Details
| Original language | English |
|---|---|
| Article number | fiw057 |
| Journal | FEMS Microbiology Ecology |
| Volume | 92 |
| Issue number | 5 |
| Publication status | Published - May 2016 |
| Externally published | Yes |
Abstract
Studies of the distribution of ammonia oxidising archaea (AOA) and bacteria (AOB) suggest distinct ecological niches characterised by ammonia concentration and pH, arising through differences in substrate affinity and ammonia tolerance. AOA form five distinct phylogenetic clades, one of which, the 'Nitrososphaera sister cluster', has no cultivated isolate. A representative of this cluster, named 'Candidatus Nitrosocosmicus franklandus', was isolated from a pH 7.5 arable soil and we propose a new cluster name: 'Nitrosocosmicus'. While phylogenetic analysis of amoA genes indicates its association with the Nitrososphaera sister cluster, analysis of 16S rRNA genes provided no support for a relative branching that is consistent with a 'sister cluster', indicating placement within a lineage of the order Nitrososphaerales. 'Ca. N. franklandus' is capable of ureolytic growth and its tolerances to nitrite and ammonia are higher than in other AOA and similar to those of typical soil AOB. Similarity of other growth characteristics of 'Ca. N. franklandus' with those of typical soil AOB isolates reduces support for niche differentiation between soil AOA and AOB and suggests that AOA have a wider physiological diversity than previously suspected. In particular, the high ammonia tolerance of 'Ca. N. franklandus' suggests potential contributions to nitrification in fertilised soils.
Keywords
- Nitrosocosmicus, Nitrososphaera sister cluster, Thaumarchaeota, ammonia inhibition, nitrification, soil
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Environmental Science(all)
- Ecology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: FEMS Microbiology Ecology, Vol. 92, No. 5, fiw057, 05.2016.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Isolation of ‘Candidatus Nitrosocosmicus franklandus’, a novel ureolytic soil archaeal ammonia oxidiser with tolerance to high ammonia concentration
AU - Lehtovirta-Morley, Laura E
AU - Ross, Jenna
AU - Hink, Linda
AU - Weber, Eva B.
AU - Gubry-Rangin, Cécile
AU - Thion, Cécile
AU - Prosser, James I.
AU - Nicol, Graeme W.
N1 - Publisher Copyright: © FEMS 2016. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016/5
Y1 - 2016/5
N2 - Studies of the distribution of ammonia oxidising archaea (AOA) and bacteria (AOB) suggest distinct ecological niches characterised by ammonia concentration and pH, arising through differences in substrate affinity and ammonia tolerance. AOA form five distinct phylogenetic clades, one of which, the 'Nitrososphaera sister cluster', has no cultivated isolate. A representative of this cluster, named 'Candidatus Nitrosocosmicus franklandus', was isolated from a pH 7.5 arable soil and we propose a new cluster name: 'Nitrosocosmicus'. While phylogenetic analysis of amoA genes indicates its association with the Nitrososphaera sister cluster, analysis of 16S rRNA genes provided no support for a relative branching that is consistent with a 'sister cluster', indicating placement within a lineage of the order Nitrososphaerales. 'Ca. N. franklandus' is capable of ureolytic growth and its tolerances to nitrite and ammonia are higher than in other AOA and similar to those of typical soil AOB. Similarity of other growth characteristics of 'Ca. N. franklandus' with those of typical soil AOB isolates reduces support for niche differentiation between soil AOA and AOB and suggests that AOA have a wider physiological diversity than previously suspected. In particular, the high ammonia tolerance of 'Ca. N. franklandus' suggests potential contributions to nitrification in fertilised soils.
AB - Studies of the distribution of ammonia oxidising archaea (AOA) and bacteria (AOB) suggest distinct ecological niches characterised by ammonia concentration and pH, arising through differences in substrate affinity and ammonia tolerance. AOA form five distinct phylogenetic clades, one of which, the 'Nitrososphaera sister cluster', has no cultivated isolate. A representative of this cluster, named 'Candidatus Nitrosocosmicus franklandus', was isolated from a pH 7.5 arable soil and we propose a new cluster name: 'Nitrosocosmicus'. While phylogenetic analysis of amoA genes indicates its association with the Nitrososphaera sister cluster, analysis of 16S rRNA genes provided no support for a relative branching that is consistent with a 'sister cluster', indicating placement within a lineage of the order Nitrososphaerales. 'Ca. N. franklandus' is capable of ureolytic growth and its tolerances to nitrite and ammonia are higher than in other AOA and similar to those of typical soil AOB. Similarity of other growth characteristics of 'Ca. N. franklandus' with those of typical soil AOB isolates reduces support for niche differentiation between soil AOA and AOB and suggests that AOA have a wider physiological diversity than previously suspected. In particular, the high ammonia tolerance of 'Ca. N. franklandus' suggests potential contributions to nitrification in fertilised soils.
KW - Nitrosocosmicus
KW - Nitrososphaera sister cluster
KW - Thaumarchaeota
KW - ammonia inhibition
KW - nitrification
KW - soil
UR - http://www.scopus.com/inward/record.url?scp=84964833242&partnerID=8YFLogxK
U2 - 10.1093/femsec/fiw057
DO - 10.1093/femsec/fiw057
M3 - Article
VL - 92
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
SN - 0168-6496
IS - 5
M1 - fiw057
ER -