Details
| Original language | English |
|---|---|
| Pages (from-to) | 136-143 |
| Number of pages | 8 |
| Journal | Sensors and Actuators, A: Physical |
| Volume | 215 |
| Publication status | Published - 3 Jan 2014 |
| Externally published | Yes |
Abstract
A novel lab-on-a-chip is presented for the high-throughput preparation of mammalian cell samples for metabolic studies associated with cell compartmentation. The chip is directly connected to an overpressurized bioreactor, for a continuous harvest and manipulation of cells during the cultivation process. The proposed on-chip preparation technique allows the rapid cell permeabilization and separation of sub-cellular components as cytoplasm and mitochondria, both primarily responsible for cell metabolism. The microfluidic chip comprises two temperature zones (37 °C to ensure physiological conditions and 4 °C for cell quenching), which are thermally decoupled. Cells are manipulated in a continuous flow process in five connected functional modules. The key modules of the integrated chip are a rapid split-and-recombine micromixer for biological system perturbation and chemical lysis, a chaotic advection "stirred" incubation channel for reduced residence time distribution, and a spiral channel for efficient particle separation and media exchange. The bioreactor-chip system was characterized with Chinese hamster ovary cells. It exhibits a high efficiency in the lysis process and separation of sub-cellular components.
Keywords
- Lab-on-a-chip, Mammalian cell preparation, Metabolomics, Organelle separation, Thermal decoupling
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Instrumentation
- Physics and Astronomy(all)
- Condensed Matter Physics
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Engineering(all)
- Electrical and Electronic Engineering
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In: Sensors and Actuators, A: Physical, Vol. 215, 03.01.2014, p. 136-143.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Lab-on-a-chip for cell perturbation, lysis, and efficient separation of sub-cellular components in a continuous flow mode
AU - Rajabi, Negar
AU - Bahnemann, Janina
AU - Tzeng, Tzu Nen
AU - Platas Barradas, Oscar
AU - Zeng, An Ping
AU - Müller, Jörg
N1 - Funding information: The authors would like to thank Grischa Fuge, Uwe Jandt, Sabrina Kayo, and Simon Kern for their support in the bio-lab. The authors also gratefully acknowledge Thomas Noll from the Institute of Cell Culture Technology (University Bielefeld, Germany) for kindly supplying the CHO-K1 cells for cultivation and experiments. This research project (SysCompart, project ID 031555D) is funded by the German Federal Ministry of Research and Education (BMBF) .
PY - 2014/1/3
Y1 - 2014/1/3
N2 - A novel lab-on-a-chip is presented for the high-throughput preparation of mammalian cell samples for metabolic studies associated with cell compartmentation. The chip is directly connected to an overpressurized bioreactor, for a continuous harvest and manipulation of cells during the cultivation process. The proposed on-chip preparation technique allows the rapid cell permeabilization and separation of sub-cellular components as cytoplasm and mitochondria, both primarily responsible for cell metabolism. The microfluidic chip comprises two temperature zones (37 °C to ensure physiological conditions and 4 °C for cell quenching), which are thermally decoupled. Cells are manipulated in a continuous flow process in five connected functional modules. The key modules of the integrated chip are a rapid split-and-recombine micromixer for biological system perturbation and chemical lysis, a chaotic advection "stirred" incubation channel for reduced residence time distribution, and a spiral channel for efficient particle separation and media exchange. The bioreactor-chip system was characterized with Chinese hamster ovary cells. It exhibits a high efficiency in the lysis process and separation of sub-cellular components.
AB - A novel lab-on-a-chip is presented for the high-throughput preparation of mammalian cell samples for metabolic studies associated with cell compartmentation. The chip is directly connected to an overpressurized bioreactor, for a continuous harvest and manipulation of cells during the cultivation process. The proposed on-chip preparation technique allows the rapid cell permeabilization and separation of sub-cellular components as cytoplasm and mitochondria, both primarily responsible for cell metabolism. The microfluidic chip comprises two temperature zones (37 °C to ensure physiological conditions and 4 °C for cell quenching), which are thermally decoupled. Cells are manipulated in a continuous flow process in five connected functional modules. The key modules of the integrated chip are a rapid split-and-recombine micromixer for biological system perturbation and chemical lysis, a chaotic advection "stirred" incubation channel for reduced residence time distribution, and a spiral channel for efficient particle separation and media exchange. The bioreactor-chip system was characterized with Chinese hamster ovary cells. It exhibits a high efficiency in the lysis process and separation of sub-cellular components.
KW - Lab-on-a-chip
KW - Mammalian cell preparation
KW - Metabolomics
KW - Organelle separation
KW - Thermal decoupling
UR - http://www.scopus.com/inward/record.url?scp=84903278233&partnerID=8YFLogxK
U2 - 10.1016/j.sna.2013.12.019
DO - 10.1016/j.sna.2013.12.019
M3 - Article
AN - SCOPUS:84903278233
VL - 215
SP - 136
EP - 143
JO - Sensors and Actuators, A: Physical
JF - Sensors and Actuators, A: Physical
SN - 0924-4247
ER -