Details
| Original language | English |
|---|---|
| Title of host publication | XXIV International Eucarpia Symposium Section Ornamentals |
| Subtitle of host publication | Ornamental Breeding Worldwide |
| Publisher | International Society for Horticultural Science |
| Pages | 261-268 |
| Number of pages | 8 |
| ISBN (print) | 9789066054172 |
| Publication status | Published - 1 Sept 2012 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 953 |
| ISSN (Print) | 0567-7572 |
Abstract
To clarify the molecular mechanism of flower development in Rosa hybrida L. different MADS-box-genes were investigated in normally developed flowers and in malformed flowers (similar C-function mutation). Expression levels of all A, B and C class genes were examined by qRT-PCR in different flower organs of both types of flowers. Expression patterns of MADS-box genes in early organ development were investigated by in-situ RT-PCR. Three different unknown APETALA1 homologous cDNA's were isolated and designated as RhAP1-1, RhAP1-2 and RhFUL. Exclusive expression of APETALA1 homologue genes RhAP1-1 and RhAP1-2 in whorl 1 and 2 of rose flowers demonstrate that the expression patterns are similar to APETALA1 homologue genes in other plant species. In contrast, RhFUL showed a unique expression pattern and was expressed only in sepals. Comparison of expression patterns between normal and malformed flowers demonstrated that two different B class genes were unaffected. All A class genes were up regulated and two C class genes were down regulated in all flower organs. Suppression of the C class genes RhC1 and RhC2 may be the reason for expression of RhAP1-1, RhAP1-2 and RhFUL in whorls 3 and 4 that lead to the malformed flower phenotype. Possible reasons for suppression of class C genes in early bud stages are discussed.
Keywords
- APETALA1, Class A, Flower development, MADS-box, Rosa × hybrida
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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XXIV International Eucarpia Symposium Section Ornamentals: Ornamental Breeding Worldwide. International Society for Horticultural Science, 2012. p. 261-268 (Acta Horticulturae; Vol. 953).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Molecular genetic investigation of malformed flower types of Rosa hybrida L.
AU - Mibus, H.
AU - Jedrzejuk, A.
AU - Serek, M.
PY - 2012/9/1
Y1 - 2012/9/1
N2 - To clarify the molecular mechanism of flower development in Rosa hybrida L. different MADS-box-genes were investigated in normally developed flowers and in malformed flowers (similar C-function mutation). Expression levels of all A, B and C class genes were examined by qRT-PCR in different flower organs of both types of flowers. Expression patterns of MADS-box genes in early organ development were investigated by in-situ RT-PCR. Three different unknown APETALA1 homologous cDNA's were isolated and designated as RhAP1-1, RhAP1-2 and RhFUL. Exclusive expression of APETALA1 homologue genes RhAP1-1 and RhAP1-2 in whorl 1 and 2 of rose flowers demonstrate that the expression patterns are similar to APETALA1 homologue genes in other plant species. In contrast, RhFUL showed a unique expression pattern and was expressed only in sepals. Comparison of expression patterns between normal and malformed flowers demonstrated that two different B class genes were unaffected. All A class genes were up regulated and two C class genes were down regulated in all flower organs. Suppression of the C class genes RhC1 and RhC2 may be the reason for expression of RhAP1-1, RhAP1-2 and RhFUL in whorls 3 and 4 that lead to the malformed flower phenotype. Possible reasons for suppression of class C genes in early bud stages are discussed.
AB - To clarify the molecular mechanism of flower development in Rosa hybrida L. different MADS-box-genes were investigated in normally developed flowers and in malformed flowers (similar C-function mutation). Expression levels of all A, B and C class genes were examined by qRT-PCR in different flower organs of both types of flowers. Expression patterns of MADS-box genes in early organ development were investigated by in-situ RT-PCR. Three different unknown APETALA1 homologous cDNA's were isolated and designated as RhAP1-1, RhAP1-2 and RhFUL. Exclusive expression of APETALA1 homologue genes RhAP1-1 and RhAP1-2 in whorl 1 and 2 of rose flowers demonstrate that the expression patterns are similar to APETALA1 homologue genes in other plant species. In contrast, RhFUL showed a unique expression pattern and was expressed only in sepals. Comparison of expression patterns between normal and malformed flowers demonstrated that two different B class genes were unaffected. All A class genes were up regulated and two C class genes were down regulated in all flower organs. Suppression of the C class genes RhC1 and RhC2 may be the reason for expression of RhAP1-1, RhAP1-2 and RhFUL in whorls 3 and 4 that lead to the malformed flower phenotype. Possible reasons for suppression of class C genes in early bud stages are discussed.
KW - APETALA1
KW - Class A
KW - Flower development
KW - MADS-box
KW - Rosa × hybrida
UR - http://www.scopus.com/inward/record.url?scp=84869988609&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2012.953.36
DO - 10.17660/ActaHortic.2012.953.36
M3 - Contribution to book/anthology
AN - SCOPUS:84869988609
SN - 9789066054172
T3 - Acta Horticulturae
SP - 261
EP - 268
BT - XXIV International Eucarpia Symposium Section Ornamentals
PB - International Society for Horticultural Science
ER -