Details
| Original language | English |
|---|---|
| Pages (from-to) | 1424-1426 |
| Number of pages | 3 |
| Journal | Molecular ecology resources |
| Volume | 9 |
| Issue number | 5 |
| Publication status | Published - Sept 2009 |
Abstract
We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.
Keywords
- Halophytes, Microsatellites, Spergularia media, SSRs
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
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In: Molecular ecology resources, Vol. 9, No. 5, 09.2009, p. 1424-1426.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Microsatellite markers for Spergularia media (L.) C. Presl. (Caryophyllaceae) and their cross-species transferability
AU - Prinz, Kathleen
AU - Schie, Stephan
AU - Debener, Thomas
AU - Hensen, Isabell
AU - Weising, Kurt
PY - 2009/9
Y1 - 2009/9
N2 - We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.
AB - We developed 11 polymorphic microsatellite markers for Spergularia media (Caryophyllaceae), a perennial halophyte of coastal salt meadows and continental areas of western Eurasia. The number of alleles per locus observed in a single population of 20 individuals from the German North Sea coast ranged from 3 to 20. Observed and expected heterozygosities ranged from 0.200 to 0.850 and from 0.278 to 0.936 respectively. Observed heterozygosities were lower than expected heterozygosities at all loci, presumably as a consequence of inbreeding. All markers cross-amplified in the closely related S. salina. Of these, nine were polymorphic in the heterologous species. Only two primer pairs generated PCR products in S. diandra.
KW - Halophytes
KW - Microsatellites
KW - Spergularia media
KW - SSRs
UR - http://www.scopus.com/inward/record.url?scp=68949112086&partnerID=8YFLogxK
U2 - 10.1111/j.1755-0998.2009.02680.x
DO - 10.1111/j.1755-0998.2009.02680.x
M3 - Article
AN - SCOPUS:68949112086
VL - 9
SP - 1424
EP - 1426
JO - Molecular ecology resources
JF - Molecular ecology resources
SN - 1755-098X
IS - 5
ER -