Details
| Original language | English |
|---|---|
| Pages (from-to) | 253-257 |
| Number of pages | 5 |
| Journal | Algal Research |
| Volume | 2 |
| Issue number | 3 |
| Publication status | Published - 26 Apr 2013 |
Abstract
Microalgal cultivations present challenges for monitoring and process control posed by their large scale and the likelihood that they will be composed of multiple species. Cell concentration is a fundamental parameter in any cultivation but is typically measured using off-line methods that may be time-consuming, laborious, or subject to interferences. Here, an in-situ microscope has been adapted for monitoring microalgal cultivations by adding a flow-through cell and adjusting image-processing algorithms. After installation in the bypass of a photobioreactor, the microscope enabled the continuous, automated acquisition of cell count, cell size, and cell morphology data on-line during cultivation processes over a period of 20. days, without sampling. The flow-through microscope was tested in cultivations of Chlamydomonas reinhardtii and Chlorella vulgaris. Cell concentration measurements were in agreement with off-line optical density measurements for both species. In addition, cell size and morphology distributions were obtained that revealed population shifts during the cultivation of C. vulgaris. This monitoring system thus provides a means to obtain detailed, non-invasive insights of microalgal cultivation processes.
Keywords
- Automated image processing, Cell count, Cell size distribution, Flow-through microscopy, Microalgae
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
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In: Algal Research, Vol. 2, No. 3, 26.04.2013, p. 253-257.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Monitoring of microalgal cultivations with on-line, flow-through microscopy
AU - Havlik, Ivo
AU - Reardon, Kenneth F.
AU - Ünal, Mehmet
AU - Lindner, Patrick
AU - Prediger, Andreas
AU - Babitzky, Alexander
AU - Beutel, Sascha
AU - Scheper, Thomas
N1 - Funding information: This work was supported by the Sustainable Bioenergy Development Center of Colorado State University and by funds from the Jud and Pat Harper Professorship in Chemical and Biological Engineering (to KFR).
PY - 2013/4/26
Y1 - 2013/4/26
N2 - Microalgal cultivations present challenges for monitoring and process control posed by their large scale and the likelihood that they will be composed of multiple species. Cell concentration is a fundamental parameter in any cultivation but is typically measured using off-line methods that may be time-consuming, laborious, or subject to interferences. Here, an in-situ microscope has been adapted for monitoring microalgal cultivations by adding a flow-through cell and adjusting image-processing algorithms. After installation in the bypass of a photobioreactor, the microscope enabled the continuous, automated acquisition of cell count, cell size, and cell morphology data on-line during cultivation processes over a period of 20. days, without sampling. The flow-through microscope was tested in cultivations of Chlamydomonas reinhardtii and Chlorella vulgaris. Cell concentration measurements were in agreement with off-line optical density measurements for both species. In addition, cell size and morphology distributions were obtained that revealed population shifts during the cultivation of C. vulgaris. This monitoring system thus provides a means to obtain detailed, non-invasive insights of microalgal cultivation processes.
AB - Microalgal cultivations present challenges for monitoring and process control posed by their large scale and the likelihood that they will be composed of multiple species. Cell concentration is a fundamental parameter in any cultivation but is typically measured using off-line methods that may be time-consuming, laborious, or subject to interferences. Here, an in-situ microscope has been adapted for monitoring microalgal cultivations by adding a flow-through cell and adjusting image-processing algorithms. After installation in the bypass of a photobioreactor, the microscope enabled the continuous, automated acquisition of cell count, cell size, and cell morphology data on-line during cultivation processes over a period of 20. days, without sampling. The flow-through microscope was tested in cultivations of Chlamydomonas reinhardtii and Chlorella vulgaris. Cell concentration measurements were in agreement with off-line optical density measurements for both species. In addition, cell size and morphology distributions were obtained that revealed population shifts during the cultivation of C. vulgaris. This monitoring system thus provides a means to obtain detailed, non-invasive insights of microalgal cultivation processes.
KW - Automated image processing
KW - Cell count
KW - Cell size distribution
KW - Flow-through microscopy
KW - Microalgae
UR - http://www.scopus.com/inward/record.url?scp=84879264283&partnerID=8YFLogxK
U2 - 10.15488/1320
DO - 10.15488/1320
M3 - Article
AN - SCOPUS:84879264283
VL - 2
SP - 253
EP - 257
JO - Algal Research
JF - Algal Research
SN - 2211-9264
IS - 3
ER -