Details
| Original language | English |
|---|---|
| Pages (from-to) | 966-973 |
| Number of pages | 8 |
| Journal | Burns |
| Volume | 40 |
| Issue number | 5 |
| Publication status | Published - Aug 2014 |
Abstract
The benefits and risks of singular and repetitive microneedling (1 mm) have not been thoroughly investigated. The aim of this study was to evaluate the benefits and risks of singular and repetitive skin needling with a microneedling device in an animal model with and without skincare. 30 Sprague Dawley rats were randomized to five groups: control, skin-care only (Vitamin A & C), 1× needling 1 mm, 4× needling 1 mm, 4× needling 1 mm with skin-care. All animals were euthanized after 10 weeks. Skin specimens were stained with HE and Masson's trichrome. Additionally, gene expression analysis with microarray technique for various growth factors (TGFβ1-3, FGF, EGF, VEGF, TNF-α) and real time reverse transcription PCR for collagen I & III were conducted. We showed that singular microneedling matches and repetitive microneedling sessions superposition epidermal and dermal benefits such as an increase of epidermal thickness (up to 658% increase, p value 0.0008) and dermal connective tissue--even more so when combined with skin-care with vitamin A and C. Juvenile collagen I showed itself up-regulated in all groups, while collagen III was down-regulated. Singular and repetitive PCI with a microneedling device can achieve and supersede the results already shown with medical needling.
Keywords
- Animals, Ascorbic Acid/therapeutic use, Cicatrix/genetics, Collagen Type I/genetics, Collagen Type III/genetics, Dermis/metabolism, Epidermis/metabolism, Needles, Rats, Sprague-Dawley, Regeneration/genetics, Reverse Transcriptase Polymerase Chain Reaction, Skin Care/methods, Transforming Growth Factor beta/genetics, Vitamin A/therapeutic use, Vitamins/therapeutic use, Medical needling, Percutaneous collagen induction (PCI), Burn scars, Microneedling, Collagen, Scar treatment, Epidermal thickening
ASJC Scopus subject areas
- Medicine(all)
- Critical Care and Intensive Care Medicine
- Medicine(all)
- Surgery
- Medicine(all)
- Emergency Medicine
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In: Burns, Vol. 40, No. 5, 08.2014, p. 966-973.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Microneedling
T2 - matching the results of medical needling and repetitive treatments to maximize potential for skin regeneration
AU - Zeitter, S
AU - Sikora, Z
AU - Jahn, S
AU - Stahl, F
AU - Strauß, S
AU - Lazaridis, A
AU - Reimers, K
AU - Vogt, P M
AU - Aust, M C
N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/8
Y1 - 2014/8
N2 - The benefits and risks of singular and repetitive microneedling (1 mm) have not been thoroughly investigated. The aim of this study was to evaluate the benefits and risks of singular and repetitive skin needling with a microneedling device in an animal model with and without skincare. 30 Sprague Dawley rats were randomized to five groups: control, skin-care only (Vitamin A & C), 1× needling 1 mm, 4× needling 1 mm, 4× needling 1 mm with skin-care. All animals were euthanized after 10 weeks. Skin specimens were stained with HE and Masson's trichrome. Additionally, gene expression analysis with microarray technique for various growth factors (TGFβ1-3, FGF, EGF, VEGF, TNF-α) and real time reverse transcription PCR for collagen I & III were conducted. We showed that singular microneedling matches and repetitive microneedling sessions superposition epidermal and dermal benefits such as an increase of epidermal thickness (up to 658% increase, p value 0.0008) and dermal connective tissue--even more so when combined with skin-care with vitamin A and C. Juvenile collagen I showed itself up-regulated in all groups, while collagen III was down-regulated. Singular and repetitive PCI with a microneedling device can achieve and supersede the results already shown with medical needling.
AB - The benefits and risks of singular and repetitive microneedling (1 mm) have not been thoroughly investigated. The aim of this study was to evaluate the benefits and risks of singular and repetitive skin needling with a microneedling device in an animal model with and without skincare. 30 Sprague Dawley rats were randomized to five groups: control, skin-care only (Vitamin A & C), 1× needling 1 mm, 4× needling 1 mm, 4× needling 1 mm with skin-care. All animals were euthanized after 10 weeks. Skin specimens were stained with HE and Masson's trichrome. Additionally, gene expression analysis with microarray technique for various growth factors (TGFβ1-3, FGF, EGF, VEGF, TNF-α) and real time reverse transcription PCR for collagen I & III were conducted. We showed that singular microneedling matches and repetitive microneedling sessions superposition epidermal and dermal benefits such as an increase of epidermal thickness (up to 658% increase, p value 0.0008) and dermal connective tissue--even more so when combined with skin-care with vitamin A and C. Juvenile collagen I showed itself up-regulated in all groups, while collagen III was down-regulated. Singular and repetitive PCI with a microneedling device can achieve and supersede the results already shown with medical needling.
KW - Animals
KW - Ascorbic Acid/therapeutic use
KW - Cicatrix/genetics
KW - Collagen Type I/genetics
KW - Collagen Type III/genetics
KW - Dermis/metabolism
KW - Epidermis/metabolism
KW - Needles
KW - Rats, Sprague-Dawley
KW - Regeneration/genetics
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Skin Care/methods
KW - Transforming Growth Factor beta/genetics
KW - Vitamin A/therapeutic use
KW - Vitamins/therapeutic use
KW - Medical needling
KW - Percutaneous collagen induction (PCI)
KW - Burn scars
KW - Microneedling
KW - Collagen
KW - Scar treatment
KW - Epidermal thickening
UR - http://www.scopus.com/inward/record.url?scp=84902551797&partnerID=8YFLogxK
U2 - 10.1016/j.burns.2013.12.008
DO - 10.1016/j.burns.2013.12.008
M3 - Article
C2 - 24513133
VL - 40
SP - 966
EP - 973
JO - Burns
JF - Burns
SN - 0305-4179
IS - 5
ER -