Details
| Original language | English |
|---|---|
| Title of host publication | XXI International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals - Part I |
| Publisher | International Society for Horticultural Science |
| Pages | 27-34 |
| Number of pages | 8 |
| ISBN (print) | 9789066051478 |
| Publication status | Published - 2003 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 612 |
| ISSN (Print) | 0567-7572 |
Abstract
With the aim of achieving storable somatic embryos of Cyclamen persicum, different desiccation conditions and two rehydration methods have been tested. In addition, elevated sucrose concentration and polyethylene glycol (PEG 1500) have been applied during differentiation in order to find appropriate maturation conditions. Somatic embryos were dried above saturated salt solutions adjusting different relative humidities (RH) in desiccators. After drying a four-hour-prehydrationtreatment in 98 % RH was performed. The method used thereafter for rehydration was most important for subsequent germination. Only after imbibition in liquid hormone-free medium tubers, cotyledons, and roots were formed. Embryos dried slowly to moisture contents of 24 % and higher were able to germinate. However, drying in decreasing RHs (91 - 57 - 18 %) resulted in very low water contents of about 12%, which is comparable to that found in seeds. When applying decreasing RHs, somatic embryos with high dry matter content showed germination rates of up to 60 %. However, depending on the quality of somatic embryos strong differences were observed between experiments. Germinating embryos formed bigger tubers than non-dried controls and developed cotyledons of better quality. Treatment with 60 g/l sucrose during the three weeks of differentiation resulted in an increased number of single somatic embryos with higher dry matter content and improved desiccation tolerance.
Keywords
- Dehydration, In vitro, Ornamentals, Rehydration, Storage
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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XXI International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals - Part I. International Society for Horticultural Science, 2003. p. 27-34 (Acta Horticulturae; Vol. 612).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Maturation and desiccation of somatic embryos of cyclamen persicum
AU - Winkelmann, Traud
AU - Meyer, Lara
AU - Serek, Margrethe
PY - 2003
Y1 - 2003
N2 - With the aim of achieving storable somatic embryos of Cyclamen persicum, different desiccation conditions and two rehydration methods have been tested. In addition, elevated sucrose concentration and polyethylene glycol (PEG 1500) have been applied during differentiation in order to find appropriate maturation conditions. Somatic embryos were dried above saturated salt solutions adjusting different relative humidities (RH) in desiccators. After drying a four-hour-prehydrationtreatment in 98 % RH was performed. The method used thereafter for rehydration was most important for subsequent germination. Only after imbibition in liquid hormone-free medium tubers, cotyledons, and roots were formed. Embryos dried slowly to moisture contents of 24 % and higher were able to germinate. However, drying in decreasing RHs (91 - 57 - 18 %) resulted in very low water contents of about 12%, which is comparable to that found in seeds. When applying decreasing RHs, somatic embryos with high dry matter content showed germination rates of up to 60 %. However, depending on the quality of somatic embryos strong differences were observed between experiments. Germinating embryos formed bigger tubers than non-dried controls and developed cotyledons of better quality. Treatment with 60 g/l sucrose during the three weeks of differentiation resulted in an increased number of single somatic embryos with higher dry matter content and improved desiccation tolerance.
AB - With the aim of achieving storable somatic embryos of Cyclamen persicum, different desiccation conditions and two rehydration methods have been tested. In addition, elevated sucrose concentration and polyethylene glycol (PEG 1500) have been applied during differentiation in order to find appropriate maturation conditions. Somatic embryos were dried above saturated salt solutions adjusting different relative humidities (RH) in desiccators. After drying a four-hour-prehydrationtreatment in 98 % RH was performed. The method used thereafter for rehydration was most important for subsequent germination. Only after imbibition in liquid hormone-free medium tubers, cotyledons, and roots were formed. Embryos dried slowly to moisture contents of 24 % and higher were able to germinate. However, drying in decreasing RHs (91 - 57 - 18 %) resulted in very low water contents of about 12%, which is comparable to that found in seeds. When applying decreasing RHs, somatic embryos with high dry matter content showed germination rates of up to 60 %. However, depending on the quality of somatic embryos strong differences were observed between experiments. Germinating embryos formed bigger tubers than non-dried controls and developed cotyledons of better quality. Treatment with 60 g/l sucrose during the three weeks of differentiation resulted in an increased number of single somatic embryos with higher dry matter content and improved desiccation tolerance.
KW - Dehydration
KW - In vitro
KW - Ornamentals
KW - Rehydration
KW - Storage
UR - http://www.scopus.com/inward/record.url?scp=3142750520&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2003.612.3
DO - 10.17660/ActaHortic.2003.612.3
M3 - Conference contribution
AN - SCOPUS:3142750520
SN - 9789066051478
T3 - Acta Horticulturae
SP - 27
EP - 34
BT - XXI International Eucarpia Symposium on Classical versus Molecular Breeding of Ornamentals - Part I
PB - International Society for Horticultural Science
ER -