Details
| Original language | German |
|---|---|
| Pages (from-to) | 1582-1586 |
| Journal | J CHEM EDUC |
| Issue number | 89 (12) |
| Publication status | Published - 2012 |
Abstract
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In: J CHEM EDUC, No. 89 (12), 2012, p. 1582-1586.
Research output: Contribution to journal › Article › Transfer › peer review
}
TY - JOUR
T1 - Measurement of Enzyme Kinetics by Use of a Blood Glucometer: Hydrolysis of Sucrose and Lactose
AU - Heinzerling, Peter
AU - Schrader, Frank
AU - Schanze, Sascha
PY - 2012
Y1 - 2012
N2 - An alternative analytical method for measuring the kinetic parameters of the enzymes invertase and lactase is described. Invertase hydrolyzes sucrose to glucose and fructose and lactase hydrolyzes lactose to glucose and galactose. In most enzyme kinetics studies, photometric methods or test strips are used to quantify the derivates of the substrates. The use of a commercial blood glucose meter to determine the hydrolyzed glucose is described. This inexpensive and efficient method can be used when teaching enzymatic kinetics in lower-level biochemistry laboratories.
AB - An alternative analytical method for measuring the kinetic parameters of the enzymes invertase and lactase is described. Invertase hydrolyzes sucrose to glucose and fructose and lactase hydrolyzes lactose to glucose and galactose. In most enzyme kinetics studies, photometric methods or test strips are used to quantify the derivates of the substrates. The use of a commercial blood glucose meter to determine the hydrolyzed glucose is described. This inexpensive and efficient method can be used when teaching enzymatic kinetics in lower-level biochemistry laboratories.
U2 - dx.doi.org/10.1021/ed200735f
DO - dx.doi.org/10.1021/ed200735f
M3 - Artikel
SP - 1582
EP - 1586
JO - J CHEM EDUC
JF - J CHEM EDUC
SN - 0021-9584
IS - 89 (12)
ER -