Details
| Original language | English |
|---|---|
| Pages (from-to) | 1049-1052 |
| Number of pages | 4 |
| Journal | Molecular ecology resources |
| Volume | 9 |
| Issue number | 3 |
| Publication status | Published - May 2009 |
Abstract
We provide primer sequences for 11 new polymorphic microsatellite markers developed in the tropical ant-plant genus Macaranga (Euphorbiaceae), after enrichment cloning of Macaranga tanarius and Macaranga hypoleuca. Allele numbers per locus ranged from two to 16 among 20 accessions of M. tanarius, and from three to 10 among 22 accessions of M. hypoleuca. Observed and expected heterozygosities ranged from 0.150 to 0.900 and from 0.375 to 0.894 in M. tanarius, and from 0.545 to 1.000 and from 0.434 to 0.870 in M. hypoleuca, respectively. Six of the 11 primer pairs successfully cross-amplified polymorphic polymerase chain reaction products in Macaranga winkleri.
Keywords
- Ant-plant, Cross-species amplification, Macaranga, Microsatellites, Tropical pioneer trees
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Agricultural and Biological Sciences(all)
- Ecology, Evolution, Behavior and Systematics
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
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In: Molecular ecology resources, Vol. 9, No. 3, 05.2009, p. 1049-1052.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Isolation and characterization of 11 new microsatellite markers for Macaranga (Euphorbiaceae)
AU - Baier, Christina
AU - Guicking, Daniela
AU - Prinz, Kathleen
AU - Fey-Wagner, Christina
AU - WÖhrmann, Tina
AU - Weising, Kurt
AU - Debener, Thomas
AU - Schie, Stephan
AU - Blattner, Frank R.
PY - 2009/5
Y1 - 2009/5
N2 - We provide primer sequences for 11 new polymorphic microsatellite markers developed in the tropical ant-plant genus Macaranga (Euphorbiaceae), after enrichment cloning of Macaranga tanarius and Macaranga hypoleuca. Allele numbers per locus ranged from two to 16 among 20 accessions of M. tanarius, and from three to 10 among 22 accessions of M. hypoleuca. Observed and expected heterozygosities ranged from 0.150 to 0.900 and from 0.375 to 0.894 in M. tanarius, and from 0.545 to 1.000 and from 0.434 to 0.870 in M. hypoleuca, respectively. Six of the 11 primer pairs successfully cross-amplified polymorphic polymerase chain reaction products in Macaranga winkleri.
AB - We provide primer sequences for 11 new polymorphic microsatellite markers developed in the tropical ant-plant genus Macaranga (Euphorbiaceae), after enrichment cloning of Macaranga tanarius and Macaranga hypoleuca. Allele numbers per locus ranged from two to 16 among 20 accessions of M. tanarius, and from three to 10 among 22 accessions of M. hypoleuca. Observed and expected heterozygosities ranged from 0.150 to 0.900 and from 0.375 to 0.894 in M. tanarius, and from 0.545 to 1.000 and from 0.434 to 0.870 in M. hypoleuca, respectively. Six of the 11 primer pairs successfully cross-amplified polymorphic polymerase chain reaction products in Macaranga winkleri.
KW - Ant-plant
KW - Cross-species amplification
KW - Macaranga
KW - Microsatellites
KW - Tropical pioneer trees
UR - http://www.scopus.com/inward/record.url?scp=65249101642&partnerID=8YFLogxK
U2 - 10.1111/j.1755-0998.2009.02567.x
DO - 10.1111/j.1755-0998.2009.02567.x
M3 - Article
AN - SCOPUS:65249101642
VL - 9
SP - 1049
EP - 1052
JO - Molecular ecology resources
JF - Molecular ecology resources
SN - 1755-098X
IS - 3
ER -