Details
| Original language | English |
|---|---|
| Pages (from-to) | 237-245 |
| Number of pages | 9 |
| Journal | Propagation of Ornamental Plants |
| Volume | 10 |
| Issue number | 4 |
| Publication status | Published - 2010 |
Abstract
The regeneration of Cyclamen persicum through somatic embryogenesis (SE) has been described by several groups for a wide spectrum of genotypes. Here, we studied this regeneration system for the first time with 30 genotypes which are valued as cut flowers. The aim of this study was to analyse the efficiency of the micropropagation through SE, to investigate the genetic fidelity and performance of the regenerants and to estimate production costs of young plants. The genotype significantly influenced the frequency of callus induction that ranged from 8 to 80% and also the frequency of calluses with differentiation of somatic embryos that ranged from 0 to 92%. Only three of the 30 genotypes were not able to regenerate somatic embryos, while plants were obtained from the remaining 27 genotypes. In total, 2,783 regenerants were transferred to the greenhouse, 2,003 (72%) of which could be acclimatized. Some genotypes were extremely uniform in terms of flower colour and flower shapes, whereas in others somaclonal variation was detected. Estimation of micropropagation cost was 2-3 € per acclimatized young plant at our research facility. If genotypes with high regeneration efficiencies are selected, plant production costs could be reduced to 0.56-0.80 € or even more.
Keywords
- Cyclamen, Genetic fidelity, In vitro propagation, Performance test, Production costs, Somaclonal variation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Forestry
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Propagation of Ornamental Plants, Vol. 10, No. 4, 2010, p. 237-245.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Micropropagation through somatic embryogenesis of Cyclamen persicum mill. Genotypes for cut flower production - feasibility study
AU - Winkelmann, Traud
AU - Ilczuk, Agnieszka
AU - Wartenberg, Stephan
PY - 2010
Y1 - 2010
N2 - The regeneration of Cyclamen persicum through somatic embryogenesis (SE) has been described by several groups for a wide spectrum of genotypes. Here, we studied this regeneration system for the first time with 30 genotypes which are valued as cut flowers. The aim of this study was to analyse the efficiency of the micropropagation through SE, to investigate the genetic fidelity and performance of the regenerants and to estimate production costs of young plants. The genotype significantly influenced the frequency of callus induction that ranged from 8 to 80% and also the frequency of calluses with differentiation of somatic embryos that ranged from 0 to 92%. Only three of the 30 genotypes were not able to regenerate somatic embryos, while plants were obtained from the remaining 27 genotypes. In total, 2,783 regenerants were transferred to the greenhouse, 2,003 (72%) of which could be acclimatized. Some genotypes were extremely uniform in terms of flower colour and flower shapes, whereas in others somaclonal variation was detected. Estimation of micropropagation cost was 2-3 € per acclimatized young plant at our research facility. If genotypes with high regeneration efficiencies are selected, plant production costs could be reduced to 0.56-0.80 € or even more.
AB - The regeneration of Cyclamen persicum through somatic embryogenesis (SE) has been described by several groups for a wide spectrum of genotypes. Here, we studied this regeneration system for the first time with 30 genotypes which are valued as cut flowers. The aim of this study was to analyse the efficiency of the micropropagation through SE, to investigate the genetic fidelity and performance of the regenerants and to estimate production costs of young plants. The genotype significantly influenced the frequency of callus induction that ranged from 8 to 80% and also the frequency of calluses with differentiation of somatic embryos that ranged from 0 to 92%. Only three of the 30 genotypes were not able to regenerate somatic embryos, while plants were obtained from the remaining 27 genotypes. In total, 2,783 regenerants were transferred to the greenhouse, 2,003 (72%) of which could be acclimatized. Some genotypes were extremely uniform in terms of flower colour and flower shapes, whereas in others somaclonal variation was detected. Estimation of micropropagation cost was 2-3 € per acclimatized young plant at our research facility. If genotypes with high regeneration efficiencies are selected, plant production costs could be reduced to 0.56-0.80 € or even more.
KW - Cyclamen
KW - Genetic fidelity
KW - In vitro propagation
KW - Performance test
KW - Production costs
KW - Somaclonal variation
UR - http://www.scopus.com/inward/record.url?scp=78650119319&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:78650119319
VL - 10
SP - 237
EP - 245
JO - Propagation of Ornamental Plants
JF - Propagation of Ornamental Plants
SN - 1311-9109
IS - 4
ER -