Details
| Original language | English |
|---|---|
| Pages (from-to) | 1328-1344 |
| Number of pages | 17 |
| Journal | The new phytologist |
| Volume | 244 |
| Issue number | 4 |
| Publication status | Published - 16 Oct 2024 |
Abstract
The initial free expansion of the embryo within a seed is at some point inhibited by its contact with the testa, resulting in its formation of folds and borders. Although less obvious, mechanical forces appear to trigger and accelerate seed maturation. However, the mechanistic basis for this effect remains unclear. Manipulation of the mechanical constraints affecting either the in vivo or in vitro growth of oilseed rape embryos was combined with analytical approaches, including magnetic resonance imaging and computer graphic reconstruction, immunolabelling, flow cytometry, transcriptomic, proteomic, lipidomic and metabolomic profiling. Our data implied that, in vivo, the imposition of mechanical restraints impeded the expansion of testa and endosperm, resulting in the embryo's deformation. An acceleration in embryonic development was implied by the cessation of cell proliferation and the stimulation of lipid and protein storage, characteristic of embryo maturation. The underlying molecular signature included elements of cell cycle control, reactive oxygen species metabolism and transcriptional reprogramming, along with allosteric control of glycolytic flux. Constricting the space allowed for the expansion of in vitro grown embryos induced a similar response. The conclusion is that the imposition of mechanical constraints over the growth of the developing oilseed rape embryo provides an important trigger for its maturation.
Keywords
- Brassica napus, MRI, computer graphic reconstruction, embryogenesis, maturation, mechanical stress, morphogenesis, transcription factors
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: The new phytologist, Vol. 244, No. 4, 16.10.2024, p. 1328-1344.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Mechanical forces orchestrate the metabolism of the developing oilseed rape embryo
AU - Rolletschek, Hardy
AU - Muszynska, Aleksandra
AU - Schwender, Jörg
AU - Radchuk, Volodymyr
AU - Heinemann, Björn
AU - Hilo, Alexander
AU - Plutenko, Iaroslav
AU - Keil, Peter
AU - Ortleb, Stefan
AU - Wagner, Steffen
AU - Kalms, Laura
AU - Gündel, André
AU - Shi, Hai
AU - Fuchs, Jörg
AU - Szymanski, Jedrzej Jakub
AU - Braun, Hans-Peter
AU - Borisjuk, Ljudmilla
N1 - © 2024 The Author(s). New Phytologist © 2024 New Phytologist Foundation.
PY - 2024/10/16
Y1 - 2024/10/16
N2 - The initial free expansion of the embryo within a seed is at some point inhibited by its contact with the testa, resulting in its formation of folds and borders. Although less obvious, mechanical forces appear to trigger and accelerate seed maturation. However, the mechanistic basis for this effect remains unclear. Manipulation of the mechanical constraints affecting either the in vivo or in vitro growth of oilseed rape embryos was combined with analytical approaches, including magnetic resonance imaging and computer graphic reconstruction, immunolabelling, flow cytometry, transcriptomic, proteomic, lipidomic and metabolomic profiling. Our data implied that, in vivo, the imposition of mechanical restraints impeded the expansion of testa and endosperm, resulting in the embryo's deformation. An acceleration in embryonic development was implied by the cessation of cell proliferation and the stimulation of lipid and protein storage, characteristic of embryo maturation. The underlying molecular signature included elements of cell cycle control, reactive oxygen species metabolism and transcriptional reprogramming, along with allosteric control of glycolytic flux. Constricting the space allowed for the expansion of in vitro grown embryos induced a similar response. The conclusion is that the imposition of mechanical constraints over the growth of the developing oilseed rape embryo provides an important trigger for its maturation.
AB - The initial free expansion of the embryo within a seed is at some point inhibited by its contact with the testa, resulting in its formation of folds and borders. Although less obvious, mechanical forces appear to trigger and accelerate seed maturation. However, the mechanistic basis for this effect remains unclear. Manipulation of the mechanical constraints affecting either the in vivo or in vitro growth of oilseed rape embryos was combined with analytical approaches, including magnetic resonance imaging and computer graphic reconstruction, immunolabelling, flow cytometry, transcriptomic, proteomic, lipidomic and metabolomic profiling. Our data implied that, in vivo, the imposition of mechanical restraints impeded the expansion of testa and endosperm, resulting in the embryo's deformation. An acceleration in embryonic development was implied by the cessation of cell proliferation and the stimulation of lipid and protein storage, characteristic of embryo maturation. The underlying molecular signature included elements of cell cycle control, reactive oxygen species metabolism and transcriptional reprogramming, along with allosteric control of glycolytic flux. Constricting the space allowed for the expansion of in vitro grown embryos induced a similar response. The conclusion is that the imposition of mechanical constraints over the growth of the developing oilseed rape embryo provides an important trigger for its maturation.
KW - Brassica napus
KW - MRI
KW - computer graphic reconstruction
KW - embryogenesis
KW - maturation
KW - mechanical stress
KW - morphogenesis
KW - transcription factors
UR - http://www.scopus.com/inward/record.url?scp=85199399590&partnerID=8YFLogxK
U2 - 10.1111/nph.19990
DO - 10.1111/nph.19990
M3 - Article
C2 - 39044722
VL - 244
SP - 1328
EP - 1344
JO - The new phytologist
JF - The new phytologist
SN - 0028-646X
IS - 4
ER -