Details
| Original language | English |
|---|---|
| Pages (from-to) | 95-123 |
| Number of pages | 29 |
| Journal | Advances in Biochemical Engineering/Biotechnology |
| Volume | 112 |
| Publication status | Published - 2009 |
Abstract
Fracture healing is a complicated process involving many growth factors, cells, and physical forces. In cases, where natural healing is not able, efforts have to be undertaken to improve healing. For this purpose, tissue engineering may be an option. In order to stimulate cells to form a bone tissue several factors are needed: cells, scaffold, and growth factors. Stem cells derived from bone marrow or adipose tissues are the most useful in this regard. The differentiation of the cells can be accelerated using mechanical stimulation. The first part of this chapter describes the influence of longitudinal strain application. The second part uses a sophisticated approach with stem cells on a newly developed biomaterial (Sponceram) in a rotating bed bioreactor with the administration of bone morphogenetic protein-2. It is shown that such an approach is able to produce bone tissue constructs. This may lead to production of larger constructs that can be used in clinical applications.
Keywords
- Biomechanical Phenomena, Bioreactors, Bone Marrow Cells/cytology, Bone Morphogenetic Protein 2/metabolism, Bone and Bones/cytology, Cell Differentiation, Equipment Design, Humans, Intercellular Signaling Peptides and Proteins/metabolism, Mechanotransduction, Cellular, Osteogenesis, Stem Cells/cytology, Tissue Culture Techniques/instrumentation, Tissue Engineering/instrumentation, Tissue Scaffolds
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Advances in Biochemical Engineering/Biotechnology, Vol. 112, 2009, p. 95-123.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Mechanical strain using 2D and 3D bioreactors induces osteogenesis
T2 - implications for bone tissue engineering
AU - van Griensven, M
AU - Diederichs, S
AU - Roeker, S
AU - Boehm, S
AU - Peterbauer, A
AU - Wolbank, S
AU - Riechers, D
AU - Stahl, F
AU - Kasper, C
N1 - Funding information: This work was partially carried out under the scope of the European NoE ?EXPERTISSUES? (NMP3-CT-2004-500283). Solvig Diederichs was a student in the early research training program Marie Curie ?Alea Jacta EST? (MEST-CT-2004-8104). The Sponceram ceramics were kindly provided by Zellwerk GmbH (Oberkr?mer, Germany) and the BMP-2 was a gift from Prof. Walter Sebald (University of W?rzburg).
PY - 2009
Y1 - 2009
N2 - Fracture healing is a complicated process involving many growth factors, cells, and physical forces. In cases, where natural healing is not able, efforts have to be undertaken to improve healing. For this purpose, tissue engineering may be an option. In order to stimulate cells to form a bone tissue several factors are needed: cells, scaffold, and growth factors. Stem cells derived from bone marrow or adipose tissues are the most useful in this regard. The differentiation of the cells can be accelerated using mechanical stimulation. The first part of this chapter describes the influence of longitudinal strain application. The second part uses a sophisticated approach with stem cells on a newly developed biomaterial (Sponceram) in a rotating bed bioreactor with the administration of bone morphogenetic protein-2. It is shown that such an approach is able to produce bone tissue constructs. This may lead to production of larger constructs that can be used in clinical applications.
AB - Fracture healing is a complicated process involving many growth factors, cells, and physical forces. In cases, where natural healing is not able, efforts have to be undertaken to improve healing. For this purpose, tissue engineering may be an option. In order to stimulate cells to form a bone tissue several factors are needed: cells, scaffold, and growth factors. Stem cells derived from bone marrow or adipose tissues are the most useful in this regard. The differentiation of the cells can be accelerated using mechanical stimulation. The first part of this chapter describes the influence of longitudinal strain application. The second part uses a sophisticated approach with stem cells on a newly developed biomaterial (Sponceram) in a rotating bed bioreactor with the administration of bone morphogenetic protein-2. It is shown that such an approach is able to produce bone tissue constructs. This may lead to production of larger constructs that can be used in clinical applications.
KW - Biomechanical Phenomena
KW - Bioreactors
KW - Bone Marrow Cells/cytology
KW - Bone Morphogenetic Protein 2/metabolism
KW - Bone and Bones/cytology
KW - Cell Differentiation
KW - Equipment Design
KW - Humans
KW - Intercellular Signaling Peptides and Proteins/metabolism
KW - Mechanotransduction, Cellular
KW - Osteogenesis
KW - Stem Cells/cytology
KW - Tissue Culture Techniques/instrumentation
KW - Tissue Engineering/instrumentation
KW - Tissue Scaffolds
U2 - 10.1007/978-3-540-69357-4_5
DO - 10.1007/978-3-540-69357-4_5
M3 - Article
C2 - 19290499
VL - 112
SP - 95
EP - 123
JO - Advances in Biochemical Engineering/Biotechnology
JF - Advances in Biochemical Engineering/Biotechnology
SN - 0724-6145
ER -