Details
| Original language | English |
|---|---|
| Article number | 035005 |
| Journal | BIOFABRICATION |
| Volume | 10 |
| Issue number | 3 |
| Publication status | Published - 25 Apr 2018 |
Abstract
Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable the generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. Printing of living cells was demonstrated with immortalized cell lines, primary cells, and adult stem cells with different printing technologies and biomaterials. However, hiPSCs are more sensitive to handling procedures, in particular, when dissociated into single cells. Both pluripotency and directed differentiation are influenced by numerous environmental factors including culture media, biomaterials, and cell density. Notably, existing literature on the effect of applied biomaterials on pluripotency is rather ambiguous. In this study, laser bioprinting of undifferentiated hiPSCs in combination with different biomaterials was performed and the impact on cells' behavior, pluripotency, and differentiation was investigated. Our findings suggest that hiPSCs are indeed more sensitive to the applied biomaterials, but not to laser printing itself. With appropriate biomaterials, such as the hyaluronic acid based solutions applied in this study, hiPSCs can be successfully laser printed without losing their pluripotency.
Keywords
- bioprinting, cell pinting, hyaluronic acid, induced pluripotent stem cells, iPSCs, laser-induced forward transfer, Cell Line, Cell Differentiation/drug effects, Bioprinting/methods, Hydrogels, Humans, Ink, Cell Survival/drug effects, Biocompatible Materials/pharmacology, Induced Pluripotent Stem Cells/cytology, Hyaluronic Acid/pharmacology
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Engineering(all)
- Biomedical Engineering
- Materials Science(all)
- Biomaterials
Sustainable Development Goals
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In: BIOFABRICATION, Vol. 10, No. 3, 035005, 25.04.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Laser bioprinting of human induced pluripotent stem cells
T2 - The effect of printing and biomaterials on cell survival, pluripotency, and differentiation
AU - Koch, Lothar
AU - Deiwick, Andrea
AU - Franke, Annika
AU - Schwanke, Kristin
AU - Haverich, Axel
AU - Zweigerdt, Robert
AU - Chichkov, Boris
N1 - © 2018 IOP Publishing Ltd
PY - 2018/4/25
Y1 - 2018/4/25
N2 - Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable the generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. Printing of living cells was demonstrated with immortalized cell lines, primary cells, and adult stem cells with different printing technologies and biomaterials. However, hiPSCs are more sensitive to handling procedures, in particular, when dissociated into single cells. Both pluripotency and directed differentiation are influenced by numerous environmental factors including culture media, biomaterials, and cell density. Notably, existing literature on the effect of applied biomaterials on pluripotency is rather ambiguous. In this study, laser bioprinting of undifferentiated hiPSCs in combination with different biomaterials was performed and the impact on cells' behavior, pluripotency, and differentiation was investigated. Our findings suggest that hiPSCs are indeed more sensitive to the applied biomaterials, but not to laser printing itself. With appropriate biomaterials, such as the hyaluronic acid based solutions applied in this study, hiPSCs can be successfully laser printed without losing their pluripotency.
AB - Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable the generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. Printing of living cells was demonstrated with immortalized cell lines, primary cells, and adult stem cells with different printing technologies and biomaterials. However, hiPSCs are more sensitive to handling procedures, in particular, when dissociated into single cells. Both pluripotency and directed differentiation are influenced by numerous environmental factors including culture media, biomaterials, and cell density. Notably, existing literature on the effect of applied biomaterials on pluripotency is rather ambiguous. In this study, laser bioprinting of undifferentiated hiPSCs in combination with different biomaterials was performed and the impact on cells' behavior, pluripotency, and differentiation was investigated. Our findings suggest that hiPSCs are indeed more sensitive to the applied biomaterials, but not to laser printing itself. With appropriate biomaterials, such as the hyaluronic acid based solutions applied in this study, hiPSCs can be successfully laser printed without losing their pluripotency.
KW - bioprinting
KW - cell pinting
KW - hyaluronic acid
KW - induced pluripotent stem cells
KW - iPSCs
KW - laser-induced forward transfer
KW - Cell Line
KW - Cell Differentiation/drug effects
KW - Bioprinting/methods
KW - Hydrogels
KW - Humans
KW - Ink
KW - Cell Survival/drug effects
KW - Biocompatible Materials/pharmacology
KW - Induced Pluripotent Stem Cells/cytology
KW - Hyaluronic Acid/pharmacology
UR - http://www.scopus.com/inward/record.url?scp=85049927060&partnerID=8YFLogxK
U2 - 10.1088/1758-5090/aab981
DO - 10.1088/1758-5090/aab981
M3 - Article
C2 - 29578448
AN - SCOPUS:85049927060
VL - 10
JO - BIOFABRICATION
JF - BIOFABRICATION
SN - 1758-5082
IS - 3
M1 - 035005
ER -