Details
| Original language | English |
|---|---|
| Pages (from-to) | 210-214 |
| Number of pages | 5 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 41 |
| Issue number | 2 |
| Publication status | Published - 1 Apr 1994 |
Abstract
Pellets of Tyromyces sambuceus were cultured in standard liquid media that contained a natural triacylglycerol as an additional carbon source. Parallel to a drop of pH below 2.0 and the formation of R(+)-4-decanolide, the oil droplets disappeared. Assays based on 3-naphthyl ester showed that most of the lipolytic and esterolytic enzyme activities were membrane-associated. The time course of enzyme activities after addition of castor oil suggested an inducibility of both membrane-bound and intracellular lipases, while only the intracellular esterases responded significantly. The results could contribute to improving the biotechnological generation of lipid-derived flavours and other compounds.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 41, No. 2, 01.04.1994, p. 210-214.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Localization of lipolytic and esterolytic activities of Tyromyces sambuceus, a 4-decanolide-producing basidiomycete
AU - Hädrich-Meyer, S.
AU - Berger, R. G.
PY - 1994/4/1
Y1 - 1994/4/1
N2 - Pellets of Tyromyces sambuceus were cultured in standard liquid media that contained a natural triacylglycerol as an additional carbon source. Parallel to a drop of pH below 2.0 and the formation of R(+)-4-decanolide, the oil droplets disappeared. Assays based on 3-naphthyl ester showed that most of the lipolytic and esterolytic enzyme activities were membrane-associated. The time course of enzyme activities after addition of castor oil suggested an inducibility of both membrane-bound and intracellular lipases, while only the intracellular esterases responded significantly. The results could contribute to improving the biotechnological generation of lipid-derived flavours and other compounds.
AB - Pellets of Tyromyces sambuceus were cultured in standard liquid media that contained a natural triacylglycerol as an additional carbon source. Parallel to a drop of pH below 2.0 and the formation of R(+)-4-decanolide, the oil droplets disappeared. Assays based on 3-naphthyl ester showed that most of the lipolytic and esterolytic enzyme activities were membrane-associated. The time course of enzyme activities after addition of castor oil suggested an inducibility of both membrane-bound and intracellular lipases, while only the intracellular esterases responded significantly. The results could contribute to improving the biotechnological generation of lipid-derived flavours and other compounds.
UR - http://www.scopus.com/inward/record.url?scp=0028337084&partnerID=8YFLogxK
U2 - 10.1007/BF00186961
DO - 10.1007/BF00186961
M3 - Article
AN - SCOPUS:0028337084
VL - 41
SP - 210
EP - 214
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 2
ER -