Details
| Original language | English |
|---|---|
| Pages (from-to) | 301-308 |
| Number of pages | 8 |
| Journal | Food chemistry |
| Volume | 216 |
| Early online date | 16 Aug 2016 |
| Publication status | Published - 1 Feb 2017 |
Abstract
Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroacetic acid 0.025% (w/v). The method demonstrated excellent linearity with a limit of detection 0.004 μg/ml. The method precision was evaluated for plant samples and it was below 0.7% (n = 6). Significant amounts of vitamin B12 in plants were detected in Hippophae rhamnoides (37 μg/100 g dry weight), in Elymus (26 μg/100 g dry weight) and in Inula helenium (11 μg/100 g dry weight).
Keywords
- Frankia alni, Hippophae rhamnoides, HPLC-MS/MS, HPLC-UV, Immunoaffinity chromatography (IAC), Plant-based foods, Vitamin B (cobalamin)
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Agricultural and Biological Sciences(all)
- Food Science
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In: Food chemistry, Vol. 216, 01.02.2017, p. 301-308.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Isolation and analysis of vitamin B12 from plant samples
AU - Nakos, M.
AU - Pepelanova, I.
AU - Beutel, S.
AU - Krings, U.
AU - Berger, R. G.
AU - Scheper, T.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroacetic acid 0.025% (w/v). The method demonstrated excellent linearity with a limit of detection 0.004 μg/ml. The method precision was evaluated for plant samples and it was below 0.7% (n = 6). Significant amounts of vitamin B12 in plants were detected in Hippophae rhamnoides (37 μg/100 g dry weight), in Elymus (26 μg/100 g dry weight) and in Inula helenium (11 μg/100 g dry weight).
AB - Based on increased demands of strict vegetarians, an investigation of vitamin B12 content in plant sources, was carried out. The vitamin B12 concentration was determined by RP-HPLC with UV detection, after prior matrix isolation by immunoaffinity chromatography (IAC). Vitamin B12 was extracted in the presence of sodium cyanide, to transform all forms of cobalamin into cyanocobalamin. Diode array detector was used to monitor vitamin B12, after its chromatographic separation under gradient elution with a mobile phase consisting of acetonitrile and trifluoroacetic acid 0.025% (w/v). The method demonstrated excellent linearity with a limit of detection 0.004 μg/ml. The method precision was evaluated for plant samples and it was below 0.7% (n = 6). Significant amounts of vitamin B12 in plants were detected in Hippophae rhamnoides (37 μg/100 g dry weight), in Elymus (26 μg/100 g dry weight) and in Inula helenium (11 μg/100 g dry weight).
KW - Frankia alni
KW - Hippophae rhamnoides
KW - HPLC-MS/MS
KW - HPLC-UV
KW - Immunoaffinity chromatography (IAC)
KW - Plant-based foods
KW - Vitamin B (cobalamin)
UR - http://www.scopus.com/inward/record.url?scp=84983372950&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2016.08.037
DO - 10.1016/j.foodchem.2016.08.037
M3 - Article
C2 - 27596424
AN - SCOPUS:84983372950
VL - 216
SP - 301
EP - 308
JO - Food chemistry
JF - Food chemistry
SN - 0308-8146
ER -