Details
| Original language | English |
|---|---|
| Pages (from-to) | 165-9 |
| Number of pages | 5 |
| Journal | FEBS letters |
| Volume | 547 |
| Issue number | 1-3 |
| Publication status | Published - 17 Jul 2003 |
Abstract
The methanogenic and hyperthermophilic deep-sea archaeon Methanococcus jannaschii has three putative K+ channels, MVP (Mj0139), MjK1 (Mj0138.1) and MjK2 (Mj1357). The physiological function of these K+ channels was examined in a viability assay, using the Escherichia coli mutant LB2003 (kup1, DeltakdpABC5, DeltatrkA). While MjK2 expression had no effects on the potassium-dependent phenotype of LB2003, MVP and MjK1 complemented the deficiency at a concentration of 1 mM KCl. In contrast to KcsA, MthK and MVP, MjK1 strongly affected host cell viability at 10 and 100 mM KCl. The toxic effects were less pronounced when growth media were supplemented with the K+ channel blocker BaCl2.
Keywords
- Amino Acid Sequence, Base Sequence, Cell Membrane/physiology, Cloning, Molecular, DNA Primers, Escherichia coli/genetics, Kinetics, Methanococcus/genetics, Molecular Sequence Data, Potassium/metabolism, Potassium Channels/chemistry, Recombinant Proteins/chemistry, Sequence Alignment, Sequence Homology, Amino Acid
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In: FEBS letters, Vol. 547, No. 1-3, 17.07.2003, p. 165-9.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - MjK1, a K+ channel from M. jannaschii, mediates K+ uptake and K+ sensitivity in E. coli
AU - Hellmer, Jens
AU - Zeilinger, Carsten
N1 - Funding information: We are grateful to Prof. E. Bakker for providing us with E. coli mutant LB2003. We thank M. Kieß (GBF, Braunschweig, Germany) for amino acid sequencing. We also thank Henri Tietge and Ramani Balasubramanian for proofreading. This project was supported by the FSP, Lower Saxony Agrar Biotechnology subproject CZ.
PY - 2003/7/17
Y1 - 2003/7/17
N2 - The methanogenic and hyperthermophilic deep-sea archaeon Methanococcus jannaschii has three putative K+ channels, MVP (Mj0139), MjK1 (Mj0138.1) and MjK2 (Mj1357). The physiological function of these K+ channels was examined in a viability assay, using the Escherichia coli mutant LB2003 (kup1, DeltakdpABC5, DeltatrkA). While MjK2 expression had no effects on the potassium-dependent phenotype of LB2003, MVP and MjK1 complemented the deficiency at a concentration of 1 mM KCl. In contrast to KcsA, MthK and MVP, MjK1 strongly affected host cell viability at 10 and 100 mM KCl. The toxic effects were less pronounced when growth media were supplemented with the K+ channel blocker BaCl2.
AB - The methanogenic and hyperthermophilic deep-sea archaeon Methanococcus jannaschii has three putative K+ channels, MVP (Mj0139), MjK1 (Mj0138.1) and MjK2 (Mj1357). The physiological function of these K+ channels was examined in a viability assay, using the Escherichia coli mutant LB2003 (kup1, DeltakdpABC5, DeltatrkA). While MjK2 expression had no effects on the potassium-dependent phenotype of LB2003, MVP and MjK1 complemented the deficiency at a concentration of 1 mM KCl. In contrast to KcsA, MthK and MVP, MjK1 strongly affected host cell viability at 10 and 100 mM KCl. The toxic effects were less pronounced when growth media were supplemented with the K+ channel blocker BaCl2.
KW - Amino Acid Sequence
KW - Base Sequence
KW - Cell Membrane/physiology
KW - Cloning, Molecular
KW - DNA Primers
KW - Escherichia coli/genetics
KW - Kinetics
KW - Methanococcus/genetics
KW - Molecular Sequence Data
KW - Potassium/metabolism
KW - Potassium Channels/chemistry
KW - Recombinant Proteins/chemistry
KW - Sequence Alignment
KW - Sequence Homology, Amino Acid
U2 - 10.1016/s0014-5793(03)00706-3
DO - 10.1016/s0014-5793(03)00706-3
M3 - Article
C2 - 12860407
VL - 547
SP - 165
EP - 169
JO - FEBS letters
JF - FEBS letters
SN - 0014-5793
IS - 1-3
ER -