Details
| Original language | English |
|---|---|
| Pages (from-to) | 1992-2000 |
| Number of pages | 9 |
| Journal | Chemistry - An Asian Journal |
| Volume | 5 |
| Issue number | 9 |
| Publication status | Published - 2 Jul 2010 |
| Externally published | Yes |
Abstract
The nonspecific interaction of proteins with surfaces in contact with biofluids leads to adverse problems and is prevented by a biocompat-ible surface coating. The current benchmark material among such coatings is poly(ethylene glycol) (PEG). Herein, we report on the synthesis of linear polyglycerol derivatives as promising alternatives to PEG. Therefore, gold surfaces as a model system are functionalized with a self-assembled monolayer (SAM) by a two-step anhydride coupling and a direct thiol immobilization of linear poly(methyl glycerol) and polyglycerol. Surface plasmon resonance (SPR) spectroscopy reveals both types of functionalized surfaces to be as resistant as PEG towards the adsorption of the test proteins fibrinogen, pepsin, albumin, and lysozyme. Moreover, linear polyglycerols adsorb even less proteins from human plasma than a PEG-modified surface. Additional cell adhesion experiments on linearpoly(methyl glycerol) and polyglycerol-modified surfaces show comparable cell resistance as for a PEG-modified surface. Also, in the case of long-term stability, high cell resistance is observed for all samples in medium. Additional in vitro cell-toxicity tests add to the argument that linear poly(methyl glycer-ol) and polyglycerol are strong candidates for promising alternatives to PEG, which can easily be modified for biocompatible functionalization of other surfaces.
Keywords
- Adsorption, Cell adhesion, Monolayers, Proteins, Self-assembly
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Organic Chemistry
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In: Chemistry - An Asian Journal, Vol. 5, No. 9, 02.07.2010, p. 1992-2000.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Linear poly(methyl glycerol) and linear polyglycerol as potent protein and cell resistant alternatives to poly(ethylene glycol)
AU - Weinhart, Marie
AU - Grunwald, Ingo
AU - Wyszogrodzka, Monika
AU - Gaetjen, Linda
AU - Hartwig, Andreas
AU - Haag, Rainer
PY - 2010/7/2
Y1 - 2010/7/2
N2 - The nonspecific interaction of proteins with surfaces in contact with biofluids leads to adverse problems and is prevented by a biocompat-ible surface coating. The current benchmark material among such coatings is poly(ethylene glycol) (PEG). Herein, we report on the synthesis of linear polyglycerol derivatives as promising alternatives to PEG. Therefore, gold surfaces as a model system are functionalized with a self-assembled monolayer (SAM) by a two-step anhydride coupling and a direct thiol immobilization of linear poly(methyl glycerol) and polyglycerol. Surface plasmon resonance (SPR) spectroscopy reveals both types of functionalized surfaces to be as resistant as PEG towards the adsorption of the test proteins fibrinogen, pepsin, albumin, and lysozyme. Moreover, linear polyglycerols adsorb even less proteins from human plasma than a PEG-modified surface. Additional cell adhesion experiments on linearpoly(methyl glycerol) and polyglycerol-modified surfaces show comparable cell resistance as for a PEG-modified surface. Also, in the case of long-term stability, high cell resistance is observed for all samples in medium. Additional in vitro cell-toxicity tests add to the argument that linear poly(methyl glycer-ol) and polyglycerol are strong candidates for promising alternatives to PEG, which can easily be modified for biocompatible functionalization of other surfaces.
AB - The nonspecific interaction of proteins with surfaces in contact with biofluids leads to adverse problems and is prevented by a biocompat-ible surface coating. The current benchmark material among such coatings is poly(ethylene glycol) (PEG). Herein, we report on the synthesis of linear polyglycerol derivatives as promising alternatives to PEG. Therefore, gold surfaces as a model system are functionalized with a self-assembled monolayer (SAM) by a two-step anhydride coupling and a direct thiol immobilization of linear poly(methyl glycerol) and polyglycerol. Surface plasmon resonance (SPR) spectroscopy reveals both types of functionalized surfaces to be as resistant as PEG towards the adsorption of the test proteins fibrinogen, pepsin, albumin, and lysozyme. Moreover, linear polyglycerols adsorb even less proteins from human plasma than a PEG-modified surface. Additional cell adhesion experiments on linearpoly(methyl glycerol) and polyglycerol-modified surfaces show comparable cell resistance as for a PEG-modified surface. Also, in the case of long-term stability, high cell resistance is observed for all samples in medium. Additional in vitro cell-toxicity tests add to the argument that linear poly(methyl glycer-ol) and polyglycerol are strong candidates for promising alternatives to PEG, which can easily be modified for biocompatible functionalization of other surfaces.
KW - Adsorption
KW - Cell adhesion
KW - Monolayers
KW - Proteins
KW - Self-assembly
UR - http://www.scopus.com/inward/record.url?scp=77956476696&partnerID=8YFLogxK
U2 - 10.1002/asia.201000127
DO - 10.1002/asia.201000127
M3 - Article
C2 - 20602410
AN - SCOPUS:77956476696
VL - 5
SP - 1992
EP - 2000
JO - Chemistry - An Asian Journal
JF - Chemistry - An Asian Journal
SN - 1861-4728
IS - 9
ER -