Details
| Original language | English |
|---|---|
| Pages (from-to) | 616-626 |
| Number of pages | 11 |
| Journal | Electrochimica acta |
| Volume | 137 |
| Publication status | Published - 10 Aug 2014 |
| Externally published | Yes |
Abstract
1-D model of a porous enzymatic electrode with direct electron transfer mechanism has been developed. As a model reaction, hydrogen peroxide reduction catalyzed by Horseradish Peroxidase has been chosen. The model description includes material and charge balances in different phases as well as detailed kinetics of bioelectrochemical hydrogen peroxide reduction. The model has been solved numerically and validated experimentally under steady state conditions. To investigate the influence of the electrode structure and the immobilization procedure, two types of enzymatic electrodes have been developed. In one procedure (Vulcan-PVDF) enzymes were entrapped into a porous conductive matrix, while in the second one (Vulcan-Gelatin) gelatin was used as a binder and enzymes were cross-linked. The performances of Vulcan-PVDF electrodes were significantly better than of Vulcan-Gelatin electrodes under all studied conditions. According to the model, the main reasons for this observation are higher number of active enzymes and higher diffusivity of hydrogen peroxide in the catalyst layer (CL) in case of Vulcan-PVDF procedure. The model pointed out that the major limitation in both studied systems is mass transfer limitation. Enzyme utilization in both systems is very low.
Keywords
- direct electron transfer, Horseradish Peroxidase, modeling, Porous enzymatic electrode, Vulcan nanoparticles
ASJC Scopus subject areas
- Chemical Engineering(all)
- General Chemical Engineering
- Chemistry(all)
- Electrochemistry
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Electrochimica acta, Vol. 137, 10.08.2014, p. 616-626.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Mathematical Modeling of a Porous Enzymatic Electrode with Direct Electron Transfer Mechanism
AU - Do, T. Q.N.
AU - Varničić, M.
AU - Hanke-Rauschenbach, R.
AU - Vidaković-Koch, T.
AU - Sundmacher, K.
N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/8/10
Y1 - 2014/8/10
N2 - 1-D model of a porous enzymatic electrode with direct electron transfer mechanism has been developed. As a model reaction, hydrogen peroxide reduction catalyzed by Horseradish Peroxidase has been chosen. The model description includes material and charge balances in different phases as well as detailed kinetics of bioelectrochemical hydrogen peroxide reduction. The model has been solved numerically and validated experimentally under steady state conditions. To investigate the influence of the electrode structure and the immobilization procedure, two types of enzymatic electrodes have been developed. In one procedure (Vulcan-PVDF) enzymes were entrapped into a porous conductive matrix, while in the second one (Vulcan-Gelatin) gelatin was used as a binder and enzymes were cross-linked. The performances of Vulcan-PVDF electrodes were significantly better than of Vulcan-Gelatin electrodes under all studied conditions. According to the model, the main reasons for this observation are higher number of active enzymes and higher diffusivity of hydrogen peroxide in the catalyst layer (CL) in case of Vulcan-PVDF procedure. The model pointed out that the major limitation in both studied systems is mass transfer limitation. Enzyme utilization in both systems is very low.
AB - 1-D model of a porous enzymatic electrode with direct electron transfer mechanism has been developed. As a model reaction, hydrogen peroxide reduction catalyzed by Horseradish Peroxidase has been chosen. The model description includes material and charge balances in different phases as well as detailed kinetics of bioelectrochemical hydrogen peroxide reduction. The model has been solved numerically and validated experimentally under steady state conditions. To investigate the influence of the electrode structure and the immobilization procedure, two types of enzymatic electrodes have been developed. In one procedure (Vulcan-PVDF) enzymes were entrapped into a porous conductive matrix, while in the second one (Vulcan-Gelatin) gelatin was used as a binder and enzymes were cross-linked. The performances of Vulcan-PVDF electrodes were significantly better than of Vulcan-Gelatin electrodes under all studied conditions. According to the model, the main reasons for this observation are higher number of active enzymes and higher diffusivity of hydrogen peroxide in the catalyst layer (CL) in case of Vulcan-PVDF procedure. The model pointed out that the major limitation in both studied systems is mass transfer limitation. Enzyme utilization in both systems is very low.
KW - direct electron transfer
KW - Horseradish Peroxidase
KW - modeling
KW - Porous enzymatic electrode
KW - Vulcan nanoparticles
KW - direct electron transfer
KW - Horseradish Peroxidase
KW - modeling
KW - Porous enzymatic electrode
KW - Vulcan nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84903704013&partnerID=8YFLogxK
U2 - 10.1016/j.electacta.2014.06.031
DO - 10.1016/j.electacta.2014.06.031
M3 - Article
AN - SCOPUS:84903704013
VL - 137
SP - 616
EP - 626
JO - Electrochimica acta
JF - Electrochimica acta
SN - 0013-4686
ER -