Details
| Original language | English |
|---|---|
| Pages (from-to) | 79-87 |
| Number of pages | 9 |
| Journal | Tissue Engineering - Part C: Methods |
| Volume | 17 |
| Issue number | 1 |
| Publication status | Published - 25 Aug 2010 |
Abstract
Stem cells are of widespread interest in regenerative medicine due to their capability of self-renewal and differentiation, which is regulated by their three-dimensional microenvironment. In this study, a computer-aided biofabrication technique based on laser-induced forward transfer (LIFT) is used to generate grafts consisting of mesenchymal stem cells (MSCs). We demonstrate that (i) laser printing does not cause any cell damage; (ii) laser-printed MSC grafts can be differentiated toward bone and cartilage; (iii) LIFT allows printing of cell densities high enough for the promotion of chondrogenesis; (iv) with LIFT three-dimensional scaffold-free autologous tissue grafts can be fabricated keeping their predefined structure, and (v) predifferentiated MSCs survived the complete printing procedure and kept their functionality. We believe that our results will find important applications in stem cell biology and tissue engineering.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Engineering(all)
- Biomedical Engineering
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In: Tissue Engineering - Part C: Methods, Vol. 17, No. 1, 25.08.2010, p. 79-87.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Laser Printing of Stem Cells for Biofabrication of Scaffold-Free Autologous Grafts
AU - Gruene, Martin
AU - Deiwick, Andrea
AU - Koch, Lothar
AU - Schlie, Sabrina
AU - Unger, Claudia
AU - Hofmann, Nicola
AU - Bernemann, Inga
AU - Glasmacher, Birgit
AU - Chichkov, Boris
PY - 2010/8/25
Y1 - 2010/8/25
N2 - Stem cells are of widespread interest in regenerative medicine due to their capability of self-renewal and differentiation, which is regulated by their three-dimensional microenvironment. In this study, a computer-aided biofabrication technique based on laser-induced forward transfer (LIFT) is used to generate grafts consisting of mesenchymal stem cells (MSCs). We demonstrate that (i) laser printing does not cause any cell damage; (ii) laser-printed MSC grafts can be differentiated toward bone and cartilage; (iii) LIFT allows printing of cell densities high enough for the promotion of chondrogenesis; (iv) with LIFT three-dimensional scaffold-free autologous tissue grafts can be fabricated keeping their predefined structure, and (v) predifferentiated MSCs survived the complete printing procedure and kept their functionality. We believe that our results will find important applications in stem cell biology and tissue engineering.
AB - Stem cells are of widespread interest in regenerative medicine due to their capability of self-renewal and differentiation, which is regulated by their three-dimensional microenvironment. In this study, a computer-aided biofabrication technique based on laser-induced forward transfer (LIFT) is used to generate grafts consisting of mesenchymal stem cells (MSCs). We demonstrate that (i) laser printing does not cause any cell damage; (ii) laser-printed MSC grafts can be differentiated toward bone and cartilage; (iii) LIFT allows printing of cell densities high enough for the promotion of chondrogenesis; (iv) with LIFT three-dimensional scaffold-free autologous tissue grafts can be fabricated keeping their predefined structure, and (v) predifferentiated MSCs survived the complete printing procedure and kept their functionality. We believe that our results will find important applications in stem cell biology and tissue engineering.
UR - http://www.scopus.com/inward/record.url?scp=78650862905&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2010.0359
DO - 10.1089/ten.tec.2010.0359
M3 - Article
C2 - 20673023
AN - SCOPUS:78650862905
VL - 17
SP - 79
EP - 87
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
SN - 1937-3384
IS - 1
ER -