Details
| Original language | English |
|---|---|
| Pages (from-to) | 173-182 |
| Number of pages | 10 |
| Journal | Bioprocess Engineering |
| Volume | 9 |
| Issue number | 4 |
| Publication status | Published - Aug 1993 |
Abstract
The cell cycling model (CCM) for S. cerevisiae proposed earlier is modified and tested with our own experimental data. Although the original CCM was well verified in steady states and exponential growth with data available in literature, some discrepancies between model predictions and experiments were found for the dynamics of fed-batch culture. The redistribution pattern of the age distribution of daughter cells is suggested as cause of the model error. With an exponential type of redistribution, instead of the original linear one, the model behaviour in transients is improved. The modified model was verified with data of fraction of budding cells and cell number for five fed-batch cultivations. The model agreed well with the experimental data. The simulation results suggest that the cell cycling process indeed is essentially in a pseudo-steady state during fed-batch cultivation, as was assumed in the model. Due to the strong correlation between the quality of baker's yeast and the state of the population in the cell cycling process, the model was applied to optimize the feeding rate of a fedbatch process with consideration of final product quality. The optimal feeding was used succesfully in a laboratory experiment, which demonstrates the validity of the model.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Bioprocess Engineering, Vol. 9, No. 4, 08.1993, p. 173-182.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Modification and verification of the dynamic cell cycling model for baker's yeast
AU - Yuan, J. Q.
AU - Bellgardt, K. H.
AU - Deckwer, W. D.
AU - Jiang, W. S.
PY - 1993/8
Y1 - 1993/8
N2 - The cell cycling model (CCM) for S. cerevisiae proposed earlier is modified and tested with our own experimental data. Although the original CCM was well verified in steady states and exponential growth with data available in literature, some discrepancies between model predictions and experiments were found for the dynamics of fed-batch culture. The redistribution pattern of the age distribution of daughter cells is suggested as cause of the model error. With an exponential type of redistribution, instead of the original linear one, the model behaviour in transients is improved. The modified model was verified with data of fraction of budding cells and cell number for five fed-batch cultivations. The model agreed well with the experimental data. The simulation results suggest that the cell cycling process indeed is essentially in a pseudo-steady state during fed-batch cultivation, as was assumed in the model. Due to the strong correlation between the quality of baker's yeast and the state of the population in the cell cycling process, the model was applied to optimize the feeding rate of a fedbatch process with consideration of final product quality. The optimal feeding was used succesfully in a laboratory experiment, which demonstrates the validity of the model.
AB - The cell cycling model (CCM) for S. cerevisiae proposed earlier is modified and tested with our own experimental data. Although the original CCM was well verified in steady states and exponential growth with data available in literature, some discrepancies between model predictions and experiments were found for the dynamics of fed-batch culture. The redistribution pattern of the age distribution of daughter cells is suggested as cause of the model error. With an exponential type of redistribution, instead of the original linear one, the model behaviour in transients is improved. The modified model was verified with data of fraction of budding cells and cell number for five fed-batch cultivations. The model agreed well with the experimental data. The simulation results suggest that the cell cycling process indeed is essentially in a pseudo-steady state during fed-batch cultivation, as was assumed in the model. Due to the strong correlation between the quality of baker's yeast and the state of the population in the cell cycling process, the model was applied to optimize the feeding rate of a fedbatch process with consideration of final product quality. The optimal feeding was used succesfully in a laboratory experiment, which demonstrates the validity of the model.
UR - http://www.scopus.com/inward/record.url?scp=0027171641&partnerID=8YFLogxK
U2 - 10.1007/BF00389926
DO - 10.1007/BF00389926
M3 - Article
AN - SCOPUS:0027171641
VL - 9
SP - 173
EP - 182
JO - Bioprocess Engineering
JF - Bioprocess Engineering
SN - 0178-515X
IS - 4
ER -