Details
| Original language | English |
|---|---|
| Pages (from-to) | 278-82 |
| Number of pages | 5 |
| Journal | FEBS letters |
| Volume | 348 |
| Issue number | 3 |
| Publication status | Published - 18 Jul 1994 |
Abstract
Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.
Keywords
- Biological Transport/drug effects, Cell Membrane/chemistry, Cell Membrane Permeability, Electric Conductivity, Fabaceae/chemistry, Kinetics, Lipid Bilayers/metabolism, Membrane Potentials, Plants, Medicinal, Potassium/pharmacology, Potassium Channels/drug effects, Rubidium Radioisotopes, Tetraethylammonium, Tetraethylammonium Compounds/pharmacology
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In: FEBS letters, Vol. 348, No. 3, 18.07.1994, p. 278-82.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Isolation of a potassium-selective ion channel from the plasma membrane of the broad bean Vicia faba L
AU - Zeilinger, Carsten
PY - 1994/7/18
Y1 - 1994/7/18
N2 - Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.
AB - Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.
KW - Biological Transport/drug effects
KW - Cell Membrane/chemistry
KW - Cell Membrane Permeability
KW - Electric Conductivity
KW - Fabaceae/chemistry
KW - Kinetics
KW - Lipid Bilayers/metabolism
KW - Membrane Potentials
KW - Plants, Medicinal
KW - Potassium/pharmacology
KW - Potassium Channels/drug effects
KW - Rubidium Radioisotopes
KW - Tetraethylammonium
KW - Tetraethylammonium Compounds/pharmacology
U2 - 10.1016/0014-5793(94)00629-6
DO - 10.1016/0014-5793(94)00629-6
M3 - Article
C2 - 8034054
VL - 348
SP - 278
EP - 282
JO - FEBS letters
JF - FEBS letters
SN - 0014-5793
IS - 3
ER -