Details
| Original language | English |
|---|---|
| Article number | 244 |
| Journal | Frontiers in Plant Science |
| Volume | 3 |
| Issue number | NOV |
| Publication status | Published - 2 Nov 2012 |
Abstract
Powdery mildew is a major disease of economic importance in cut and pot roses. As an alternative to conventional resistance breeding strategies utilizing single-dominant genes or QTLs, mildew resistance locus o (MLO)-based resistance might offer some advantages. In dicots such as Arabidopsis, pea, and tomato, loss-of-function mutations in MLO genes confer high levels of broad-spectrum resistance. Here, we report the isolation and characterization of four MLO homologs from a large rose EST collection isolated from leaves. These genes are phylogenetically closely related to other dicot MLO genes that are involved in plant powdery mildew interactions. Therefore, they are candidates for MLO genes involved in rose powdery mildew interactions. Two of the four isolated genes contain all of the sequence signatures considered to be diagnostic for MLO genes. We mapped all four genes to three linkage groups and conducted the first analysis of alternative alleles. This information is discussed in regards to a reverse genetics approach aimed at the selection of rose plants that are homozygous for loss-of-function in one or more MLO genes.
Keywords
- Mildew resistance locus o, Podosphaera pannosa, Powdery mildew, Rosaceae, Tetraploid
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 3, No. NOV, 244, 02.11.2012.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Isolation, molecular characterization, and mapping of four rose MLO orthologs
AU - Kaufmann, Helgard
AU - Qiu, Xianqin
AU - Wehmeyer, Juliane
AU - Debener, Thomas
PY - 2012/11/2
Y1 - 2012/11/2
N2 - Powdery mildew is a major disease of economic importance in cut and pot roses. As an alternative to conventional resistance breeding strategies utilizing single-dominant genes or QTLs, mildew resistance locus o (MLO)-based resistance might offer some advantages. In dicots such as Arabidopsis, pea, and tomato, loss-of-function mutations in MLO genes confer high levels of broad-spectrum resistance. Here, we report the isolation and characterization of four MLO homologs from a large rose EST collection isolated from leaves. These genes are phylogenetically closely related to other dicot MLO genes that are involved in plant powdery mildew interactions. Therefore, they are candidates for MLO genes involved in rose powdery mildew interactions. Two of the four isolated genes contain all of the sequence signatures considered to be diagnostic for MLO genes. We mapped all four genes to three linkage groups and conducted the first analysis of alternative alleles. This information is discussed in regards to a reverse genetics approach aimed at the selection of rose plants that are homozygous for loss-of-function in one or more MLO genes.
AB - Powdery mildew is a major disease of economic importance in cut and pot roses. As an alternative to conventional resistance breeding strategies utilizing single-dominant genes or QTLs, mildew resistance locus o (MLO)-based resistance might offer some advantages. In dicots such as Arabidopsis, pea, and tomato, loss-of-function mutations in MLO genes confer high levels of broad-spectrum resistance. Here, we report the isolation and characterization of four MLO homologs from a large rose EST collection isolated from leaves. These genes are phylogenetically closely related to other dicot MLO genes that are involved in plant powdery mildew interactions. Therefore, they are candidates for MLO genes involved in rose powdery mildew interactions. Two of the four isolated genes contain all of the sequence signatures considered to be diagnostic for MLO genes. We mapped all four genes to three linkage groups and conducted the first analysis of alternative alleles. This information is discussed in regards to a reverse genetics approach aimed at the selection of rose plants that are homozygous for loss-of-function in one or more MLO genes.
KW - Mildew resistance locus o
KW - Podosphaera pannosa
KW - Powdery mildew
KW - Rosaceae
KW - Tetraploid
UR - http://www.scopus.com/inward/record.url?scp=84891830438&partnerID=8YFLogxK
U2 - 10.3389/fpls.2012.00244
DO - 10.3389/fpls.2012.00244
M3 - Article
AN - SCOPUS:84891830438
VL - 3
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
IS - NOV
M1 - 244
ER -