Details
| Original language | English |
|---|---|
| Pages (from-to) | 51-64 |
| Number of pages | 14 |
| Journal | Stem cell research |
| Volume | 5 |
| Issue number | 1 |
| Publication status | Published - 30 Mar 2010 |
Abstract
Therapeutic application of stem cell derivatives requires large quantities of cells produced in defined media that cannot be produced via conventional adherent culture. We have applied human induced pluripotent stem (hiPS) cells expressing eGFP under control of the OCT4 promoter to establish the expansion of undifferentiated human embryonic stem (hES) and hiPS cells in suspension culture. A defined culture medium has been identified that results in up to six-fold increase in cell numbers within four days. Our culture system is based on initial single cell dissociation which is critical for standardized process inoculation. HES / hiPS cells were expanded for up to 17 passages. The cells maintained a stable karyotype, their expression of pluripotency markers and their potential to differentiate into derivatives of all three germ layers. The ability to expand HES / hiPS cells in a scalable suspension culture represents a critical step towards standardized production in stirred bioreactors.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Developmental Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
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In: Stem cell research, Vol. 5, No. 1, 30.03.2010, p. 51-64.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Long term expansion of undifferentiated human iPS and ES cells in suspension culture using a defined medium
AU - Olmer, Ruth
AU - Haase, Alexandra
AU - Merkert, Sylvia
AU - Cui, Wei
AU - Paleček, Jiří
AU - Ran, Chen
AU - Kirschning, Andreas
AU - Scheper, Thomas
AU - Glage, Silke
AU - Miller, Konstantin
AU - Curnow, Eliza C.
AU - Hayes, Eric S.
AU - Martin, Ulrich
PY - 2010/3/30
Y1 - 2010/3/30
N2 - Therapeutic application of stem cell derivatives requires large quantities of cells produced in defined media that cannot be produced via conventional adherent culture. We have applied human induced pluripotent stem (hiPS) cells expressing eGFP under control of the OCT4 promoter to establish the expansion of undifferentiated human embryonic stem (hES) and hiPS cells in suspension culture. A defined culture medium has been identified that results in up to six-fold increase in cell numbers within four days. Our culture system is based on initial single cell dissociation which is critical for standardized process inoculation. HES / hiPS cells were expanded for up to 17 passages. The cells maintained a stable karyotype, their expression of pluripotency markers and their potential to differentiate into derivatives of all three germ layers. The ability to expand HES / hiPS cells in a scalable suspension culture represents a critical step towards standardized production in stirred bioreactors.
AB - Therapeutic application of stem cell derivatives requires large quantities of cells produced in defined media that cannot be produced via conventional adherent culture. We have applied human induced pluripotent stem (hiPS) cells expressing eGFP under control of the OCT4 promoter to establish the expansion of undifferentiated human embryonic stem (hES) and hiPS cells in suspension culture. A defined culture medium has been identified that results in up to six-fold increase in cell numbers within four days. Our culture system is based on initial single cell dissociation which is critical for standardized process inoculation. HES / hiPS cells were expanded for up to 17 passages. The cells maintained a stable karyotype, their expression of pluripotency markers and their potential to differentiate into derivatives of all three germ layers. The ability to expand HES / hiPS cells in a scalable suspension culture represents a critical step towards standardized production in stirred bioreactors.
UR - http://www.scopus.com/inward/record.url?scp=77953291028&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2010.03.005
DO - 10.1016/j.scr.2010.03.005
M3 - Article
C2 - 20478754
AN - SCOPUS:77953291028
VL - 5
SP - 51
EP - 64
JO - Stem cell research
JF - Stem cell research
SN - 1873-5061
IS - 1
ER -