Details
| Original language | English |
|---|---|
| Article number | 178019 |
| Journal | Science of the Total Environment |
| Volume | 958 |
| Early online date | 13 Dec 2024 |
| Publication status | E-pub ahead of print - 13 Dec 2024 |
Abstract
Rice rhizosphere soil is a hotspot of microbial activity and a complex interplay between soil abiotic properties, microbial community and organic carbon (C). The iron (Fe) plaque formation in the rice rhizosphere promotes Fe-bound organic C formation and increases microbial activity. Yet, the overall impact of Fe on C storage via physicochemical stabilization and microbial mineralization of rhizodeposits (rhizo-C) and soil organic C (SOC) in the rice rhizosphere remain unclear. We conducted a microcosm experiment using 13C-CO2 pulse labeling to grow rice (Oryza sativa L.) with four levels of α-FeOOH addition (Control, Fe-10 %, Fe-20 %, Fe-40 % w/w of α-FeOOH per total Fe in soil). This study aimed to evaluate the impact of Fe oxides on rhizo-C mineralization, the rhizosphere priming effect, and Fe-OM formation. Microbial community composition and localization of enzyme activities were also quantified through 16S rRNA sequencing and zymography. The hotspot area, as being indicated by zymography, increased by 20-50% in the presence of Fe compared to the soil without Fe addition. Despite being a hotspot, strong coprecipitation of Fe-OM in the rhizosphere promoted C immobilisation. Fe-20 % and Fe-40 % resulted in a 41 % and 33 % decrease of rhizodeposits derived 13C-CO2 emission and increased 13C stabilization mainly in 0.25–2 mm soil aggregates due to coprecipitation and aggregate formation with α-FeOOH. Moreover, Fe addition led to a dominance of Fe-oxidizing bacteria genera such as Pseudomonas, which fostered coprecipitation of Fe-OM formation. We highlight larger physicochemical stabilization of organic C by α-FeOOH addition despite raised hotspot area of microbial activity in the rice rhizosphere.
Keywords
- Carbon accrual, Fe oxidizing bacteria, Hotspot, Iron plaque, Shannon index, Zymography
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Engineering
- Environmental Science(all)
- Environmental Chemistry
- Environmental Science(all)
- Waste Management and Disposal
- Environmental Science(all)
- Pollution
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In: Science of the Total Environment, Vol. 958, 178019, 01.01.2025.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Iron oxides promote physicochemical stabilization of carbon despite enhancing microbial activity in the rice rhizosphere
AU - Sun, Han
AU - Ma, Xiaomin
AU - Van Zwieten, Lukas
AU - Luo, Yu
AU - Brown, Robert W.
AU - Guggenberger, Georg
AU - Tang, Sheng
AU - Kuzyakov, Yakov
AU - Jeewani, Peduruhewa H.
N1 - Publisher Copyright: © 2024 Elsevier B.V.
PY - 2024/12/13
Y1 - 2024/12/13
N2 - Rice rhizosphere soil is a hotspot of microbial activity and a complex interplay between soil abiotic properties, microbial community and organic carbon (C). The iron (Fe) plaque formation in the rice rhizosphere promotes Fe-bound organic C formation and increases microbial activity. Yet, the overall impact of Fe on C storage via physicochemical stabilization and microbial mineralization of rhizodeposits (rhizo-C) and soil organic C (SOC) in the rice rhizosphere remain unclear. We conducted a microcosm experiment using 13C-CO2 pulse labeling to grow rice (Oryza sativa L.) with four levels of α-FeOOH addition (Control, Fe-10 %, Fe-20 %, Fe-40 % w/w of α-FeOOH per total Fe in soil). This study aimed to evaluate the impact of Fe oxides on rhizo-C mineralization, the rhizosphere priming effect, and Fe-OM formation. Microbial community composition and localization of enzyme activities were also quantified through 16S rRNA sequencing and zymography. The hotspot area, as being indicated by zymography, increased by 20-50% in the presence of Fe compared to the soil without Fe addition. Despite being a hotspot, strong coprecipitation of Fe-OM in the rhizosphere promoted C immobilisation. Fe-20 % and Fe-40 % resulted in a 41 % and 33 % decrease of rhizodeposits derived 13C-CO2 emission and increased 13C stabilization mainly in 0.25–2 mm soil aggregates due to coprecipitation and aggregate formation with α-FeOOH. Moreover, Fe addition led to a dominance of Fe-oxidizing bacteria genera such as Pseudomonas, which fostered coprecipitation of Fe-OM formation. We highlight larger physicochemical stabilization of organic C by α-FeOOH addition despite raised hotspot area of microbial activity in the rice rhizosphere.
AB - Rice rhizosphere soil is a hotspot of microbial activity and a complex interplay between soil abiotic properties, microbial community and organic carbon (C). The iron (Fe) plaque formation in the rice rhizosphere promotes Fe-bound organic C formation and increases microbial activity. Yet, the overall impact of Fe on C storage via physicochemical stabilization and microbial mineralization of rhizodeposits (rhizo-C) and soil organic C (SOC) in the rice rhizosphere remain unclear. We conducted a microcosm experiment using 13C-CO2 pulse labeling to grow rice (Oryza sativa L.) with four levels of α-FeOOH addition (Control, Fe-10 %, Fe-20 %, Fe-40 % w/w of α-FeOOH per total Fe in soil). This study aimed to evaluate the impact of Fe oxides on rhizo-C mineralization, the rhizosphere priming effect, and Fe-OM formation. Microbial community composition and localization of enzyme activities were also quantified through 16S rRNA sequencing and zymography. The hotspot area, as being indicated by zymography, increased by 20-50% in the presence of Fe compared to the soil without Fe addition. Despite being a hotspot, strong coprecipitation of Fe-OM in the rhizosphere promoted C immobilisation. Fe-20 % and Fe-40 % resulted in a 41 % and 33 % decrease of rhizodeposits derived 13C-CO2 emission and increased 13C stabilization mainly in 0.25–2 mm soil aggregates due to coprecipitation and aggregate formation with α-FeOOH. Moreover, Fe addition led to a dominance of Fe-oxidizing bacteria genera such as Pseudomonas, which fostered coprecipitation of Fe-OM formation. We highlight larger physicochemical stabilization of organic C by α-FeOOH addition despite raised hotspot area of microbial activity in the rice rhizosphere.
KW - Carbon accrual
KW - Fe oxidizing bacteria
KW - Hotspot
KW - Iron plaque
KW - Shannon index
KW - Zymography
UR - http://www.scopus.com/inward/record.url?scp=85211593618&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2024.178019
DO - 10.1016/j.scitotenv.2024.178019
M3 - Article
AN - SCOPUS:85211593618
VL - 958
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 178019
ER -