Details
| Original language | English |
|---|---|
| Pages (from-to) | 1105-1114 |
| Number of pages | 10 |
| Journal | Biotechnology journal |
| Volume | 8 |
| Issue number | 9 |
| Early online date | 7 Jun 2013 |
| Publication status | Published - 9 Sept 2013 |
Abstract
Escherichia coli is the most popular microorganism for the production of recombinant proteins and is gaining increasing importance for the production of low-molecular weight compounds such as amino acids. The metabolic cost associated with the production of amino acids and (recombinant) proteins from glucose, glycerol and acetate was determined using three different computational techniques to identify those amino acids that put the highest burden on the biosynthetic machinery of E. coli. Comparing the costs of individual amino acids, we find that methionine is the most expensive amino acid in terms of consumed mol of ATP per molecule produced, while leucine is the most expensive amino acid when taking into account the cellular abundances of amino acids. Moreover, we show that the biosynthesis of a large number of amino acids from glucose and particularly from glycerol provides a surplus of energy, which can be used to balance the high energetic cost of amino acid polymerization. Escherichia coli is widely used in metabolic engineering for biosynthesis of major bio-compounds such as amino acids. In this study, the authors determine the metabolic cost associated with the production of amino acids and proteins on different growth media using several methods. They show that both methionine and leucine are very expensive amino acids. Moreover, the biosynthesis of amino acids from glucose and glycerol can be used to balance the high energetic cost of amino acid polymerization.
Keywords
- Amino acid biosynthetic cost, Amino acid production, Escherichia coli metabolism, Protein production cost, Recombinant proteins
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Medicine
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In: Biotechnology journal, Vol. 8, No. 9, 09.09.2013, p. 1105-1114.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Metabolic costs of amino acid and protein production in Escherichia coli
AU - Kaleta, Christoph
AU - Schäuble, Sascha
AU - Rinas, Ursula
AU - Schuster, Stefan
PY - 2013/9/9
Y1 - 2013/9/9
N2 - Escherichia coli is the most popular microorganism for the production of recombinant proteins and is gaining increasing importance for the production of low-molecular weight compounds such as amino acids. The metabolic cost associated with the production of amino acids and (recombinant) proteins from glucose, glycerol and acetate was determined using three different computational techniques to identify those amino acids that put the highest burden on the biosynthetic machinery of E. coli. Comparing the costs of individual amino acids, we find that methionine is the most expensive amino acid in terms of consumed mol of ATP per molecule produced, while leucine is the most expensive amino acid when taking into account the cellular abundances of amino acids. Moreover, we show that the biosynthesis of a large number of amino acids from glucose and particularly from glycerol provides a surplus of energy, which can be used to balance the high energetic cost of amino acid polymerization. Escherichia coli is widely used in metabolic engineering for biosynthesis of major bio-compounds such as amino acids. In this study, the authors determine the metabolic cost associated with the production of amino acids and proteins on different growth media using several methods. They show that both methionine and leucine are very expensive amino acids. Moreover, the biosynthesis of amino acids from glucose and glycerol can be used to balance the high energetic cost of amino acid polymerization.
AB - Escherichia coli is the most popular microorganism for the production of recombinant proteins and is gaining increasing importance for the production of low-molecular weight compounds such as amino acids. The metabolic cost associated with the production of amino acids and (recombinant) proteins from glucose, glycerol and acetate was determined using three different computational techniques to identify those amino acids that put the highest burden on the biosynthetic machinery of E. coli. Comparing the costs of individual amino acids, we find that methionine is the most expensive amino acid in terms of consumed mol of ATP per molecule produced, while leucine is the most expensive amino acid when taking into account the cellular abundances of amino acids. Moreover, we show that the biosynthesis of a large number of amino acids from glucose and particularly from glycerol provides a surplus of energy, which can be used to balance the high energetic cost of amino acid polymerization. Escherichia coli is widely used in metabolic engineering for biosynthesis of major bio-compounds such as amino acids. In this study, the authors determine the metabolic cost associated with the production of amino acids and proteins on different growth media using several methods. They show that both methionine and leucine are very expensive amino acids. Moreover, the biosynthesis of amino acids from glucose and glycerol can be used to balance the high energetic cost of amino acid polymerization.
KW - Amino acid biosynthetic cost
KW - Amino acid production
KW - Escherichia coli metabolism
KW - Protein production cost
KW - Recombinant proteins
UR - http://www.scopus.com/inward/record.url?scp=84883810552&partnerID=8YFLogxK
U2 - 10.1002/biot.201200267
DO - 10.1002/biot.201200267
M3 - Article
C2 - 23744758
AN - SCOPUS:84883810552
VL - 8
SP - 1105
EP - 1114
JO - Biotechnology journal
JF - Biotechnology journal
SN - 1860-6768
IS - 9
ER -