Details
| Original language | English |
|---|---|
| Title of host publication | XXV International EUCARPIA Symposium Section Ornamentals |
| Subtitle of host publication | Crossing Borders |
| Editors | J. Van Huylenbroeck, E. Dhooghe |
| Publisher | International Society for Horticultural Science |
| Pages | 29-34 |
| Number of pages | 6 |
| ISBN (electronic) | 9789462610781 |
| Publication status | Published - 28 Jun 2015 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 1087 |
| ISSN (Print) | 0567-7572 |
| ISSN (electronic) | 2406-6168 |
Abstract
One of the top targets of recent breeding programs in ornamental plants is focused on developing an alternative way to the chemical growth retardants application in production of plants with compact growth habit. We describe work on Petunia hybrida 'Famous Lilac Dark Vein' plants with overexpreßed MAP kinase 4 nuclear substrate 1 (MKS1) and gibberellin 2-oxidase (GA2ox) genes. Petunia plants were transformed with pCAMBIA modified vectors in separate experiments, containing constructs either with the MKS1 or the GA2ox gene under the control of Ca35S promoter. The transformation was performed with the use of Agrobacterium tumefaciens strain GV3101. The integration and expreßion of the transgene was investigated using Southern blot and RT-PCR analysis. The mean internode lengths of the MKS1-transgenic lines were 2.5-fold shorter than those of the wild-type plants. The flowering delay in MKS1-transgenic lines was observed with a range of 6 to 11 days in comparison to the wild-type plants. The mean internode lengths of the GA2ox-transgenic lines were 6.5-fold shorter than those of the wild-type plants. The flowering delay in GA2ox-transgenic lines was observed with a range of 7 to 12 days in comparison to the wild-type plants. The flower morphology either in MKS1- or GA2ox-transgenic lines did not differ from wild-type plants. Constitutive overexpreßion of both MKS1 and GA2ox genes results in compact phenotype but also lead to delayed flowering.
Keywords
- Compact growth, Dwarfed phenotype, Gibberellin 2-oxidase, Growth retardation, MAP kinase substrate 1
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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XXV International EUCARPIA Symposium Section Ornamentals: Crossing Borders. ed. / J. Van Huylenbroeck; E. Dhooghe. International Society for Horticultural Science, 2015. p. 29-34 (Acta Horticulturae; Vol. 1087).
Research output: Chapter in book/report/conference proceeding › Contribution to book/anthology › Research › peer review
}
TY - CHAP
T1 - Molecular breeding of petunia hybrida 'famous lilac dark vein' for compact growth induction by MKS1 and GA2ox constitutive overexpreßion
AU - Gargul, J. M.
AU - Mibus, H.
AU - Serek, M.
PY - 2015/6/28
Y1 - 2015/6/28
N2 - One of the top targets of recent breeding programs in ornamental plants is focused on developing an alternative way to the chemical growth retardants application in production of plants with compact growth habit. We describe work on Petunia hybrida 'Famous Lilac Dark Vein' plants with overexpreßed MAP kinase 4 nuclear substrate 1 (MKS1) and gibberellin 2-oxidase (GA2ox) genes. Petunia plants were transformed with pCAMBIA modified vectors in separate experiments, containing constructs either with the MKS1 or the GA2ox gene under the control of Ca35S promoter. The transformation was performed with the use of Agrobacterium tumefaciens strain GV3101. The integration and expreßion of the transgene was investigated using Southern blot and RT-PCR analysis. The mean internode lengths of the MKS1-transgenic lines were 2.5-fold shorter than those of the wild-type plants. The flowering delay in MKS1-transgenic lines was observed with a range of 6 to 11 days in comparison to the wild-type plants. The mean internode lengths of the GA2ox-transgenic lines were 6.5-fold shorter than those of the wild-type plants. The flowering delay in GA2ox-transgenic lines was observed with a range of 7 to 12 days in comparison to the wild-type plants. The flower morphology either in MKS1- or GA2ox-transgenic lines did not differ from wild-type plants. Constitutive overexpreßion of both MKS1 and GA2ox genes results in compact phenotype but also lead to delayed flowering.
AB - One of the top targets of recent breeding programs in ornamental plants is focused on developing an alternative way to the chemical growth retardants application in production of plants with compact growth habit. We describe work on Petunia hybrida 'Famous Lilac Dark Vein' plants with overexpreßed MAP kinase 4 nuclear substrate 1 (MKS1) and gibberellin 2-oxidase (GA2ox) genes. Petunia plants were transformed with pCAMBIA modified vectors in separate experiments, containing constructs either with the MKS1 or the GA2ox gene under the control of Ca35S promoter. The transformation was performed with the use of Agrobacterium tumefaciens strain GV3101. The integration and expreßion of the transgene was investigated using Southern blot and RT-PCR analysis. The mean internode lengths of the MKS1-transgenic lines were 2.5-fold shorter than those of the wild-type plants. The flowering delay in MKS1-transgenic lines was observed with a range of 6 to 11 days in comparison to the wild-type plants. The mean internode lengths of the GA2ox-transgenic lines were 6.5-fold shorter than those of the wild-type plants. The flowering delay in GA2ox-transgenic lines was observed with a range of 7 to 12 days in comparison to the wild-type plants. The flower morphology either in MKS1- or GA2ox-transgenic lines did not differ from wild-type plants. Constitutive overexpreßion of both MKS1 and GA2ox genes results in compact phenotype but also lead to delayed flowering.
KW - Compact growth
KW - Dwarfed phenotype
KW - Gibberellin 2-oxidase
KW - Growth retardation
KW - MAP kinase substrate 1
UR - http://www.scopus.com/inward/record.url?scp=84940030644&partnerID=8YFLogxK
U2 - 10.17660/actahortic.2015.1087.2
DO - 10.17660/actahortic.2015.1087.2
M3 - Contribution to book/anthology
AN - SCOPUS:84940030644
T3 - Acta Horticulturae
SP - 29
EP - 34
BT - XXV International EUCARPIA Symposium Section Ornamentals
A2 - Van Huylenbroeck, J.
A2 - Dhooghe, E.
PB - International Society for Horticultural Science
ER -