Details
| Original language | English |
|---|---|
| Title of host publication | I International Symposium on Ornamentals in Africa |
| Editors | J.M. Wesonga, A.M. Opiyo |
| Publisher | International Society for Horticultural Science |
| Pages | 165-172 |
| Number of pages | 8 |
| ISBN (electronic) | 9789462610705 |
| Publication status | Published - 26 Mar 2015 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 1077 |
| ISSN (Print) | 0567-7572 |
Abstract
The development of an alternative method to chemical treatment for growth retardation in crop and ornamental plant production has become a top target in recent breeding programs. The present work describes the phenotypes of transgenic Kalanchoë blossfeldiana plants with overexpressed gibberellin 2-oxidase (GA2ox) from Nicotiana tabacum under the 35S promoter. The height of the transgenic Kalanchoë lines was reduced by a factor of almost 2 in comparison to the control plants, while the number of nodes was similar between the transgenic and control plants. The height of the inflorescence stem of the transgenic lines was approximately three times reduced. However, the transgenic clones exhibited a delay in flowering that amounted to approximately 24 days. The flower morphology for all of the investigated transgenic lines was the same as that for the control plants. The transgenic lines had visibly darker leaves containing approximately two times as much chlorophyll as the leaves of the control plants. These results demonstrate that the overexpression of GA2ox may become a useful method for obtaining compact growth of horticultural species without the use of chemical growth retardants.
Keywords
- Compact growth, Dwarf phenotype, GAox, Gibberellin 2-oxidase
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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I International Symposium on Ornamentals in Africa. ed. / J.M. Wesonga; A.M. Opiyo. International Society for Horticultural Science, 2015. p. 165-172 (Acta Horticulturae; Vol. 1077).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Molecular approach to plant growth regulation by constitutive overexpression of Nicotiana GA2ox in Kalanchoë blossfeldiana
AU - Gargul, J. M.
AU - Mibus, H.
AU - Serek, M.
N1 - Funding Information: The authors would like to thank Prof. em. Bjarne M. Stummann, the University of Copenhagen, Denmark, for linguistic correction of the manuscript. The authors would like to thank the staff of the floriculture section for technical assistance. This project was supported by a PhD research grant from the German Academic Exchange Service (DAAD) (Ref.: 323, PKZ: A/06/07504), which is gratefully acknowledged.
PY - 2015/3/26
Y1 - 2015/3/26
N2 - The development of an alternative method to chemical treatment for growth retardation in crop and ornamental plant production has become a top target in recent breeding programs. The present work describes the phenotypes of transgenic Kalanchoë blossfeldiana plants with overexpressed gibberellin 2-oxidase (GA2ox) from Nicotiana tabacum under the 35S promoter. The height of the transgenic Kalanchoë lines was reduced by a factor of almost 2 in comparison to the control plants, while the number of nodes was similar between the transgenic and control plants. The height of the inflorescence stem of the transgenic lines was approximately three times reduced. However, the transgenic clones exhibited a delay in flowering that amounted to approximately 24 days. The flower morphology for all of the investigated transgenic lines was the same as that for the control plants. The transgenic lines had visibly darker leaves containing approximately two times as much chlorophyll as the leaves of the control plants. These results demonstrate that the overexpression of GA2ox may become a useful method for obtaining compact growth of horticultural species without the use of chemical growth retardants.
AB - The development of an alternative method to chemical treatment for growth retardation in crop and ornamental plant production has become a top target in recent breeding programs. The present work describes the phenotypes of transgenic Kalanchoë blossfeldiana plants with overexpressed gibberellin 2-oxidase (GA2ox) from Nicotiana tabacum under the 35S promoter. The height of the transgenic Kalanchoë lines was reduced by a factor of almost 2 in comparison to the control plants, while the number of nodes was similar between the transgenic and control plants. The height of the inflorescence stem of the transgenic lines was approximately three times reduced. However, the transgenic clones exhibited a delay in flowering that amounted to approximately 24 days. The flower morphology for all of the investigated transgenic lines was the same as that for the control plants. The transgenic lines had visibly darker leaves containing approximately two times as much chlorophyll as the leaves of the control plants. These results demonstrate that the overexpression of GA2ox may become a useful method for obtaining compact growth of horticultural species without the use of chemical growth retardants.
KW - Compact growth
KW - Dwarf phenotype
KW - GAox
KW - Gibberellin 2-oxidase
UR - http://www.scopus.com/inward/record.url?scp=84983035835&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2015.1077.17
DO - 10.17660/ActaHortic.2015.1077.17
M3 - Conference contribution
AN - SCOPUS:84983035835
T3 - Acta Horticulturae
SP - 165
EP - 172
BT - I International Symposium on Ornamentals in Africa
A2 - Wesonga, J.M.
A2 - Opiyo, A.M.
PB - International Society for Horticultural Science
ER -