Details
| Original language | English |
|---|---|
| Pages (from-to) | 137-147 |
| Number of pages | 11 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 15 |
| Issue number | 2 |
| Publication status | Published - Jan 1988 |
| Externally published | Yes |
Abstract
The time course of the activities of esterase, α-galactosidase, and β-glucosidase in cell sap and nutrient medium in in vitro cultured apple cells (Malus sylvestris Mill.) was studied. The corresponding isozyme patterns and the intracellular and extracellular isozyme patterns of acid phosphatase and polyphenol oxidase were compared using isozyme visualization methods adapted to ultra-thin-layer isoelectric focusing. Neither quantitative (total activity) nor qualitative (isozyme pattern) data were congruent for cell saps and nutrient media. Malate dehydrogenase, malic enzyme, and glutamate dehydrogenase occurred in cell sap only. The extracellular activities probably originate to a great part from a programmed release by intact cells. Nutrient media of plant cell cultures constitute a rich source of active plant isozymes.
Keywords
- α-galactosidase, β-glucosidase, acid phosphatase, apple cell culture, esterase, intra-and extracellular enzymes
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 15, No. 2, 01.1988, p. 137-147.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Isozymes in nutrient medium of suspension-cultured apple cells (Malus sylvestris Mill.)
AU - Berger, Ralf G.
AU - Drawert, Friedrich
AU - Kunz, Christa
PY - 1988/1
Y1 - 1988/1
N2 - The time course of the activities of esterase, α-galactosidase, and β-glucosidase in cell sap and nutrient medium in in vitro cultured apple cells (Malus sylvestris Mill.) was studied. The corresponding isozyme patterns and the intracellular and extracellular isozyme patterns of acid phosphatase and polyphenol oxidase were compared using isozyme visualization methods adapted to ultra-thin-layer isoelectric focusing. Neither quantitative (total activity) nor qualitative (isozyme pattern) data were congruent for cell saps and nutrient media. Malate dehydrogenase, malic enzyme, and glutamate dehydrogenase occurred in cell sap only. The extracellular activities probably originate to a great part from a programmed release by intact cells. Nutrient media of plant cell cultures constitute a rich source of active plant isozymes.
AB - The time course of the activities of esterase, α-galactosidase, and β-glucosidase in cell sap and nutrient medium in in vitro cultured apple cells (Malus sylvestris Mill.) was studied. The corresponding isozyme patterns and the intracellular and extracellular isozyme patterns of acid phosphatase and polyphenol oxidase were compared using isozyme visualization methods adapted to ultra-thin-layer isoelectric focusing. Neither quantitative (total activity) nor qualitative (isozyme pattern) data were congruent for cell saps and nutrient media. Malate dehydrogenase, malic enzyme, and glutamate dehydrogenase occurred in cell sap only. The extracellular activities probably originate to a great part from a programmed release by intact cells. Nutrient media of plant cell cultures constitute a rich source of active plant isozymes.
KW - α-galactosidase
KW - β-glucosidase
KW - acid phosphatase
KW - apple cell culture
KW - esterase
KW - intra-and extracellular enzymes
UR - http://www.scopus.com/inward/record.url?scp=34250092437&partnerID=8YFLogxK
U2 - 10.1007/BF00035755
DO - 10.1007/BF00035755
M3 - Article
AN - SCOPUS:34250092437
VL - 15
SP - 137
EP - 147
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 2
ER -