Details
| Original language | English |
|---|---|
| Pages (from-to) | 151-164 |
| Number of pages | 14 |
| Journal | Journal of Biomolecular NMR |
| Volume | 71 |
| Issue number | 3 |
| Early online date | 12 Apr 2018 |
| Publication status | Published - Jul 2018 |
Abstract
Nucleic acids play key roles in most biological processes, either in isolation or in complex with proteins. Often they are difficult targets for structural studies, due to their dynamic behavior and high molecular weight. Solid-state nuclear magnetic resonance spectroscopy (ssNMR) provides a unique opportunity to study large biomolecules in a non-crystalline state at atomic resolution. Application of ssNMR to RNA, however, is still at an early stage of development and presents considerable challenges due to broad resonances and poor dispersion. Isotope labeling, either as nucleotide-specific, atom-specific or segmental labeling, can resolve resonance overlaps and reduce the line width, thus allowing ssNMR studies of RNA domains as part of large biomolecules or complexes. In this review we discuss the methods for RNA production and purification as well as numerous approaches for isotope labeling of RNA. Furthermore, we give a few examples that emphasize the instrumental role of isotope labeling and ssNMR for studying RNA as part of large ribonucleoprotein complexes.
Keywords
- Atom selective-labelling of nucleotides, Chemical synthesis of RNA, In vitro RNA transcription, RNA structure and dynamics, Solid-state NMR
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Chemistry(all)
- Spectroscopy
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In: Journal of Biomolecular NMR, Vol. 71, No. 3, 07.2018, p. 151-164.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Isotope labeling for studying RNA by solid-state NMR spectroscopy
AU - Marchanka, Alexander
AU - Kreutz, Christoph
AU - Carlomagno, Teresa
N1 - © 2018, Springer Science Business Media B.V., part of Springer Nature
PY - 2018/7
Y1 - 2018/7
N2 - Nucleic acids play key roles in most biological processes, either in isolation or in complex with proteins. Often they are difficult targets for structural studies, due to their dynamic behavior and high molecular weight. Solid-state nuclear magnetic resonance spectroscopy (ssNMR) provides a unique opportunity to study large biomolecules in a non-crystalline state at atomic resolution. Application of ssNMR to RNA, however, is still at an early stage of development and presents considerable challenges due to broad resonances and poor dispersion. Isotope labeling, either as nucleotide-specific, atom-specific or segmental labeling, can resolve resonance overlaps and reduce the line width, thus allowing ssNMR studies of RNA domains as part of large biomolecules or complexes. In this review we discuss the methods for RNA production and purification as well as numerous approaches for isotope labeling of RNA. Furthermore, we give a few examples that emphasize the instrumental role of isotope labeling and ssNMR for studying RNA as part of large ribonucleoprotein complexes.
AB - Nucleic acids play key roles in most biological processes, either in isolation or in complex with proteins. Often they are difficult targets for structural studies, due to their dynamic behavior and high molecular weight. Solid-state nuclear magnetic resonance spectroscopy (ssNMR) provides a unique opportunity to study large biomolecules in a non-crystalline state at atomic resolution. Application of ssNMR to RNA, however, is still at an early stage of development and presents considerable challenges due to broad resonances and poor dispersion. Isotope labeling, either as nucleotide-specific, atom-specific or segmental labeling, can resolve resonance overlaps and reduce the line width, thus allowing ssNMR studies of RNA domains as part of large biomolecules or complexes. In this review we discuss the methods for RNA production and purification as well as numerous approaches for isotope labeling of RNA. Furthermore, we give a few examples that emphasize the instrumental role of isotope labeling and ssNMR for studying RNA as part of large ribonucleoprotein complexes.
KW - Atom selective-labelling of nucleotides
KW - Chemical synthesis of RNA
KW - In vitro RNA transcription
KW - RNA structure and dynamics
KW - Solid-state NMR
UR - http://www.scopus.com/inward/record.url?scp=85045289123&partnerID=8YFLogxK
U2 - 10.1007/s10858-018-0180-7
DO - 10.1007/s10858-018-0180-7
M3 - Article
C2 - 29651587
AN - SCOPUS:85045289123
VL - 71
SP - 151
EP - 164
JO - Journal of Biomolecular NMR
JF - Journal of Biomolecular NMR
SN - 0925-2738
IS - 3
ER -