Details
| Original language | English |
|---|---|
| Article number | 620 |
| Journal | Frontiers in Plant Science |
| Volume | 9 |
| Publication status | Published - 7 May 2018 |
Abstract
Haploid potato populations derived from a single tetraploid donor constitute an efficient strategy to analyze markers segregating from a single donor genotype. Analysis of marker segregation in populations derived from crosses between polysomic tetraploids is complicated by a maximum of eight segregating alleles, multiple dosages of the markers and problems related to linkage analysis of marker segregation in repulsion. Here, we present data on two monoparental haploid populations generated by prickle pollination of two tetraploid cultivars with Solanum phureja and genotyped with the 12.8k SolCAP single nucleotide polymorphism(SNP) array. We showthat in a population of monoparental haploids, the number of biallelic SNP markers segregating in linkage to loci from the tetraploid donor genotype is much larger than in putative crosses of this genotype to a diverse selection of 125 tetraploid cultivars. Although this strategy is more laborious than conventional breeding, the generation of haploid progeny for efficient marker analysis is straightforward if morphological markers and flow cytometry are utilized to select true haploid progeny. The level of introgressed fragments from S. phureja, the haploid inducer, is very low, supporting its suitability for genetic analysis. Mapping with single-dose markers allowed the analysis of quantitative trait loci (QTL) for four phenotypic traits.
Keywords
- Dihaploid, Linkage, Mapping, QTL, SNPs, Solanum phureja, Tuber
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 9, 620, 07.05.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Maximization of Markers Linked in Coupling for Tetraploid Potatoes via Monoparental Haploids
AU - Bartkiewicz, Annette M.
AU - Chilla, Friederike
AU - Terefe-Ayana, Diro
AU - Lübeck, Jens
AU - Strahwald, Josef
AU - Tacke, Eckhard
AU - Hofferbert, Hans Reinhard
AU - Linde, Marcus
AU - Debener, Thomas
N1 - Funding Information: The study was supported by the Federal Ministry of Food and Agriculture via the Fachagentur Nachwachsende Rohstoffe e.V. (FNR).
PY - 2018/5/7
Y1 - 2018/5/7
N2 - Haploid potato populations derived from a single tetraploid donor constitute an efficient strategy to analyze markers segregating from a single donor genotype. Analysis of marker segregation in populations derived from crosses between polysomic tetraploids is complicated by a maximum of eight segregating alleles, multiple dosages of the markers and problems related to linkage analysis of marker segregation in repulsion. Here, we present data on two monoparental haploid populations generated by prickle pollination of two tetraploid cultivars with Solanum phureja and genotyped with the 12.8k SolCAP single nucleotide polymorphism(SNP) array. We showthat in a population of monoparental haploids, the number of biallelic SNP markers segregating in linkage to loci from the tetraploid donor genotype is much larger than in putative crosses of this genotype to a diverse selection of 125 tetraploid cultivars. Although this strategy is more laborious than conventional breeding, the generation of haploid progeny for efficient marker analysis is straightforward if morphological markers and flow cytometry are utilized to select true haploid progeny. The level of introgressed fragments from S. phureja, the haploid inducer, is very low, supporting its suitability for genetic analysis. Mapping with single-dose markers allowed the analysis of quantitative trait loci (QTL) for four phenotypic traits.
AB - Haploid potato populations derived from a single tetraploid donor constitute an efficient strategy to analyze markers segregating from a single donor genotype. Analysis of marker segregation in populations derived from crosses between polysomic tetraploids is complicated by a maximum of eight segregating alleles, multiple dosages of the markers and problems related to linkage analysis of marker segregation in repulsion. Here, we present data on two monoparental haploid populations generated by prickle pollination of two tetraploid cultivars with Solanum phureja and genotyped with the 12.8k SolCAP single nucleotide polymorphism(SNP) array. We showthat in a population of monoparental haploids, the number of biallelic SNP markers segregating in linkage to loci from the tetraploid donor genotype is much larger than in putative crosses of this genotype to a diverse selection of 125 tetraploid cultivars. Although this strategy is more laborious than conventional breeding, the generation of haploid progeny for efficient marker analysis is straightforward if morphological markers and flow cytometry are utilized to select true haploid progeny. The level of introgressed fragments from S. phureja, the haploid inducer, is very low, supporting its suitability for genetic analysis. Mapping with single-dose markers allowed the analysis of quantitative trait loci (QTL) for four phenotypic traits.
KW - Dihaploid
KW - Linkage
KW - Mapping
KW - QTL
KW - SNPs
KW - Solanum phureja
KW - Tuber
UR - http://www.scopus.com/inward/record.url?scp=85046902738&partnerID=8YFLogxK
U2 - 10.3389/fpls.2018.00620
DO - 10.3389/fpls.2018.00620
M3 - Article
AN - SCOPUS:85046902738
VL - 9
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 620
ER -