Details
| Original language | English |
|---|---|
| Pages (from-to) | 847-854 |
| Number of pages | 8 |
| Journal | Tissue Engineering - Part C: Methods |
| Volume | 16 |
| Issue number | 5 |
| Publication status | Published - 15 Dec 2009 |
| Externally published | Yes |
Abstract
Laser printing based on laser-induced forward transfer (LIFT) is a new biofabrication technique for the arrangement of biological materials or living cells in well-defined patterns. In the current study, skin cell lines (fibroblasts/keratinocytes) and human mesenchymal stem cells (hMSC) were chosen for laser printing experiments due to their high potential in regeneration of human skin and new application possibilities of stem cell therapy. To evaluate the influence of LIFT on the cells, their survival rate, their proliferation and apoptotic activity, and the DNA damages and modifications of their cell surface markers were assessed and statistically evaluated over several days. The cells survived the transfer procedure with a rate of 98%±1% standard error of the mean (skin cells) and 90%±10% (hMSC), respectively. All used cell types maintain their ability to proliferate after LIFT. Further, skin cells and hMSC did not show an increase of apoptosis or DNA fragmentation. In addition, the hMSC keep their phenotype as proven by fluorescence activated cell sorting (FACS) analysis. This study demonstrates LIFT as a suitable technique for unharmed computer-controlled positioning of different cell types and a promising tool for future applications in the ex vivo generation of tissue replacements.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Medicine(all)
- Medicine (miscellaneous)
- Engineering(all)
- Biomedical Engineering
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In: Tissue Engineering - Part C: Methods, Vol. 16, No. 5, 15.12.2009, p. 847-854.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Laser Printing of Skin Cells and Human Stem Cells
AU - Koch, Lothar
AU - Kuhn, Stefanie
AU - Sorg, Heiko
AU - Gruene, Martin
AU - Schlie, Sabrina
AU - Gaebel, Ralf
AU - Polchow, Bianca
AU - Reimers, Kerstin
AU - Stoelting, Stephanie
AU - Ma, Nan
AU - Vogt, Peter M.
AU - Steinhoff, Gustav
AU - Chichkov, Boris
PY - 2009/12/15
Y1 - 2009/12/15
N2 - Laser printing based on laser-induced forward transfer (LIFT) is a new biofabrication technique for the arrangement of biological materials or living cells in well-defined patterns. In the current study, skin cell lines (fibroblasts/keratinocytes) and human mesenchymal stem cells (hMSC) were chosen for laser printing experiments due to their high potential in regeneration of human skin and new application possibilities of stem cell therapy. To evaluate the influence of LIFT on the cells, their survival rate, their proliferation and apoptotic activity, and the DNA damages and modifications of their cell surface markers were assessed and statistically evaluated over several days. The cells survived the transfer procedure with a rate of 98%±1% standard error of the mean (skin cells) and 90%±10% (hMSC), respectively. All used cell types maintain their ability to proliferate after LIFT. Further, skin cells and hMSC did not show an increase of apoptosis or DNA fragmentation. In addition, the hMSC keep their phenotype as proven by fluorescence activated cell sorting (FACS) analysis. This study demonstrates LIFT as a suitable technique for unharmed computer-controlled positioning of different cell types and a promising tool for future applications in the ex vivo generation of tissue replacements.
AB - Laser printing based on laser-induced forward transfer (LIFT) is a new biofabrication technique for the arrangement of biological materials or living cells in well-defined patterns. In the current study, skin cell lines (fibroblasts/keratinocytes) and human mesenchymal stem cells (hMSC) were chosen for laser printing experiments due to their high potential in regeneration of human skin and new application possibilities of stem cell therapy. To evaluate the influence of LIFT on the cells, their survival rate, their proliferation and apoptotic activity, and the DNA damages and modifications of their cell surface markers were assessed and statistically evaluated over several days. The cells survived the transfer procedure with a rate of 98%±1% standard error of the mean (skin cells) and 90%±10% (hMSC), respectively. All used cell types maintain their ability to proliferate after LIFT. Further, skin cells and hMSC did not show an increase of apoptosis or DNA fragmentation. In addition, the hMSC keep their phenotype as proven by fluorescence activated cell sorting (FACS) analysis. This study demonstrates LIFT as a suitable technique for unharmed computer-controlled positioning of different cell types and a promising tool for future applications in the ex vivo generation of tissue replacements.
UR - http://www.scopus.com/inward/record.url?scp=77957358891&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2009.0397
DO - 10.1089/ten.tec.2009.0397
M3 - Article
C2 - 19883209
AN - SCOPUS:77957358891
VL - 16
SP - 847
EP - 854
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
SN - 1937-3384
IS - 5
ER -