected in terms of ploidy levels of regenerants, malformed flowers, and formation of multiple vegetative meristems with delayed flowering. However, its degree depended on the genotype and the time period between isolation of protoplasts and plant regeneration.
AB - A protoplast to plant regeneration system will open the way for somatic hybridization in cyclamen (Cyclamen persicum Mill.). A protocol for efficient plant regeneration from protoplasts isolated from embryogenic suspensions has been developed in cyclamen for the first time, and then successfully applied to different genotypes. Enzymatic digestion in 2.0% cellulose R10 and 0.5% macerozyme R10 resulted in yields between 1.0 and 5.0 × 105 protoplasts per gram fresh mass depending on the genotype. Protoplasts were immobilized in alginate films, which promoted proper cell wall regeneration. Cell division was recorded for all eleven tested genotypes in frequencies of up to 12% and 18% after seven and 14 days, respectively. Plant regeneration via somatic embryogenesis succeeded in nine out of the 11 genotypes under investigation. Protoplast-derived plants from eight genotypes have been successfully transferred to soil. Somaclonal variation was detected in terms of ploidy levels of regenerants, malformed flowers, and formation of multiple vegetative meristems with delayed flowering. However, its degree depended on the genotype and the time period between isolation of protoplasts and plant regeneration.
KW - Genotypic differences
KW - Ornamental plant
KW - Protoplast culture
KW - Somaclonal variation
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=39449104490&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:39449104490
VL - 8
SP - 9
EP - 12
JO - Propagation of Ornamental Plants
JF - Propagation of Ornamental Plants
SN - 1311-9109
IS - 1
ER -