Molecular identification of the ten subunits of cytochrome-c reductase from potato mitochondria

Research output: Contribution to journalArticleResearchpeer review

Authors

External Research Organisations

  • Max Planck Institute of Molecular Plant Physiology (MPI-MP)
  • Applied Biosystems GmbH
View graph of relations

Details

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalPlanta
Volume193
Issue number1
Publication statusPublished - Mar 1994
Externally publishedYes

Abstract

Cytochrome-c reductase (EC 1.10.2.2.) from Solanum tuberosum L. comprises ten subunits with apparent molecular sizes of 55, 53, 51, 35, 33, 25, 14, 12, 11 and 10 kDa on 14% SDS-PAGE. The identity of the subunits was analysed by direct amino-acid sequencing via cyclic Edman degradation. A large-scale purification procedure for the enzyme complex based on affinity chromatography and gelfiltraton is described. All subunits were enzymatically fragmented and the generated peptides were separated by reverse-phase HPLC. Complete or partial sequence determination of 33 peptides comprising a total of nearly 500 amino acids showed, that cytochrome-c reductase from potato contains three respiratory proteins (cytochrome b, cytochrome c1 and the "Rieske" iron-sulfur protein), four small proteins with molecular sizes below 15 kDa (so-called Q-binding, hinge, cytochrome-c1-linked and core-linked proteins) and three proteins in the 50-kDa range which show similarity to members of the core/PEP/MPP protein family (core/processing enhancing protein/mitochondrial processing peptidase). In fact these subunits show highest sequence identity either to MPP or PEP, which is in line with earlier findings, that isolated cytochrome-c reductase from potato exhibits processing activity towards mitochondrial precursor proteins.

Keywords

    Cytochrome-c reductase, Mitochondria, Mitochondrial processing peptidase, Respiratory chain, Solanum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Agricultural and Biological Sciences(all)
  • Plant Science

Cite this

Molecular identification of the ten subunits of cytochrome-c reductase from potato mitochondria. / Braun, Hans Peter; Kruft, Volker; Schmitz, Udo.
In: Planta, Vol. 193, No. 1, 03.1994, p. 99-106.

Research output: Contribution to journalArticleResearchpeer review

Braun HP, Kruft V, Schmitz U. Molecular identification of the ten subunits of cytochrome-c reductase from potato mitochondria. Planta. 1994 Mar;193(1):99-106. doi: 10.1007/BF00191612
Download
@article{4a4eecfe9dd3435cb7d3f85cdb95457d,
title = "Molecular identification of the ten subunits of cytochrome-c reductase from potato mitochondria",
abstract = "Cytochrome-c reductase (EC 1.10.2.2.) from Solanum tuberosum L. comprises ten subunits with apparent molecular sizes of 55, 53, 51, 35, 33, 25, 14, 12, 11 and 10 kDa on 14% SDS-PAGE. The identity of the subunits was analysed by direct amino-acid sequencing via cyclic Edman degradation. A large-scale purification procedure for the enzyme complex based on affinity chromatography and gelfiltraton is described. All subunits were enzymatically fragmented and the generated peptides were separated by reverse-phase HPLC. Complete or partial sequence determination of 33 peptides comprising a total of nearly 500 amino acids showed, that cytochrome-c reductase from potato contains three respiratory proteins (cytochrome b, cytochrome c1 and the {"}Rieske{"} iron-sulfur protein), four small proteins with molecular sizes below 15 kDa (so-called Q-binding, hinge, cytochrome-c1-linked and core-linked proteins) and three proteins in the 50-kDa range which show similarity to members of the core/PEP/MPP protein family (core/processing enhancing protein/mitochondrial processing peptidase). In fact these subunits show highest sequence identity either to MPP or PEP, which is in line with earlier findings, that isolated cytochrome-c reductase from potato exhibits processing activity towards mitochondrial precursor proteins.",
keywords = "Cytochrome-c reductase, Mitochondria, Mitochondrial processing peptidase, Respiratory chain, Solanum",
author = "Braun, {Hans Peter} and Volker Kruft and Udo Schmitz",
year = "1994",
month = mar,
doi = "10.1007/BF00191612",
language = "English",
volume = "193",
pages = "99--106",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "1",

}

Download

TY - JOUR

T1 - Molecular identification of the ten subunits of cytochrome-c reductase from potato mitochondria

AU - Braun, Hans Peter

AU - Kruft, Volker

AU - Schmitz, Udo

PY - 1994/3

Y1 - 1994/3

N2 - Cytochrome-c reductase (EC 1.10.2.2.) from Solanum tuberosum L. comprises ten subunits with apparent molecular sizes of 55, 53, 51, 35, 33, 25, 14, 12, 11 and 10 kDa on 14% SDS-PAGE. The identity of the subunits was analysed by direct amino-acid sequencing via cyclic Edman degradation. A large-scale purification procedure for the enzyme complex based on affinity chromatography and gelfiltraton is described. All subunits were enzymatically fragmented and the generated peptides were separated by reverse-phase HPLC. Complete or partial sequence determination of 33 peptides comprising a total of nearly 500 amino acids showed, that cytochrome-c reductase from potato contains three respiratory proteins (cytochrome b, cytochrome c1 and the "Rieske" iron-sulfur protein), four small proteins with molecular sizes below 15 kDa (so-called Q-binding, hinge, cytochrome-c1-linked and core-linked proteins) and three proteins in the 50-kDa range which show similarity to members of the core/PEP/MPP protein family (core/processing enhancing protein/mitochondrial processing peptidase). In fact these subunits show highest sequence identity either to MPP or PEP, which is in line with earlier findings, that isolated cytochrome-c reductase from potato exhibits processing activity towards mitochondrial precursor proteins.

AB - Cytochrome-c reductase (EC 1.10.2.2.) from Solanum tuberosum L. comprises ten subunits with apparent molecular sizes of 55, 53, 51, 35, 33, 25, 14, 12, 11 and 10 kDa on 14% SDS-PAGE. The identity of the subunits was analysed by direct amino-acid sequencing via cyclic Edman degradation. A large-scale purification procedure for the enzyme complex based on affinity chromatography and gelfiltraton is described. All subunits were enzymatically fragmented and the generated peptides were separated by reverse-phase HPLC. Complete or partial sequence determination of 33 peptides comprising a total of nearly 500 amino acids showed, that cytochrome-c reductase from potato contains three respiratory proteins (cytochrome b, cytochrome c1 and the "Rieske" iron-sulfur protein), four small proteins with molecular sizes below 15 kDa (so-called Q-binding, hinge, cytochrome-c1-linked and core-linked proteins) and three proteins in the 50-kDa range which show similarity to members of the core/PEP/MPP protein family (core/processing enhancing protein/mitochondrial processing peptidase). In fact these subunits show highest sequence identity either to MPP or PEP, which is in line with earlier findings, that isolated cytochrome-c reductase from potato exhibits processing activity towards mitochondrial precursor proteins.

KW - Cytochrome-c reductase

KW - Mitochondria

KW - Mitochondrial processing peptidase

KW - Respiratory chain

KW - Solanum

UR - http://www.scopus.com/inward/record.url?scp=0028052548&partnerID=8YFLogxK

U2 - 10.1007/BF00191612

DO - 10.1007/BF00191612

M3 - Article

C2 - 7764624

AN - SCOPUS:0028052548

VL - 193

SP - 99

EP - 106

JO - Planta

JF - Planta

SN - 0032-0935

IS - 1

ER -

By the same author(s)