Details
| Original language | English |
|---|---|
| Pages (from-to) | 665-675 |
| Number of pages | 11 |
| Journal | Journal of phytopathology |
| Volume | 159 |
| Issue number | 10 |
| Publication status | Published - 5 Sept 2011 |
Abstract
Hellebore leaf spot, caused by Coniothyrium hellebori, is the most common fungal disease of Helleborus species not only in botanical and ornamental gardens but also in nurseries. To correct the current lack of knowledge regarding this widely distributed pathogen, this study investigated 25 C. hellebori isolates collected from different countries in North America and Europe, primarily Germany. The morphology, pathogenicity and molecular genetic relationships on the basis of random amplified polymorphic DNA (RAPD) of these isolates were studied. RAPD primers produced a total of 394 bands, of which 40% were polymorphic. Genetic distances were calculated, and a dendrogram with bootstrap analysis was constructed by the unweighted pair group method with arithmetic mean (UPGMA) cluster method. All isolates were identified as C. hellebori, the causal agent of the disease. Two C. hellebori subclades were found, which could not be correlated with the geographic origin of the isolate, but with the plant host species and morphological characteristics. Sequence comparisons of the large subunit and internal transcribed spacer loci between C. hellebori and sequences from GenBank revealed that C. hellebori has to be grouped into the Didymellaceae family and rather belongs to Phoma or Microsphaeropsis than to Coniothyrium. This work represents the first study of this plant pathogen causing severe damage in Helleborus stocks and provides important information for the development of future Helleborus resistance breeding strategies.
Keywords
- Black spot, Coniothyrium hellebori, Fungal disease, Genetic variability, Helleborus, Microsphaeropsis, Phoma, Random amplified polymorphic DNA
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of phytopathology, Vol. 159, No. 10, 05.09.2011, p. 665-675.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Morphological and genetic analyses of hellebore leaf spot disease isolates from different geographic origins show low variability and reveal molecular evidence for reclassification into Didymellaceae
AU - Meiners, Julia
AU - Winkelmann, Traud
PY - 2011/9/5
Y1 - 2011/9/5
N2 - Hellebore leaf spot, caused by Coniothyrium hellebori, is the most common fungal disease of Helleborus species not only in botanical and ornamental gardens but also in nurseries. To correct the current lack of knowledge regarding this widely distributed pathogen, this study investigated 25 C. hellebori isolates collected from different countries in North America and Europe, primarily Germany. The morphology, pathogenicity and molecular genetic relationships on the basis of random amplified polymorphic DNA (RAPD) of these isolates were studied. RAPD primers produced a total of 394 bands, of which 40% were polymorphic. Genetic distances were calculated, and a dendrogram with bootstrap analysis was constructed by the unweighted pair group method with arithmetic mean (UPGMA) cluster method. All isolates were identified as C. hellebori, the causal agent of the disease. Two C. hellebori subclades were found, which could not be correlated with the geographic origin of the isolate, but with the plant host species and morphological characteristics. Sequence comparisons of the large subunit and internal transcribed spacer loci between C. hellebori and sequences from GenBank revealed that C. hellebori has to be grouped into the Didymellaceae family and rather belongs to Phoma or Microsphaeropsis than to Coniothyrium. This work represents the first study of this plant pathogen causing severe damage in Helleborus stocks and provides important information for the development of future Helleborus resistance breeding strategies.
AB - Hellebore leaf spot, caused by Coniothyrium hellebori, is the most common fungal disease of Helleborus species not only in botanical and ornamental gardens but also in nurseries. To correct the current lack of knowledge regarding this widely distributed pathogen, this study investigated 25 C. hellebori isolates collected from different countries in North America and Europe, primarily Germany. The morphology, pathogenicity and molecular genetic relationships on the basis of random amplified polymorphic DNA (RAPD) of these isolates were studied. RAPD primers produced a total of 394 bands, of which 40% were polymorphic. Genetic distances were calculated, and a dendrogram with bootstrap analysis was constructed by the unweighted pair group method with arithmetic mean (UPGMA) cluster method. All isolates were identified as C. hellebori, the causal agent of the disease. Two C. hellebori subclades were found, which could not be correlated with the geographic origin of the isolate, but with the plant host species and morphological characteristics. Sequence comparisons of the large subunit and internal transcribed spacer loci between C. hellebori and sequences from GenBank revealed that C. hellebori has to be grouped into the Didymellaceae family and rather belongs to Phoma or Microsphaeropsis than to Coniothyrium. This work represents the first study of this plant pathogen causing severe damage in Helleborus stocks and provides important information for the development of future Helleborus resistance breeding strategies.
KW - Black spot
KW - Coniothyrium hellebori
KW - Fungal disease
KW - Genetic variability
KW - Helleborus
KW - Microsphaeropsis
KW - Phoma
KW - Random amplified polymorphic DNA
UR - http://www.scopus.com/inward/record.url?scp=80052439296&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0434.2011.01823.x
DO - 10.1111/j.1439-0434.2011.01823.x
M3 - Article
AN - SCOPUS:80052439296
VL - 159
SP - 665
EP - 675
JO - Journal of phytopathology
JF - Journal of phytopathology
SN - 0931-1785
IS - 10
ER -