Details
| Original language | English |
|---|---|
| Pages (from-to) | 544-549 |
| Number of pages | 6 |
| Journal | Letters in Applied Microbiology |
| Volume | 67 |
| Issue number | 6 |
| Publication status | Published - 1 Dec 2018 |
Abstract
Recently, it was shown that laser-induced forward transfer (LIFT) technology and the laser engineering of microbial systems (LEMS) technique (based on LIFT method) are effective for isolation of micro-organisms from different complex substrates. These techniques frequently utilize Au as an absorbing layer material. The purpose of this study was to investigate the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness of laser printing of micro-organisms to improve LEMS and LIFT techniques. It was shown that application of Ti and Cr absorbing layers activates bacterial growth after laser printing and is significantly more effective in comparison to Au films, which actually show a suppressing effect on bacterial cells. Results of this study can be applied for LEMS and LIFT protocols for improving bacterial isolation and microbial growth. Significance and Impact of the Study: Laser-induced forward transfer technique (LIFT) is currently used for printing of micro-organisms and in biosensor techniques, for single-cell isolation, and for culturing of micro-organisms from complex substrates. We have studied the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness laser printing of micro-organisms. It was shown that application of Ti and Cr absorbing layers activates bacterial growth and is more effective in LIFT compared to Au films, which actually have a suppressive effect on bacteria cells. The results can improve LIFT protocols for bacteria isolation and culturing of microbial systems.
Keywords
- bacteria culturing, bacteria isolation, biodiversity, laser printing, LEMS, LIFT, soil, Bacteria/growth & development, Printing, Titanium/chemistry, Chromium/chemistry, Gold/chemistry, Lasers, Biosensing Techniques, Printing, Three-Dimensional
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Letters in Applied Microbiology, Vol. 67, No. 6, 01.12.2018, p. 544-549.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Laser printing of microbial systems
T2 - effect of absorbing metal film
AU - Cheptsov, V. S.
AU - Churbanova, E. S.
AU - Yusupov, V. I.
AU - Gorlenko, M. V.
AU - Lysak, L. V.
AU - Minaev, N. V.
AU - Bagratashvili, V. N.
AU - Chichkov, B. N.
N1 - © 2018 The Society for Applied Microbiology
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Recently, it was shown that laser-induced forward transfer (LIFT) technology and the laser engineering of microbial systems (LEMS) technique (based on LIFT method) are effective for isolation of micro-organisms from different complex substrates. These techniques frequently utilize Au as an absorbing layer material. The purpose of this study was to investigate the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness of laser printing of micro-organisms to improve LEMS and LIFT techniques. It was shown that application of Ti and Cr absorbing layers activates bacterial growth after laser printing and is significantly more effective in comparison to Au films, which actually show a suppressing effect on bacterial cells. Results of this study can be applied for LEMS and LIFT protocols for improving bacterial isolation and microbial growth. Significance and Impact of the Study: Laser-induced forward transfer technique (LIFT) is currently used for printing of micro-organisms and in biosensor techniques, for single-cell isolation, and for culturing of micro-organisms from complex substrates. We have studied the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness laser printing of micro-organisms. It was shown that application of Ti and Cr absorbing layers activates bacterial growth and is more effective in LIFT compared to Au films, which actually have a suppressive effect on bacteria cells. The results can improve LIFT protocols for bacteria isolation and culturing of microbial systems.
AB - Recently, it was shown that laser-induced forward transfer (LIFT) technology and the laser engineering of microbial systems (LEMS) technique (based on LIFT method) are effective for isolation of micro-organisms from different complex substrates. These techniques frequently utilize Au as an absorbing layer material. The purpose of this study was to investigate the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness of laser printing of micro-organisms to improve LEMS and LIFT techniques. It was shown that application of Ti and Cr absorbing layers activates bacterial growth after laser printing and is significantly more effective in comparison to Au films, which actually show a suppressing effect on bacterial cells. Results of this study can be applied for LEMS and LIFT protocols for improving bacterial isolation and microbial growth. Significance and Impact of the Study: Laser-induced forward transfer technique (LIFT) is currently used for printing of micro-organisms and in biosensor techniques, for single-cell isolation, and for culturing of micro-organisms from complex substrates. We have studied the influence of absorbing film materials (Au, Ti and Cr) on the effectiveness laser printing of micro-organisms. It was shown that application of Ti and Cr absorbing layers activates bacterial growth and is more effective in LIFT compared to Au films, which actually have a suppressive effect on bacteria cells. The results can improve LIFT protocols for bacteria isolation and culturing of microbial systems.
KW - bacteria culturing
KW - bacteria isolation
KW - biodiversity
KW - laser printing
KW - LEMS
KW - LIFT
KW - soil
KW - Bacteria/growth & development
KW - Printing
KW - Titanium/chemistry
KW - Chromium/chemistry
KW - Gold/chemistry
KW - Lasers
KW - Biosensing Techniques
KW - Printing, Three-Dimensional
UR - http://www.scopus.com/inward/record.url?scp=85055258258&partnerID=8YFLogxK
U2 - 10.1111/lam.13074
DO - 10.1111/lam.13074
M3 - Article
C2 - 30223313
AN - SCOPUS:85055258258
VL - 67
SP - 544
EP - 549
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
SN - 0266-8254
IS - 6
ER -