Details
| Original language | English |
|---|---|
| Pages (from-to) | 548-551 |
| Number of pages | 4 |
| Journal | Procedia Engineering |
| Volume | 120 |
| Publication status | Published - 11 Sept 2015 |
| Event | 29th European Conference on Solid-State Transducers - Freiburg, Germany Duration: 6 Sept 2015 → 9 Sept 2015 Conference number: 29th |
Abstract
To ensure productivity and product quality, the parameters of biotechnological processes need to be monitored. Along temperature or pH, one important parameter is the cell density in the culture medium. In this work, we present a low-cost sensor system for online cell growth monitoring in bioreactors via permittivity measurements based on coplanar transmission lines. To evaluate the sensor, E. coli cultivations are performed. We found a good correlation between optical density of the culture medium and the effective permittivity at a frequency of 1 kHz when the sensor is submerged into the culture medium. Measurements at higher frequencies additionally allow monitoring the osmolarity. Furthermore, an improved sensor was successfully used for first noninvasive measurements through the polymer wall of a disposable bioreactor.
Keywords
- Cell growth monitoring, Coplanar transmission line, Dielectric spectroscopy, Permittivity measurements, Single use bioreactor
ASJC Scopus subject areas
- Engineering(all)
- General Engineering
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In: Procedia Engineering, Vol. 120, 11.09.2015, p. 548-551.
Research output: Contribution to journal › Conference article › Research › peer review
}
TY - JOUR
T1 - Low-cost Sensor System for Non-invasive Monitoring of Cell Growth in Disposable Bioreactors
AU - Reinecke, T.
AU - Biechele, P.
AU - Schulte, V.
AU - Scheper, T.
AU - Zimmermann, S.
N1 - Conference code: 29th
PY - 2015/9/11
Y1 - 2015/9/11
N2 - To ensure productivity and product quality, the parameters of biotechnological processes need to be monitored. Along temperature or pH, one important parameter is the cell density in the culture medium. In this work, we present a low-cost sensor system for online cell growth monitoring in bioreactors via permittivity measurements based on coplanar transmission lines. To evaluate the sensor, E. coli cultivations are performed. We found a good correlation between optical density of the culture medium and the effective permittivity at a frequency of 1 kHz when the sensor is submerged into the culture medium. Measurements at higher frequencies additionally allow monitoring the osmolarity. Furthermore, an improved sensor was successfully used for first noninvasive measurements through the polymer wall of a disposable bioreactor.
AB - To ensure productivity and product quality, the parameters of biotechnological processes need to be monitored. Along temperature or pH, one important parameter is the cell density in the culture medium. In this work, we present a low-cost sensor system for online cell growth monitoring in bioreactors via permittivity measurements based on coplanar transmission lines. To evaluate the sensor, E. coli cultivations are performed. We found a good correlation between optical density of the culture medium and the effective permittivity at a frequency of 1 kHz when the sensor is submerged into the culture medium. Measurements at higher frequencies additionally allow monitoring the osmolarity. Furthermore, an improved sensor was successfully used for first noninvasive measurements through the polymer wall of a disposable bioreactor.
KW - Cell growth monitoring
KW - Coplanar transmission line
KW - Dielectric spectroscopy
KW - Permittivity measurements
KW - Single use bioreactor
UR - http://www.scopus.com/inward/record.url?scp=84985010348&partnerID=8YFLogxK
U2 - 10.1016/j.proeng.2015.08.712
DO - 10.1016/j.proeng.2015.08.712
M3 - Conference article
AN - SCOPUS:84985010348
VL - 120
SP - 548
EP - 551
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
T2 - 29th European Conference on Solid-State Transducers
Y2 - 6 September 2015 through 9 September 2015
ER -