Details
| Original language | English |
|---|---|
| Title of host publication | Frontiers in Ultrafast Optics |
| Subtitle of host publication | Biomedical, Scientific, and Industrial Applications XV |
| Editors | Michel Meunier, Peter R. Herman, Stefan Nolte, Alexander Heisterkamp |
| Publisher | SPIE |
| ISBN (electronic) | 9781628414455 |
| Publication status | Published - 9 Mar 2015 |
| Externally published | Yes |
| Event | Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV - San Francisco, United States Duration: 8 Feb 2015 → 10 Feb 2015 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Volume | 9355 |
| ISSN (Print) | 0277-786X |
| ISSN (electronic) | 1996-756X |
Abstract
Laser-based transfection techniques have gained significant interest during the last decade. Either single cell manipulation by focusing on the cell membrane or high-throughput can be realized with laser transfection. The latter is for example provided by gold nanoparticle mediated laser transfection. It is based on the heating of gold nanoparticles through laser irradiation, which permeabilizes the membrane. This technique satisfies most prerequisites of a reliable transfection technique, like efficiency and minimal cell impact. In order to bring it closer to routine usage, we investigated new particle configurations for gold nanoparticle mediated laser transfection. Our setup employs a 532 nm and 850 ps laser system. We immobilized gold particles on cell culture surfaces or modified silica particles with a gold particle surface coverage. Furthermore, first experiments achieving cell perforation with an organic nanoparticle based on polypyrrole were conducted. These three options achieved comparable efficiencies to the incubation of cells with free gold nanoparticles. With regard to the underlying mechanisms of perforation, we performed fluorescence microscopy based imaging of the cell state combined with holographic imaging directly after perforation. First results indicated a power dependent ion (calcium) and volume exchange with the extracellular medium in the first two minutes after perforation. In conclusion, our results can pave the way to a safer and more efficient way of high-throughput laser transfection with gold nanoparticles.
Keywords
- digital holography, gold nanoparticle, high throughput, laser transfection, minimal invasive, nanotechnology
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
Cite this
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- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV. ed. / Michel Meunier; Peter R. Herman; Stefan Nolte; Alexander Heisterkamp. SPIE, 2015. 93550E (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 9355).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Laser transfection with gold nanoparticles
T2 - Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XV
AU - Kalies, S.
AU - Gentemann, L.
AU - Antonopoulos, G. C.
AU - Rakoski, M. S.
AU - Heinemann, D.
AU - Schomaker, M.
AU - Ripken, T.
AU - Meyer, H.
PY - 2015/3/9
Y1 - 2015/3/9
N2 - Laser-based transfection techniques have gained significant interest during the last decade. Either single cell manipulation by focusing on the cell membrane or high-throughput can be realized with laser transfection. The latter is for example provided by gold nanoparticle mediated laser transfection. It is based on the heating of gold nanoparticles through laser irradiation, which permeabilizes the membrane. This technique satisfies most prerequisites of a reliable transfection technique, like efficiency and minimal cell impact. In order to bring it closer to routine usage, we investigated new particle configurations for gold nanoparticle mediated laser transfection. Our setup employs a 532 nm and 850 ps laser system. We immobilized gold particles on cell culture surfaces or modified silica particles with a gold particle surface coverage. Furthermore, first experiments achieving cell perforation with an organic nanoparticle based on polypyrrole were conducted. These three options achieved comparable efficiencies to the incubation of cells with free gold nanoparticles. With regard to the underlying mechanisms of perforation, we performed fluorescence microscopy based imaging of the cell state combined with holographic imaging directly after perforation. First results indicated a power dependent ion (calcium) and volume exchange with the extracellular medium in the first two minutes after perforation. In conclusion, our results can pave the way to a safer and more efficient way of high-throughput laser transfection with gold nanoparticles.
AB - Laser-based transfection techniques have gained significant interest during the last decade. Either single cell manipulation by focusing on the cell membrane or high-throughput can be realized with laser transfection. The latter is for example provided by gold nanoparticle mediated laser transfection. It is based on the heating of gold nanoparticles through laser irradiation, which permeabilizes the membrane. This technique satisfies most prerequisites of a reliable transfection technique, like efficiency and minimal cell impact. In order to bring it closer to routine usage, we investigated new particle configurations for gold nanoparticle mediated laser transfection. Our setup employs a 532 nm and 850 ps laser system. We immobilized gold particles on cell culture surfaces or modified silica particles with a gold particle surface coverage. Furthermore, first experiments achieving cell perforation with an organic nanoparticle based on polypyrrole were conducted. These three options achieved comparable efficiencies to the incubation of cells with free gold nanoparticles. With regard to the underlying mechanisms of perforation, we performed fluorescence microscopy based imaging of the cell state combined with holographic imaging directly after perforation. First results indicated a power dependent ion (calcium) and volume exchange with the extracellular medium in the first two minutes after perforation. In conclusion, our results can pave the way to a safer and more efficient way of high-throughput laser transfection with gold nanoparticles.
KW - digital holography
KW - gold nanoparticle
KW - high throughput
KW - laser transfection
KW - minimal invasive
KW - nanotechnology
UR - http://www.scopus.com/inward/record.url?scp=84930060625&partnerID=8YFLogxK
U2 - 10.1117/12.2077601
DO - 10.1117/12.2077601
M3 - Conference contribution
AN - SCOPUS:84930060625
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Frontiers in Ultrafast Optics
A2 - Meunier, Michel
A2 - Herman, Peter R.
A2 - Nolte, Stefan
A2 - Heisterkamp, Alexander
PB - SPIE
Y2 - 8 February 2015 through 10 February 2015
ER -