Details
| Original language | English |
|---|---|
| Pages (from-to) | 42-53 |
| Number of pages | 12 |
| Journal | International Journal of Bioprinting |
| Volume | 3 |
| Issue number | 1 |
| Publication status | Published - 1 Jan 2017 |
Abstract
For more than a decade, living cells and biomaterials (typically hydrogels) are printed via laser-assisted bio- printing. Often, a thin metal layer is applied as laser-absorbing material called dynamic release layer (DRL). This layer is vaporized by focused laser pulses generating vapor pressure that propels forward a coated biomaterial. Different la-sers with laser wavelengths from 193 to 1064 nanometer have been used. As a metal DRL gold, silver, or titanium lay-ers have been used. The applied laser pulse durations were usually in the nanosecond range from 1 to 30 ns. In addition, some studies with femtosecond lasers have been published. However, there are no studies on the effect of all these la-sers parameters on bioprinting with a metal DRL, and on comparing different wavelengths and pulse durations - except one study comparing 500 femtosecond pulses with 15 ns pulses. In this paper, the effects of laser wavelength (355, 532, and 1064 nm) and laser pulse duration (in the range of 8 to 200 ns) are investigated. Furthermore, the effects of laser pulse energy, intensity, and focal spot size are studied. The printed droplet volume, hydrogel jet velocity, and cell via-bility are analyzed.
Keywords
- Bioprinting, Laser absorption layer, Laser parametric study, Laser-assisted bioprinting, Laser-induced forward transfer
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Materials Science(all)
- Materials Science (miscellaneous)
- Engineering(all)
- Industrial and Manufacturing Engineering
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In: International Journal of Bioprinting, Vol. 3, No. 1, 01.01.2017, p. 42-53.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer
T2 - A parametric study
AU - Koch, Lothar
AU - Brandt, Ole
AU - Deiwick, Andrea
AU - Chichkov, Boris
PY - 2017/1/1
Y1 - 2017/1/1
N2 - For more than a decade, living cells and biomaterials (typically hydrogels) are printed via laser-assisted bio- printing. Often, a thin metal layer is applied as laser-absorbing material called dynamic release layer (DRL). This layer is vaporized by focused laser pulses generating vapor pressure that propels forward a coated biomaterial. Different la-sers with laser wavelengths from 193 to 1064 nanometer have been used. As a metal DRL gold, silver, or titanium lay-ers have been used. The applied laser pulse durations were usually in the nanosecond range from 1 to 30 ns. In addition, some studies with femtosecond lasers have been published. However, there are no studies on the effect of all these la-sers parameters on bioprinting with a metal DRL, and on comparing different wavelengths and pulse durations - except one study comparing 500 femtosecond pulses with 15 ns pulses. In this paper, the effects of laser wavelength (355, 532, and 1064 nm) and laser pulse duration (in the range of 8 to 200 ns) are investigated. Furthermore, the effects of laser pulse energy, intensity, and focal spot size are studied. The printed droplet volume, hydrogel jet velocity, and cell via-bility are analyzed.
AB - For more than a decade, living cells and biomaterials (typically hydrogels) are printed via laser-assisted bio- printing. Often, a thin metal layer is applied as laser-absorbing material called dynamic release layer (DRL). This layer is vaporized by focused laser pulses generating vapor pressure that propels forward a coated biomaterial. Different la-sers with laser wavelengths from 193 to 1064 nanometer have been used. As a metal DRL gold, silver, or titanium lay-ers have been used. The applied laser pulse durations were usually in the nanosecond range from 1 to 30 ns. In addition, some studies with femtosecond lasers have been published. However, there are no studies on the effect of all these la-sers parameters on bioprinting with a metal DRL, and on comparing different wavelengths and pulse durations - except one study comparing 500 femtosecond pulses with 15 ns pulses. In this paper, the effects of laser wavelength (355, 532, and 1064 nm) and laser pulse duration (in the range of 8 to 200 ns) are investigated. Furthermore, the effects of laser pulse energy, intensity, and focal spot size are studied. The printed droplet volume, hydrogel jet velocity, and cell via-bility are analyzed.
KW - Bioprinting
KW - Laser absorption layer
KW - Laser parametric study
KW - Laser-assisted bioprinting
KW - Laser-induced forward transfer
UR - http://www.scopus.com/inward/record.url?scp=85027180740&partnerID=8YFLogxK
U2 - 10.18063/IJB.2017.01.001
DO - 10.18063/IJB.2017.01.001
M3 - Article
AN - SCOPUS:85027180740
VL - 3
SP - 42
EP - 53
JO - International Journal of Bioprinting
JF - International Journal of Bioprinting
IS - 1
ER -