Details
| Original language | English |
|---|---|
| Article number | 587012 |
| Number of pages | 15 |
| Journal | Drug Delivery and Translational Research |
| Early online date | 13 Jan 2025 |
| Publication status | E-pub ahead of print - 13 Jan 2025 |
Abstract
Two features of macrophages make them attractive for targeted transport of drugs: they efficiently take up a broad spectrum of nanoparticles (NPs) and, by sensing cytokine gradients, they are attracted to the sites of infection and inflammation. To expand the potential of macrophages as drug carriers, we investigated whether macrophages could be simultaneously coloaded with different types of nanoparticles, thus equipping individual cells with different functionalities. We used superparamagnetic iron oxide NPs (SPIONs), which produce apoptosis-inducing hyperthermia when exposed to an alternating magnetic field (AMF), and co-loaded them on macrophages together with drug-containing NPs (inorganic–organic nanoparticles (IOH-NPs) or mesoporous silica NPs (MSNs)). We show that individual macrophages can take up both SPIONs and drug-loaded NPs efficiently, thereby generating drug-loaded cells susceptible to AMF-induced cell death. Macrophages co-loaded with SPIONs and drug-containing IOH-NPs spontaneously released the drugs at similar rates irrespective of the application of an AMF. Notably, while the spontaneous drug release from macrophages co-loaded with SPIONs and drug-associated MSNs was low, AMF exposure accelerated the drug release. Thus, AMF exposure of SPION/drug-MSN coloaded macrophages provides a simple strategy for trigger-controlled drug release since it does not require any chemical modification of NPs or drugs. Thus, we assume that the coloading of different types of NPs will expand the potential of macrophages for drug delivery.
Keywords
- 4-hydroxytamoxifen, Alternating magnetic field, Inorganic–organic nanoparticles, Macrophages, Mesoporous silica nanoparticles, Superparamagnetic iron oxide nanoparticles
ASJC Scopus subject areas
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmaceutical Science
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In: Drug Delivery and Translational Research, 13.01.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Macrophages co-loaded with drug-associated and superparamagnetic nanoparticles for triggered drug release by alternating magnetic fields
AU - Desai, Omkar
AU - Kumar, Sandhya
AU - Köster, Mario
AU - Ullah, Sami
AU - Sarker, Sushobhan
AU - Hagemann, Valentin
AU - Habib, Mosaieb
AU - Klaassen, Nicole
AU - Notter, Silke
AU - Feldmann, Claus
AU - Ehlert, Nina
AU - Hauser, Hansjörg
AU - Wirth, Dagmar
N1 - Publisher Copyright: © The Author(s) 2025.
PY - 2025/1/13
Y1 - 2025/1/13
N2 - Two features of macrophages make them attractive for targeted transport of drugs: they efficiently take up a broad spectrum of nanoparticles (NPs) and, by sensing cytokine gradients, they are attracted to the sites of infection and inflammation. To expand the potential of macrophages as drug carriers, we investigated whether macrophages could be simultaneously coloaded with different types of nanoparticles, thus equipping individual cells with different functionalities. We used superparamagnetic iron oxide NPs (SPIONs), which produce apoptosis-inducing hyperthermia when exposed to an alternating magnetic field (AMF), and co-loaded them on macrophages together with drug-containing NPs (inorganic–organic nanoparticles (IOH-NPs) or mesoporous silica NPs (MSNs)). We show that individual macrophages can take up both SPIONs and drug-loaded NPs efficiently, thereby generating drug-loaded cells susceptible to AMF-induced cell death. Macrophages co-loaded with SPIONs and drug-containing IOH-NPs spontaneously released the drugs at similar rates irrespective of the application of an AMF. Notably, while the spontaneous drug release from macrophages co-loaded with SPIONs and drug-associated MSNs was low, AMF exposure accelerated the drug release. Thus, AMF exposure of SPION/drug-MSN coloaded macrophages provides a simple strategy for trigger-controlled drug release since it does not require any chemical modification of NPs or drugs. Thus, we assume that the coloading of different types of NPs will expand the potential of macrophages for drug delivery.
AB - Two features of macrophages make them attractive for targeted transport of drugs: they efficiently take up a broad spectrum of nanoparticles (NPs) and, by sensing cytokine gradients, they are attracted to the sites of infection and inflammation. To expand the potential of macrophages as drug carriers, we investigated whether macrophages could be simultaneously coloaded with different types of nanoparticles, thus equipping individual cells with different functionalities. We used superparamagnetic iron oxide NPs (SPIONs), which produce apoptosis-inducing hyperthermia when exposed to an alternating magnetic field (AMF), and co-loaded them on macrophages together with drug-containing NPs (inorganic–organic nanoparticles (IOH-NPs) or mesoporous silica NPs (MSNs)). We show that individual macrophages can take up both SPIONs and drug-loaded NPs efficiently, thereby generating drug-loaded cells susceptible to AMF-induced cell death. Macrophages co-loaded with SPIONs and drug-containing IOH-NPs spontaneously released the drugs at similar rates irrespective of the application of an AMF. Notably, while the spontaneous drug release from macrophages co-loaded with SPIONs and drug-associated MSNs was low, AMF exposure accelerated the drug release. Thus, AMF exposure of SPION/drug-MSN coloaded macrophages provides a simple strategy for trigger-controlled drug release since it does not require any chemical modification of NPs or drugs. Thus, we assume that the coloading of different types of NPs will expand the potential of macrophages for drug delivery.
KW - 4-hydroxytamoxifen
KW - Alternating magnetic field
KW - Inorganic–organic nanoparticles
KW - Macrophages
KW - Mesoporous silica nanoparticles
KW - Superparamagnetic iron oxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85217251797&partnerID=8YFLogxK
U2 - 10.1007/s13346-024-01774-9
DO - 10.1007/s13346-024-01774-9
M3 - Article
C2 - 39804440
AN - SCOPUS:85217251797
JO - Drug Delivery and Translational Research
JF - Drug Delivery and Translational Research
SN - 2190-393X
M1 - 587012
ER -