Details
| Original language | English |
|---|---|
| Pages (from-to) | 973-982 |
| Number of pages | 10 |
| Journal | Tissue Engineering - Part C: Methods |
| Volume | 17 |
| Issue number | 10 |
| Publication status | Published - 27 Jun 2011 |
| Externally published | Yes |
Abstract
Utilization of living cells for therapies in regenerative medicine requires a fundamental understanding of the interactions between different cells and their environment. Moreover, common models based on adherent two-dimensional cultures are not appropriate to simulate the complex interactions that occur in a three-dimensional (3D) cell-microenvironment in vivo. In this study, we present a computer-aided method for the printing of multiple cell types in a 3D array using laser-assisted bioprinting. By printing spots of human adipose-derived stem cells (ASCs) and endothelial colony-forming cells (ECFCs), we demonstrate that (i) these cell spots can be arranged layer-by-layer in a 3D array; (ii) any cell-cell ratio, cell quantity, cell-type combination, and spot spacing can be realized within this array; and (iii) the height of the 3D array is freely scalable. As a proof of concept, we printed separate spots of ASCs and ECFCs within a 3D array and observed cell-cell interactions in vascular endothelial growth factor-free medium. It has been demonstrated that direct cell-cell contacts trigger the development of stable vascular-like networks. This method can be applied to study complex and dynamic relationships between cells and their local environment.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Engineering(all)
- Biomedical Engineering
Sustainable Development Goals
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In: Tissue Engineering - Part C: Methods, Vol. 17, No. 10, 27.06.2011, p. 973-982.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Laser Printing of Three-Dimensional Multicellular Arrays for Studies of Cell–Celland Cell–Environment Interactions
AU - Gruene, Martin
AU - Pflaum, Michael
AU - Hess, Christian
AU - Diamantouros, Stefanos
AU - Schlie, Sabrina
AU - Deiwick, Andrea
AU - Koch, Lothar
AU - Wilhelmi, Mathias
AU - Jockenhoevel, Stefan
AU - Haverich, Axel
AU - Chichkov, Boris
PY - 2011/6/27
Y1 - 2011/6/27
N2 - Utilization of living cells for therapies in regenerative medicine requires a fundamental understanding of the interactions between different cells and their environment. Moreover, common models based on adherent two-dimensional cultures are not appropriate to simulate the complex interactions that occur in a three-dimensional (3D) cell-microenvironment in vivo. In this study, we present a computer-aided method for the printing of multiple cell types in a 3D array using laser-assisted bioprinting. By printing spots of human adipose-derived stem cells (ASCs) and endothelial colony-forming cells (ECFCs), we demonstrate that (i) these cell spots can be arranged layer-by-layer in a 3D array; (ii) any cell-cell ratio, cell quantity, cell-type combination, and spot spacing can be realized within this array; and (iii) the height of the 3D array is freely scalable. As a proof of concept, we printed separate spots of ASCs and ECFCs within a 3D array and observed cell-cell interactions in vascular endothelial growth factor-free medium. It has been demonstrated that direct cell-cell contacts trigger the development of stable vascular-like networks. This method can be applied to study complex and dynamic relationships between cells and their local environment.
AB - Utilization of living cells for therapies in regenerative medicine requires a fundamental understanding of the interactions between different cells and their environment. Moreover, common models based on adherent two-dimensional cultures are not appropriate to simulate the complex interactions that occur in a three-dimensional (3D) cell-microenvironment in vivo. In this study, we present a computer-aided method for the printing of multiple cell types in a 3D array using laser-assisted bioprinting. By printing spots of human adipose-derived stem cells (ASCs) and endothelial colony-forming cells (ECFCs), we demonstrate that (i) these cell spots can be arranged layer-by-layer in a 3D array; (ii) any cell-cell ratio, cell quantity, cell-type combination, and spot spacing can be realized within this array; and (iii) the height of the 3D array is freely scalable. As a proof of concept, we printed separate spots of ASCs and ECFCs within a 3D array and observed cell-cell interactions in vascular endothelial growth factor-free medium. It has been demonstrated that direct cell-cell contacts trigger the development of stable vascular-like networks. This method can be applied to study complex and dynamic relationships between cells and their local environment.
UR - http://www.scopus.com/inward/record.url?scp=80053297640&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2011.0185
DO - 10.1089/ten.tec.2011.0185
M3 - Article
C2 - 21585313
AN - SCOPUS:80053297640
VL - 17
SP - 973
EP - 982
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
SN - 1937-3384
IS - 10
ER -