Details
| Original language | English |
|---|---|
| Pages (from-to) | 27-37 |
| Number of pages | 11 |
| Journal | Prostaglandins Leukotrienes and Essential Fatty Acids |
| Volume | 90 |
| Issue number | 2-3 |
| Publication status | Published - 15 Jan 2014 |
Abstract
Introduction: Long chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) such as EPA and DHA have been shown to possess beneficial health effects, and it is believed that many of their effects are mediated by their oxygenated products (oxylipins). Recently, we have shown that serum levels of several hydroxy, epoxy, and dihydroxy FAs are dependent on the individual status of the parent FAs in a cohort of normo- and hyperlipidemic subjects. So far, the effect of an increased dietary LC n-3 PUFA intake on hydroxy, epoxy, and dihydroxy FA levels has not been investigated in subjects with mild combined hyperlipidemia. Subjects and methods: In the present study, we compared oxylipin patterns of 10 hyperlipidemic (cholesterol >200. mg/dl; triglyceride >150. mg/ml) and 10 normolipidemic men in response to twelve weeks of LC n-3 PUFA intake (1.14. g DHA and 1.56. g EPA). Levels of 44 free hydroxy, epoxy and dihydroxy FAs were analyzed in serum by LC-MS. Additionally, oxylipin levels were compared with their parent PUFA levels in erythrocyte membranes; a biomarker for the individual PUFA status. Results: Differences in the oxylipin pattern between normo- and hyperlipidemic subjects were minor before and after treatment. In all subjects, levels of EPA-derived oxylipins (170-4800. pM) were considerably elevated after LC n-3 PUFA intake (150-1400%), the increase of DHA-derived oxylipins (360-3900. pM) was less pronounced (30-130%). The relative change of EPA in erythrocyte membranes is strongly correlated (r≥0.5; p<0.05) with the relative change of corresponding epoxy and dihydroxy FA serum levels. The effect on arachidonic acid (AA)-derived oxylipin levels (140-27,100. pM) was inconsistent. Discussion and conclusions: The dietary LC PUFA composition has a direct influence on the endogenous oxylipin profile, including several highly biological active EPA- and DHA-derived lipid mediators. The shift in oxylipin pattern appears to be dependent on the initial LC PUFA status particularly for EPA. The finding that also levels of other oxylipins derived from ALA, LA or AA are modified by LC n-3 PUFA intake might suggest that at least some of the effects of EPA and DHA could be mediated by a shift in the entire oxylipin profile.
Keywords
- Adult, Dietary Supplements, Eicosapentaenoic Acid/analysis, Erythrocyte Membrane/chemistry, Fatty Acids/analysis, Fatty Acids, Omega-3/pharmacology, Humans, Hyperlipidemias/blood, Lipids/blood, Middle Aged, Oxylipins/blood, Young Adult, Hyperlipidemia, PUFA, Eicosanoids, Arachidonic acid, EPA, DHA, Epoxides, Diols, Omega-3 fatty acids
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Prostaglandins Leukotrienes and Essential Fatty Acids, Vol. 90, No. 2-3, 15.01.2014, p. 27-37.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Modulation of blood oxylipin levels by long-chain omega-3 fatty acid supplementation in hyper- and normolipidemic men
AU - Schuchardt, Jan Philipp
AU - Schmidt, Simone
AU - Kressel, Gaby
AU - Willenberg, Ina
AU - Hammock, Bruce D
AU - Hahn, Andreas
AU - Schebb, Nils Helge
N1 - Funding information: This study was supported by a Grant of the German Federal Ministry of Education and Research (BMBF) to AH, a Marie Curie Career Integration Grant to NHS , a Kekulé Ph.D. fellowship of the Fonds der Chemischen Industry to IW and a grants from US National Institutes of Health (NIH), Enviromental Health (NIEHS, P42 ES004699 and R01 ES002710 ) and Diabetes and Digestive and Kidney Diseases ( NIDDK, U24 DK097154 ) and the West Coast Central Comprehensive Metabolomics Resource Core (WC3MRC) to BDH. BDH is a George and Judy Marcus Senior Fellow of the American Asthma Association. The provision of the fish oil supplement by Dr. Loges+Co. GmbH (Winsen, Germany) is kindly acknowledged. The authors are solely responsible for the design and conduct of the study, collection, management, analysis, and interpretation of the data, as well as preparation of the manuscript. We would like to thank the participants of the study who contributed their time to this project.
PY - 2014/1/15
Y1 - 2014/1/15
N2 - Introduction: Long chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) such as EPA and DHA have been shown to possess beneficial health effects, and it is believed that many of their effects are mediated by their oxygenated products (oxylipins). Recently, we have shown that serum levels of several hydroxy, epoxy, and dihydroxy FAs are dependent on the individual status of the parent FAs in a cohort of normo- and hyperlipidemic subjects. So far, the effect of an increased dietary LC n-3 PUFA intake on hydroxy, epoxy, and dihydroxy FA levels has not been investigated in subjects with mild combined hyperlipidemia. Subjects and methods: In the present study, we compared oxylipin patterns of 10 hyperlipidemic (cholesterol >200. mg/dl; triglyceride >150. mg/ml) and 10 normolipidemic men in response to twelve weeks of LC n-3 PUFA intake (1.14. g DHA and 1.56. g EPA). Levels of 44 free hydroxy, epoxy and dihydroxy FAs were analyzed in serum by LC-MS. Additionally, oxylipin levels were compared with their parent PUFA levels in erythrocyte membranes; a biomarker for the individual PUFA status. Results: Differences in the oxylipin pattern between normo- and hyperlipidemic subjects were minor before and after treatment. In all subjects, levels of EPA-derived oxylipins (170-4800. pM) were considerably elevated after LC n-3 PUFA intake (150-1400%), the increase of DHA-derived oxylipins (360-3900. pM) was less pronounced (30-130%). The relative change of EPA in erythrocyte membranes is strongly correlated (r≥0.5; p<0.05) with the relative change of corresponding epoxy and dihydroxy FA serum levels. The effect on arachidonic acid (AA)-derived oxylipin levels (140-27,100. pM) was inconsistent. Discussion and conclusions: The dietary LC PUFA composition has a direct influence on the endogenous oxylipin profile, including several highly biological active EPA- and DHA-derived lipid mediators. The shift in oxylipin pattern appears to be dependent on the initial LC PUFA status particularly for EPA. The finding that also levels of other oxylipins derived from ALA, LA or AA are modified by LC n-3 PUFA intake might suggest that at least some of the effects of EPA and DHA could be mediated by a shift in the entire oxylipin profile.
AB - Introduction: Long chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) such as EPA and DHA have been shown to possess beneficial health effects, and it is believed that many of their effects are mediated by their oxygenated products (oxylipins). Recently, we have shown that serum levels of several hydroxy, epoxy, and dihydroxy FAs are dependent on the individual status of the parent FAs in a cohort of normo- and hyperlipidemic subjects. So far, the effect of an increased dietary LC n-3 PUFA intake on hydroxy, epoxy, and dihydroxy FA levels has not been investigated in subjects with mild combined hyperlipidemia. Subjects and methods: In the present study, we compared oxylipin patterns of 10 hyperlipidemic (cholesterol >200. mg/dl; triglyceride >150. mg/ml) and 10 normolipidemic men in response to twelve weeks of LC n-3 PUFA intake (1.14. g DHA and 1.56. g EPA). Levels of 44 free hydroxy, epoxy and dihydroxy FAs were analyzed in serum by LC-MS. Additionally, oxylipin levels were compared with their parent PUFA levels in erythrocyte membranes; a biomarker for the individual PUFA status. Results: Differences in the oxylipin pattern between normo- and hyperlipidemic subjects were minor before and after treatment. In all subjects, levels of EPA-derived oxylipins (170-4800. pM) were considerably elevated after LC n-3 PUFA intake (150-1400%), the increase of DHA-derived oxylipins (360-3900. pM) was less pronounced (30-130%). The relative change of EPA in erythrocyte membranes is strongly correlated (r≥0.5; p<0.05) with the relative change of corresponding epoxy and dihydroxy FA serum levels. The effect on arachidonic acid (AA)-derived oxylipin levels (140-27,100. pM) was inconsistent. Discussion and conclusions: The dietary LC PUFA composition has a direct influence on the endogenous oxylipin profile, including several highly biological active EPA- and DHA-derived lipid mediators. The shift in oxylipin pattern appears to be dependent on the initial LC PUFA status particularly for EPA. The finding that also levels of other oxylipins derived from ALA, LA or AA are modified by LC n-3 PUFA intake might suggest that at least some of the effects of EPA and DHA could be mediated by a shift in the entire oxylipin profile.
KW - Adult
KW - Dietary Supplements
KW - Eicosapentaenoic Acid/analysis
KW - Erythrocyte Membrane/chemistry
KW - Fatty Acids/analysis
KW - Fatty Acids, Omega-3/pharmacology
KW - Humans
KW - Hyperlipidemias/blood
KW - Lipids/blood
KW - Middle Aged
KW - Oxylipins/blood
KW - Young Adult
KW - Hyperlipidemia
KW - PUFA
KW - Eicosanoids
KW - Arachidonic acid
KW - EPA
KW - DHA
KW - Epoxides
KW - Diols
KW - Omega-3 fatty acids
UR - http://www.scopus.com/inward/record.url?scp=84895907696&partnerID=8YFLogxK
U2 - 10.1016/j.plefa.2013.12.008
DO - 10.1016/j.plefa.2013.12.008
M3 - Article
C2 - 24411718
VL - 90
SP - 27
EP - 37
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
IS - 2-3
ER -