Details
| Original language | English |
|---|---|
| Title of host publication | Optical Interactions with Tissue and Cells XXII |
| Publication status | Published - 8 Feb 2011 |
| Externally published | Yes |
| Event | Optical Interactions with Tissue and Cells XXII - San Francisco, CA, United States Duration: 24 Jan 2011 → 26 Jan 2011 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 7897 |
| ISSN (Print) | 1605-7422 |
Abstract
Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.
Keywords
- Cell surgery, Femtosecond laser, Free radicals, Low-density plasma, Photochemistry
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
Optical Interactions with Tissue and Cells XXII. 2011. 789704 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7897).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Mechanisms of femtosecond laser cell surgery in the low-density plasma regime
AU - Kuetemeyer, Kai
AU - Rezgui, R.
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2011/2/8
Y1 - 2011/2/8
N2 - Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.
AB - Although femtosecond laser cell surgery is widely used for fundamental research in cell biology, the mechanisms in the so-called low-density plasma regime are largely unknown. To date, it is still unclear on which time scales free electron and free radical-induced chemical effects take place leading to intracellular ablation. In this paper, we present our experimental study on the influence of laser parameters and staining on the ablation threshold. We found that the ablation effect resulted from the accumulation of single-shot multiphoton-induced photochemical effects finished within a few nanoseconds. In addition, fluorescence staining of subcellular structures significantly decreased the ablation threshold. Based on our findings, we propose that dye molecules are the major source for providing seed electrons for the ionization cascade.
KW - Cell surgery
KW - Femtosecond laser
KW - Free radicals
KW - Low-density plasma
KW - Photochemistry
UR - http://www.scopus.com/inward/record.url?scp=79955143224&partnerID=8YFLogxK
U2 - 10.1117/12.874147
DO - 10.1117/12.874147
M3 - Conference contribution
AN - SCOPUS:79955143224
SN - 9780819484345
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Interactions with Tissue and Cells XXII
T2 - Optical Interactions with Tissue and Cells XXII
Y2 - 24 January 2011 through 26 January 2011
ER -