Details
| Original language | English |
|---|---|
| Pages (from-to) | B7-B12 |
| Journal | The Chemical Engineering Journal |
| Volume | 34 |
| Issue number | 1 |
| Publication status | Published - Jan 1987 |
Abstract
NADH-dependent culture fluorescence was monitored during various E. coli cultivation processes (batch and continuous cultivation in stirred tank reactors or bubble column reactors). Various strains of E. coli (including genetically modified E. coli) were used. Measurement of the fluorescence signal allows biomass estimation under certain conditions. The monitoring of culture fluorescence is useful for obtaining information on the metabolic status of the cells. The effect on the cells of different substrates (glucose and oxygen) was studied. The measurements were performed in synthetic and complex media and the influence of the composition of each medium was investigated. A microfluorometer was used as a routine sensor for cultivation control and provided insight into the status of the cells.
ASJC Scopus subject areas
- Engineering(all)
- General Engineering
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In: The Chemical Engineering Journal, Vol. 34, No. 1, 01.1987, p. B7-B12.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Monitoring of NADH-dependent culture fluorescence during the cultivation of Escherichia coli
AU - Scheper, Th
AU - Gebauer, A.
AU - Schügerl, K.
PY - 1987/1
Y1 - 1987/1
N2 - NADH-dependent culture fluorescence was monitored during various E. coli cultivation processes (batch and continuous cultivation in stirred tank reactors or bubble column reactors). Various strains of E. coli (including genetically modified E. coli) were used. Measurement of the fluorescence signal allows biomass estimation under certain conditions. The monitoring of culture fluorescence is useful for obtaining information on the metabolic status of the cells. The effect on the cells of different substrates (glucose and oxygen) was studied. The measurements were performed in synthetic and complex media and the influence of the composition of each medium was investigated. A microfluorometer was used as a routine sensor for cultivation control and provided insight into the status of the cells.
AB - NADH-dependent culture fluorescence was monitored during various E. coli cultivation processes (batch and continuous cultivation in stirred tank reactors or bubble column reactors). Various strains of E. coli (including genetically modified E. coli) were used. Measurement of the fluorescence signal allows biomass estimation under certain conditions. The monitoring of culture fluorescence is useful for obtaining information on the metabolic status of the cells. The effect on the cells of different substrates (glucose and oxygen) was studied. The measurements were performed in synthetic and complex media and the influence of the composition of each medium was investigated. A microfluorometer was used as a routine sensor for cultivation control and provided insight into the status of the cells.
UR - http://www.scopus.com/inward/record.url?scp=0023108617&partnerID=8YFLogxK
U2 - 10.1016/0300-9467(87)85009-8
DO - 10.1016/0300-9467(87)85009-8
M3 - Article
AN - SCOPUS:0023108617
VL - 34
SP - B7-B12
JO - The Chemical Engineering Journal
JF - The Chemical Engineering Journal
SN - 0300-9467
IS - 1
ER -