Details
| Original language | English |
|---|---|
| Pages (from-to) | 415-418 |
| Number of pages | 4 |
| Journal | Biology and fertility of soils |
| Volume | 46 |
| Issue number | 4 |
| Publication status | Published - Apr 2010 |
| Externally published | Yes |
Abstract
The antibiotic sulfadiazine (SDZ) can affect denitrifying bacteria in soil. However, effects on denitrifiers in the gut of earthworms have not been described so far. Therefore, the influence of SDZ-contaminated manure applied to soil on denitrifiers in the gut of the earthworm Eisenia fetida was assessed by quantitative polymerase chain reaction targeting genes coding for nirK- and nirS-type nitrite reductases of denitrifiers. Gut contents of Eisenia fetida contained 2.5 × 106 and 5.1 × 105 gene copies of nirK and nirS, respectively, after 2 weeks in soils amended with manure only. Copy numbers of nirK and nirS in gut contents from manure treatments with SDZ were up to ten times less. Overall, the data indicate a negative impact of SDZ on denitrifiers in the gut of earthworms.
Keywords
- Antibiotic, Denitrifiers, Eisenia fetida, nirK, nirS, Real-time PCR, Sulfadiazine, Worm gut
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Soil Science
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In: Biology and fertility of soils, Vol. 46, No. 4, 04.2010, p. 415-418.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Manure contaminated with the antibiotic sulfadiazine impairs the abundance of nirK- and nirS-type denitrifiers in the gut of the earthworm Eisenia fetida
AU - Kotzerke, Anja
AU - Klemer, Sven
AU - Kleineidam, Kristina
AU - Horn, Marcus A.
AU - Drake, Harold L.
AU - Schloter, Michael
AU - Wilke, Berndt Michael
N1 - Funding Information: Acknowledgments We acknowledge the financial support provided by German Research Foundation DFG as a part of the research group: Veterinary Medicines in Soils—Basic Research for Risk Analysis. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/4
Y1 - 2010/4
N2 - The antibiotic sulfadiazine (SDZ) can affect denitrifying bacteria in soil. However, effects on denitrifiers in the gut of earthworms have not been described so far. Therefore, the influence of SDZ-contaminated manure applied to soil on denitrifiers in the gut of the earthworm Eisenia fetida was assessed by quantitative polymerase chain reaction targeting genes coding for nirK- and nirS-type nitrite reductases of denitrifiers. Gut contents of Eisenia fetida contained 2.5 × 106 and 5.1 × 105 gene copies of nirK and nirS, respectively, after 2 weeks in soils amended with manure only. Copy numbers of nirK and nirS in gut contents from manure treatments with SDZ were up to ten times less. Overall, the data indicate a negative impact of SDZ on denitrifiers in the gut of earthworms.
AB - The antibiotic sulfadiazine (SDZ) can affect denitrifying bacteria in soil. However, effects on denitrifiers in the gut of earthworms have not been described so far. Therefore, the influence of SDZ-contaminated manure applied to soil on denitrifiers in the gut of the earthworm Eisenia fetida was assessed by quantitative polymerase chain reaction targeting genes coding for nirK- and nirS-type nitrite reductases of denitrifiers. Gut contents of Eisenia fetida contained 2.5 × 106 and 5.1 × 105 gene copies of nirK and nirS, respectively, after 2 weeks in soils amended with manure only. Copy numbers of nirK and nirS in gut contents from manure treatments with SDZ were up to ten times less. Overall, the data indicate a negative impact of SDZ on denitrifiers in the gut of earthworms.
KW - Antibiotic
KW - Denitrifiers
KW - Eisenia fetida
KW - nirK
KW - nirS
KW - Real-time PCR
KW - Sulfadiazine
KW - Worm gut
UR - http://www.scopus.com/inward/record.url?scp=77952674671&partnerID=8YFLogxK
U2 - 10.1007/s00374-009-0434-3
DO - 10.1007/s00374-009-0434-3
M3 - Article
AN - SCOPUS:77952674671
VL - 46
SP - 415
EP - 418
JO - Biology and fertility of soils
JF - Biology and fertility of soils
SN - 0178-2762
IS - 4
ER -