Details
| Original language | English |
|---|---|
| Pages (from-to) | 2681-2697 |
| Number of pages | 17 |
| Journal | Environmental microbiology |
| Volume | 25 |
| Issue number | 12 |
| Early online date | 12 Oct 2022 |
| Publication status | Published - 19 Dec 2023 |
Abstract
Environmental microplastic (MP) is ubiquitous in aquatic and terrestrial ecosystems providing artificial habitats for microbes. Mechanisms of MP colonization, MP polymer impacts, and effects on soil microbiomes are largely unknown in terrestrial systems. Therefore, we experimentally tested the hypothesis that MP polymer type is an important deterministic factor affecting MP community assembly by incubating common MP polymer types in situ in landfill soil for 14 months. 16S rRNA gene amplicon sequencing indicated that MP polymers have specific impacts on plastisphere microbiomes, which are subsets of the soil microbiome. Chloroflexota, Gammaproteobacteria, certain Nitrososphaerota, and Nanoarchaeota explained differences among MP polymers and time points. Plastisphere microbial community composition derived from different MP diverged over time and was enriched in potential pathogens. PICRUSt predictions of pathway abundances and quantitative PCR of functional marker genes indicated that MP polymers exerted an ambivalent effect on genetic potentials of biogeochemical cycles. Overall, the data indicates that (i) polymer type as deterministic factor rather than stochastic factors drives plastisphere community assembly, (ii) MP impacts greenhouse gas metabolism, xenobiotic degradation and pathogen distribution, and (iii) MP serves as an ideal model system for studying fundamental questions in microbial ecology such as community assembly mechanisms in terrestrial environments.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Immunology and Microbiology(all)
- Microbiology
Sustainable Development Goals
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In: Environmental microbiology, Vol. 25, No. 12, 19.12.2023, p. 2681-2697.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Microplastic polymer properties as deterministic factors driving terrestrial plastisphere microbiome assembly and succession in the field
AU - Rohrbach, Stephan
AU - Gkoutselis, Gerasimos
AU - Hink, Linda
AU - Weig, Alfons R
AU - Obst, Martin
AU - Diekmann, Astrid
AU - Ho, Adrian
AU - Rambold, Gerhard
AU - Horn, Marcus A
N1 - Funding Information: This study was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)—SFB 1357–391977956; subproject C04 and A02. Our gratitude is dedicated to Zweckverband Abfallwirtschaft Region Hannover, Germany, for the possibility to perform our field study at their property and for support of their team during sampling. The authors would also like to thank Peter Strohriegl and Lisa Weber (CRC 1357 Microplastic subproject Z01) for provision of microplastic particles and measurements of MP properties. Furthermore, we are thankful to Antonia Freiberger (BayCEER, Bayreuth; Germany) for her technical assistance during the CLSM. Anne Herwig and Leopold Sauheitl (Institute of Soil Science, Leibniz University Hannover, Germany) are acknowledged for technical support during the soil characterization. Open Access funding enabled and organized by Projekt DEAL.
PY - 2023/12/19
Y1 - 2023/12/19
N2 - Environmental microplastic (MP) is ubiquitous in aquatic and terrestrial ecosystems providing artificial habitats for microbes. Mechanisms of MP colonization, MP polymer impacts, and effects on soil microbiomes are largely unknown in terrestrial systems. Therefore, we experimentally tested the hypothesis that MP polymer type is an important deterministic factor affecting MP community assembly by incubating common MP polymer types in situ in landfill soil for 14 months. 16S rRNA gene amplicon sequencing indicated that MP polymers have specific impacts on plastisphere microbiomes, which are subsets of the soil microbiome. Chloroflexota, Gammaproteobacteria, certain Nitrososphaerota, and Nanoarchaeota explained differences among MP polymers and time points. Plastisphere microbial community composition derived from different MP diverged over time and was enriched in potential pathogens. PICRUSt predictions of pathway abundances and quantitative PCR of functional marker genes indicated that MP polymers exerted an ambivalent effect on genetic potentials of biogeochemical cycles. Overall, the data indicates that (i) polymer type as deterministic factor rather than stochastic factors drives plastisphere community assembly, (ii) MP impacts greenhouse gas metabolism, xenobiotic degradation and pathogen distribution, and (iii) MP serves as an ideal model system for studying fundamental questions in microbial ecology such as community assembly mechanisms in terrestrial environments.
AB - Environmental microplastic (MP) is ubiquitous in aquatic and terrestrial ecosystems providing artificial habitats for microbes. Mechanisms of MP colonization, MP polymer impacts, and effects on soil microbiomes are largely unknown in terrestrial systems. Therefore, we experimentally tested the hypothesis that MP polymer type is an important deterministic factor affecting MP community assembly by incubating common MP polymer types in situ in landfill soil for 14 months. 16S rRNA gene amplicon sequencing indicated that MP polymers have specific impacts on plastisphere microbiomes, which are subsets of the soil microbiome. Chloroflexota, Gammaproteobacteria, certain Nitrososphaerota, and Nanoarchaeota explained differences among MP polymers and time points. Plastisphere microbial community composition derived from different MP diverged over time and was enriched in potential pathogens. PICRUSt predictions of pathway abundances and quantitative PCR of functional marker genes indicated that MP polymers exerted an ambivalent effect on genetic potentials of biogeochemical cycles. Overall, the data indicates that (i) polymer type as deterministic factor rather than stochastic factors drives plastisphere community assembly, (ii) MP impacts greenhouse gas metabolism, xenobiotic degradation and pathogen distribution, and (iii) MP serves as an ideal model system for studying fundamental questions in microbial ecology such as community assembly mechanisms in terrestrial environments.
UR - http://www.scopus.com/inward/record.url?scp=85141367687&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.16234
DO - 10.1111/1462-2920.16234
M3 - Article
C2 - 36224114
VL - 25
SP - 2681
EP - 2697
JO - Environmental microbiology
JF - Environmental microbiology
SN - 1462-2912
IS - 12
ER -