Details
| Original language | English |
|---|---|
| Pages (from-to) | 411-417 |
| Number of pages | 7 |
| Journal | Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz |
| Volume | 106 |
| Issue number | 4 |
| Publication status | Published - Jul 1999 |
Abstract
The C-terminal part of the RNA of Cocksfoot streak potyvirus (CSV) belonging to the Potyviridae was analyzed. CSV is transmissible by aphids and has a narrow host range in the Gramineae. The cloned cDNA fragments represent a single open reading frame (ORF) followed by a '''-non coding region and a poly(A)tail. By comparison with other potyviruses, the ORF includes the CP, the NIb and the NIa gene of CSV. An alignment of the ORF of CSV to the corresponding region of several potyviruses like PPV, PVY, TEV and TVMV shows about 50% identity. In contrast, the comparison of CSV to mite-transmitted as well as fungus-transmitted viruses shows only an identity of 30% and 23%, respectively. This reflects a low homology between CSV and mite- and fungus-transmitted Potyviridae infecting Gramineae. Alignments of the C-terminal potyviral proteins (NIa, NIb, CP) show more homology between CSV and potyviruses than between CSV and the other genera of the Potyviridae. Specific motifs, described for potyviral polyproteins, are all present in the polyprotein of CSV, too. The motif for aphid-transmission in the coat protein is found as well as the polymerase motif in the NIb-protein and the protease motif in the NIa-Pro protein. So far, these results indicate that a potyvirus infecting monocots (Gramineae) is quite similar to potyviruses infecting dicots.
Keywords
- Aphid transmission, Cocksfoot streak potyvirus, Monocotyledonous hosts, Potyviral proteins, Potyviridae
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, Vol. 106, No. 4, 07.1999, p. 411-417.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Molecular analyses of the genome of Cocksfoot streak potyvirus
AU - Götz, R.
AU - Huth, W.
AU - Maiss, E.
PY - 1999/7
Y1 - 1999/7
N2 - The C-terminal part of the RNA of Cocksfoot streak potyvirus (CSV) belonging to the Potyviridae was analyzed. CSV is transmissible by aphids and has a narrow host range in the Gramineae. The cloned cDNA fragments represent a single open reading frame (ORF) followed by a '''-non coding region and a poly(A)tail. By comparison with other potyviruses, the ORF includes the CP, the NIb and the NIa gene of CSV. An alignment of the ORF of CSV to the corresponding region of several potyviruses like PPV, PVY, TEV and TVMV shows about 50% identity. In contrast, the comparison of CSV to mite-transmitted as well as fungus-transmitted viruses shows only an identity of 30% and 23%, respectively. This reflects a low homology between CSV and mite- and fungus-transmitted Potyviridae infecting Gramineae. Alignments of the C-terminal potyviral proteins (NIa, NIb, CP) show more homology between CSV and potyviruses than between CSV and the other genera of the Potyviridae. Specific motifs, described for potyviral polyproteins, are all present in the polyprotein of CSV, too. The motif for aphid-transmission in the coat protein is found as well as the polymerase motif in the NIb-protein and the protease motif in the NIa-Pro protein. So far, these results indicate that a potyvirus infecting monocots (Gramineae) is quite similar to potyviruses infecting dicots.
AB - The C-terminal part of the RNA of Cocksfoot streak potyvirus (CSV) belonging to the Potyviridae was analyzed. CSV is transmissible by aphids and has a narrow host range in the Gramineae. The cloned cDNA fragments represent a single open reading frame (ORF) followed by a '''-non coding region and a poly(A)tail. By comparison with other potyviruses, the ORF includes the CP, the NIb and the NIa gene of CSV. An alignment of the ORF of CSV to the corresponding region of several potyviruses like PPV, PVY, TEV and TVMV shows about 50% identity. In contrast, the comparison of CSV to mite-transmitted as well as fungus-transmitted viruses shows only an identity of 30% and 23%, respectively. This reflects a low homology between CSV and mite- and fungus-transmitted Potyviridae infecting Gramineae. Alignments of the C-terminal potyviral proteins (NIa, NIb, CP) show more homology between CSV and potyviruses than between CSV and the other genera of the Potyviridae. Specific motifs, described for potyviral polyproteins, are all present in the polyprotein of CSV, too. The motif for aphid-transmission in the coat protein is found as well as the polymerase motif in the NIb-protein and the protease motif in the NIa-Pro protein. So far, these results indicate that a potyvirus infecting monocots (Gramineae) is quite similar to potyviruses infecting dicots.
KW - Aphid transmission
KW - Cocksfoot streak potyvirus
KW - Monocotyledonous hosts
KW - Potyviral proteins
KW - Potyviridae
UR - http://www.scopus.com/inward/record.url?scp=0345466381&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0345466381
VL - 106
SP - 411
EP - 417
JO - Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
JF - Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
SN - 0340-8159
IS - 4
ER -